Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic Diversity of Xanthomonas arboricola pv. pruni Isolated in Korea

우리나라에서 분리한 Xanthomonas arboricola pv. pruni의 유전적 다양성

  • Published : 2009.05.30
Download PDF
⟨ Previous Next ⟩

Abstract

Xanthomonas arboricola pv. pruni, the causal agent of bacterial shot holes in stone fruits, was known to have a low population diversity. To investigate the genetic characteristics of X. arboricola pv. pruni isolated in Korea, three strains which have identical 16S rDNA sequences - including type strain (LMG852), Japanese isolate (MAFF301420) and Korean isolate (XWD1) - were analysed based on the nucleotide sequences of three DNA regions and RAPD pattern. No sequence diversity among the three strains was found within the ITS, glnA and atpD gene sequences. However, five of 756 nucleotides of the atpD gene determined (accession number FJ429319) were different from those of the French strain available from the Genbank database. RAPD analyses performed with 40 different arbitrary primers revealed that two strains isolated from Korea and Japan showed similarity in their band patterns distinguished by type strain. These results suggest that Korean and Japanese strains are very close and belong to a population with a low genetic diversity, and might have a different origin from strains found in West Europe.

핵과류 과수에 세균성구멍병을 일으키는 Xanthomonas arboricola pv. pruni는 집단의 다양성이 적은 것으로 알려지고 있다. 우리나라에서 분리된 X. arboricola pv. pruni의 유전적 특성을 조사하기 위하여 동일한 16S rDNA 염기서열을 갖는 X. arboricola pv. pruni의 type strain인 LMG852, 일본 균주 MAFF301420, 우리나라 균주 XWD1의 세 균주를 대상으로 세 개 유전자 부위의 DNA 염기서열과 RAPD 분석을 실시하였다. 그 결과 ITS와 glnA, atpD의 염기서열은 세 균주가 동일하였다. 그러나 756 염기로 구성된 atpD의 염기서열은 GenBank에 등록된 프랑스균주와 5곳에서 차이가 있었다. 40개의 random primer를 사용한 RAPD 결과는 우리나라와 일본균주는 동일한 밴드 패턴을 보이나 대표균주와는 다른 양상을 보였다. 이러한 사실은 우리나라와 일본의 X. arboricola pv. pruni의 개체군은 매우 가까워 유전적 다양성이 낮은 것으로 보이며 유럽균주와는 다른 기원과 전파 경로를 갖는 것으로 생각된다.

Keywords

References

  1. Battilani, P., V. Rossi, and A. Saccardi. 1999. Development of Xanthomonas arboricola pv. pruni epidemics on peaches. J. Plant Pathol. 81, 161-171
  2. Boudon, S., C. Manceau, and J. -L. Notteghem. 2005. Structure and origin of Xanthomonas arboricola pv. pruni populations causing bacterial spot of stone fruit trees in Western Europe. Phytopathology 95, 1081-1088 https://doi.org/10.1094/PHYTO-95-1081
  3. Choi, J. E., E. J. Lee, and Y. S. Park. 2000. Shot hole of peach and Japanese plum caused by Xanthomonas campestris pv. pruni and Erwinia nigrifluens in Korea. Plant Dis. Res. 6, 10-14
  4. Christensen, H. and J. E. Olsen. 1998. Phylogenetic relationships of Salmonella based on DNA sequence comparison of atpD encoding $\beta$ subunit of ATP synthase. FEMS Microbiol. Lett. 161, 89-96
  5. Du Plessis, H. J. 1988. Differential virulence of Xanthomonas arboricola pv. pruni to peach, plum, and apricot cultivars. Phytopathology 78, 1312-1315 https://doi.org/10.1094/Phyto-78-1312
  6. EPPO/CABI. 1997. Xanthomonas arboricola pv. pruni. pp. 1096-1100. 2nd eds. In Quarantine pests for Europe, CAB International, Wallingford, GB
  7. Forsman, P., A. Tilsala-Timisjarvi, and T. Atatossava. 1997. Identification of staphylococcal and streptococcal causes of bovine mastitis using 16S-23S rRNA spacer regions. Microbiol. 143, 3491-3500 https://doi.org/10.1099/00221287-143-11-3491
  8. Goncalves, E. R. and Y. B. Rosato. 2002. Phylogenetic analysis of Xanthomonas species based upon 16S-23S rDNA intergenic spacer sequences. Int. J. Sys. Evol. Microbiol. 52, 355-361
  9. Jami, F., M. N. Kazempour, S. A. Elahinia, and G. Khodakaramian. 2005. First report of Xanthomonas arboricola pv. pruni on stone fruit trees from Iran. J. Phytopathol. 153, 371-372 https://doi.org/10.1111/j.1439-0434.2005.00975.x
  10. Pezole, G., C. Gissi, C. Lanave, and C. Saccone. 1995. Glutamine synthetase gene evolution in bacteria. Mol. Biol. Evol. 12, 189-197
  11. Ritchie, D. F. 1995. Bacterial spot. pp. 50-52, In Ogawa, J. M., E. I. Zehr, G. W. Bird, D. F. Ritchie, K. Uriu, and J. K. Uyemoto (eds.), Compendium of stone fruit diseases. The American Pathological Society, St. Paul, Mn.
  12. Smith, E. F. 1903. Observations on a hither to unreported bacterial disease, the cause of which enters the plant through ordinary stomata. Science 17, 456-457
  13. Solca, N. M., M. V. Bernasconi, C. Valsangiacomo, L. -J. V. Van Doorn, and J. C. Piffaretti. 2001. Population genetics of Helicobacter pylori in the southern part of Switzerland analyzed by sequencing of four housekeeping genes (atpD, glnA, scoB and recA), and by vacA, cagA, iceA and IS605 genotyping. Microbiology 147, 1693-1707
  14. Stackebrandt, E. and W. Liesack. 1993. Nucleic acids and classification. pp. 152-189. In Goodfellow, M. and A. G. O'Donnell (eds.), Handbook of New Bacterial Systematics, Academic Press, London
  15. Zaccardelli, M., P. Ceroni, and U. Mazzucchi. 1999. Amplified fragment length polymorphism fingerprinting of Xanthomonas arboricola pv. pruni. J. Plant Pathol. 81, 173-179 https://doi.org/10.1590/S1517-83822010000300026