The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Salviae miltiorrhizae on Inflammatory Cells Associated with Asthma via Splenocyte, BMMC, Eosinophil

Splenocyte, BMMC, eosinophil을 통해 본 단삼(丹蔘)이 천식 관련 염증세포에 미치는 영향

  • Jeong, Seung-Yeon (Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung-hee University) ;
  • Kim, Jin-Ju (Dept. of Oriental Physiology, College of Pharmacy, Kyung-hee University) ;
  • Jung, Hee-Jae (Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung-hee University) ;
  • Jung, Sung-Ki (Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung-hee University)
  • 정승연 (경희대학교 한의과대학 폐계내과학 교실) ;
  • 김진주 (경희대학교 약학대학 한방생리학교실) ;
  • 정희재 (경희대학교 한의과대학 폐계내과학 교실) ;
  • 정승기 (경희대학교 한의과대학 폐계내과학 교실)
  • Published : 2009.03.31
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Abstract

Objectives : Asthma is a chronic inflammatory disorder of the airways by many cells such as mast cells, Th2 lymphocytes and eosinophile. The present study was aimed to evaluate the effects of Salviae miltiorrhizae (SM) on T cell cytokine production, mast cells. and eosinophils, Methods : We screened 13 herbs to find compounds with potential to control Th cytokine production. using concanavalin A (con A)-activated splenocyte cultures. Con A-activated $IFN-\gamma$ and IL-4 levels in supernatants of splenocyte cultures. Bone marrow derived mast cells (BMMC) were incubated with SM and then the expressions of membrane proteins of BMMC were analyzed by fluorescence activated cell sorter (FACS). BALB/c mice sensitized to ovalbumin (OVA) were challenged with aerosolized OVA for 6 weeks. During the last weeks some mice were treated with SM. Then eosinophils in bronchoalveolar lavage fluid (BALf) were counted and pathologic changes of lung tissue were observed with hematoxylin-eosin stain. Results : SM increased $IFN-\gamma$ level on splenocyte culture significantly. but had no significant effects on expressions of ICAM-1, CD62L, integrin $a_4$. c-kit, IL-3 receptors. CD11a, or IgE receptors of BMMC. SM treatment significantly inhibited eosinophil infiltrates in BALf and peribronchial lung inflammation. Conculusions : The present data suggested that SM may have an effect on Th cytokine secretion and eosinophils associated with asthma responses. Therefore SM might be of therapeutic value in treating asthma.

Keywords

References

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