생명과학회지 (Journal of Life Science)

  • 제19권5호
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  • Pages.671-675
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  • 2009
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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필발(Piper longum Linn.)로부터 전립선암 세포사멸물질 pipernonaline의 분리 및 동정

Purification and Identification of Apoptosis Modulator Pipernonaline from Piper longum Linn. against Prostate Cancer Cells

  • 김광연 (부산대학교 의학전문대학원) ;
  • 김윤진 (부산대학교 의학전문대학원) ;
  • 이완 (부산대학교병원 비뇨기과) ;
  • 유선녕 (부산대학교 의학전문대학원) ;
  • 조효진 (국립암센타) ;
  • 이선이 (농촌진흥청 국립원예특작과학원) ;
  • 이한승 (신라대학교 의생명과학대학 바이오식품소재학과) ;
  • 손재학 (신라대학교 의생명과학대학 바이오식품소재학과) ;
  • 오현철 (신라대학교 의생명과학대학 바이오식품소재학과) ;
  • 안순철 (신라대학교 의생명과학대학 바이오식품소재학과)
  • Kim, Kwang-Youn (Pusan National University School of Medicine) ;
  • Kim, Yun-Jin (Pusan National University School of Medicine) ;
  • Lee, Wan (Department of Urology, Pusan National University Hospital) ;
  • Yu, Sun-Nyoung (Pusan National University School of Medicine) ;
  • Cho, Hyo-Jin (Division of Translational Clinical Research I, National Cancer Center) ;
  • Lee, Sun-Yi (Rural Development Administration, National Institute Horticultural & Herbal Science) ;
  • Lee, Han-Seung (Department of Bio-Food Materials, College of Medical and Life Sciences, Silla University) ;
  • Sohn, Jae-Hak (Department of Bio-Food Materials, College of Medical and Life Sciences, Silla University) ;
  • Oh, Hyuncheol (Department of Bio-Food Materials, College of Medical and Life Sciences, Silla University) ;
  • Ahn, Soon-Cheol (Pusan National University School of Medicine)
  • 발행 : 2009.05.30
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초록

Prostate cancer has been a critical health problem due to an increase of prostate cancer-related deaths worldwide. Also, a frequent treatment option for prostate cancer is androgen ablation, but this treatment has a limited scope, especially for hormone-refractory cancer. There is an urgent need for the identification of alternative therapeutic strategies for prostate cancer. Previously, over one hundred species of dried-plant methanol extracts were tested for inhibitory effects on proliferation. One of them, Piper longum Linn. was selected based on its potent anti-proliferation effect. The dried root of P. longum Linn. was extracted with 100% methanol for 2-3 days and its extract was fractionated using chloroform. The chloroform layer was then subjected to column chromatography on silica gel, reverse phase-18 (RP-18) and Sephadex LH-20, in turn. Finally, the pure compound was obtained and identified as pipernonaline by NMR spectroscopic and physico-chemical analysis. In this study, anti-proliferation and cell cycle arrest effects of pipernonaline on human prostate cancer PC-3 cells were investigated using the MTT and PI staining, respectively. Our findings suggest that pipernonaline represents a dose-dependent growth inhibition pattern on PC-3 cells and, moreover, its growth inhibition is associated with sub-G1 and G0/G1 cell cycle accumulation in PC-3 cells. Also, these results provide an anticancer candidate for human prostate cancer.

키워드

참고문헌

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피인용 문헌

  1. Regulation of Pipernonaline on Biological Functions of Human Prostate Cancer Cells Based on Microarray Analysis vol.22, pp.11, 2012, https://doi.org/10.5352/JLS.2012.22.11.1552