생명과학회지 (Journal of Life Science)

  • 제19권5호
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  • Pages.680-683
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  • 2009
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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대나무로부터 분리한 linoleic acid의 α-glucosidase 저해활성 연구

Linoleic Acid from Bamboo (Phyllostachys Bambusoides) Displaying Potent α- Glucosidase Inhibition

  • 정선인 (경상대학교 응용생명과학부) ;
  • 강수태 (주식회사 청학동 삼선당) ;
  • 최철용 (주식회사 청학동 삼선당) ;
  • 오경열 (경상대학교 응용생명과학부) ;
  • 조정근 (경상대학교 응용생명과학부) ;
  • ;
  • 박기훈 (경상대학교 응용생명과학부)
  • Jung, Sun-In (Division of Applied Life Science (BK21 Program), EB-NCRC, Institute of Agriculture & Life Science, Graduate School of Gyeongsang National University) ;
  • Kang, Su-Tae (Cheonghak-Dong Samsundang Food Co.) ;
  • Choi, Cheol-Yong (Cheonghak-Dong Samsundang Food Co.) ;
  • Oh, Kyeong-Yeol (Division of Applied Life Science (BK21 Program), EB-NCRC, Institute of Agriculture & Life Science, Graduate School of Gyeongsang National University) ;
  • Cho, Jung-Keun (Division of Applied Life Science (BK21 Program), EB-NCRC, Institute of Agriculture & Life Science, Graduate School of Gyeongsang National University) ;
  • Rengasamy, Rajesh (Division of Applied Life Science (BK21 Program), EB-NCRC, Institute of Agriculture & Life Science, Graduate School of Gyeongsang) ;
  • Park, Ki-Hun (Division of Applied Life Science (BK21 Program), EB-NCRC, Institute of Agriculture & Life Science, Graduate School of Gyeongsang National University)
  • 발행 : 2009.05.30
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초록

당가수분해효소 저해제는 2형 당뇨병, 암, 바이러스 감염제 개발의 주요 타켓화합물이 되고 있다. 본 연구에서는 기능성식품 소재로 이용될 수 있는 대나무 잎에서 ${\alpha}$-glucosidase 저해제를 탐색하였다. 대나무 잎을 메탄올 용매로 추출하고, 이들을 핵산, 클로로포름, 부탄올로 용매분획 하였다. 용매 분획된 각 추출물의 ${\alpha}$-glucosidase 저해활성을 검정시험에서 핵산 분획층에서 강한 ${\alpha}$-glucosidase 저해활성(IC50 33.5 ${\mu}$g/ml)을 관찰하였다. 높은 저해활성을 보여준 핵산 분획층의 활성물질은 linoleic acid로 구조동정 되었다. 분리된 linoleic acid는 IC50이 12.4 ${\mu}$M로 높은 ${\alpha}$- glucosidase 저해활성을 나타내었다. 저해활성 메커니즘 연구에서 linoleic acid는 비경쟁적 저해 양상을 나타내었다. 본 연구는 대나무가 ${\alpha}$-glucosidase 저해활성을 나타내는 첫 번째 연구 결과이다.

Glycosidase inhibitors are major targets in the treatment of type II diabetes, cancer and viral infections. This study was carried out to investigate the glycosidase inhibitory substances from bamboo (Phyllostachys bambusoides). Bamboo was extracted with methanol and then further fractionated with n-hexane, chloroform, n-BuOH and aqueous to get an active fraction. All extracts were evaluated for ${\alpha}$-glucosidase inhibitory activities to identify the n-hexane fraction with 33.5 ${\mu}$g/ml of IC50 value. Active compound 1 in the n-hexane fraction was identified as linoleic acid, which exhibited inhibitory activity with 12.4 ${\mu}$M of IC50 value. Mechanistic analysis showed that linoleic acid exhibited noncompective inhibition. This is the first study in which bamboo is reported to show ${\alpha}$-glucosidase inhibitory activity.

키워드

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