생명과학회지 (Journal of Life Science)

  • 제19권5호
  • /
  • Pages.652-658
  • /
  • 2009
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

두릅 순 및 잎 추출물의 항산화 효과

Antioxidative Activity of Aralia elata Shoot and Leaf Extracts

  • Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Ahn, Hee-Young (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Eom, Kyung-Eun (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Park, Bo-Kyung (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Jun, Bang-Sil (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Cho, Young-Su (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
  • 발행 : 2009.05.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

두릅 순 및 두릅 잎의 천연 항산화제 소재 개발을 목적으로 생리활성물질 분석과 항산화 활성을 DPPH free radical 소거 활성능, 불포화 지방산인 linoleic acid를 이용한 thiocyanate법 및 TBA법으로 지질과산화 정도를 측정 하였다. 두릅 순 및 두릅 잎으로 부터 추출된 수율은 수용성 3.08% 및 3.13%, 에탄올 추출물 0.58% 및 0.66%, 메탄올 추출물 0.81% 및 1.73%로 용매 중에서는 메탄올 추출에서 수율이 높았으며, 두릅 순 보다는 두릅 잎에서 추출 수율이 높았다. 두릅순의 총 폴리페놀 화합물 함량은 수용성 4.68%, 에탄올 6.60% 및 메탄올 5.00%로 에탄올 추출물에서 가장 높았으며, 두릅 잎에서는 수용성 10.74%, 에탄올 16.20% 및 메탄올 16.68%로 메탄올 추출물에서 가장 높은 함량을 보였고, 두릅 순 보다는 두릅 잎에서 총 폴리페놀 화합물 함량이 높았다. 미네랄 함량은 두릅 순 및 두릅 잎에서 Ca이 각각 575.7 및 759.3 mg%로 가장 많이 함유되었고, 다음으로 K가 353.5 및 330.0 mg%, Mg가 31.3 및 31.0 mg%였으며, Zn, Mn, Na, Fe 성분은 소량씩 함유되어 있었다. DPPH free radical 소거 활성능은 두릅 순 수용성 추출물 0.1% 농도에서 28.69%로 에탄올과 메탄올 추출물보다 높았으며, 두릅 잎 추출물 0.1% 첨가구 에서 수용성 추출물 54.86%, 에탄올 추출물 89.77%, 메탄올 추출물 92.36%로 메탄올 추출물에서 가장 높게 나타났다. DPPH free radical 소거 활성능 측정에서는 두릅 잎 메탄올 추출물 0.1% 첨가가 시판 합성 항산화제인 BHT 0.05% 처리의 92.29%와 비슷한 수준으로 가장 높은 전자공여능을 나타내었다. 불포화 지방산인 linoleic acid를 이용한 thiocyanate법으로 항산화 활성을 측정한 결과에서는 두릅잎 에탄올 추출물에서 높았으며, TBA법 항산화 활성 측정에서도 두릅순과 두릅 잎 에탄올 추출물에서 각각 우수한 항산화 활성을 보였다. 이상의 실험결과에서 항산화 활성과 밀접한 관련성을 가지고 있는 총 폴리페놀 화합물 함량, DPPH free radical 소거 활성능, 불포화 지방산인 linoleic acid를 이용한 thiocyanate법 및 TBA법으로 지질과산화 정도는 두릅 순 추출물보다는 두릅 잎 추출물에서 더 우수한 항산화 활성을 가진 것으로 나타나 향후 천연 항산화제 소재 개발에 유용하게 사용될 것으로 사료되어 진다.

The comparative activities of aqueous, ethanol, and methanol extracts from Aralia elata shoot (AES) and leaf (AEL) were tested by in vitro experimental models of linoleic acid peroxidation by thiocyanate and thiobarbituric acid (TBA) methods and scavenging activities of free radicals by DPPH (${\alpha}$,${\alpha}$'-diphenyl-${\beta}$-picrylhydrazyl). In addition, bio-active materials (phenolic compounds and minerals) were also measured. The extract yield of each solvent extracted from AES and AEL was 3.08% and 3.13% in aqueous, 0.58% and 0.66% in ethanol, and 0.81% and 1.73% in methanol, respectively. The highest extract yield was found in the aqueous extract from AEL. Major mineral contents (mg%) of AES and AEL were 575.7 and 759.3 in Ca, 353.5 and 330.0 in K, and 31.3 and 31.0 in Mg, respectively. The highest free radical scavenging activity was found in the aqueous extract by 28.69% at 0.1% additional level from AES and in the methanol extract by 92.36% at 0.1% additional level from AEL. Free radical scavenging activity was stronger in AEL than in AES. In antioxidative activities determined by thiocyanate and TBA methods against lipid peroxidation using linoleic acid, ethanol extracts from AEL showed the highest antioxidative activity at all treatment concentrations. These results may provide the basic data to understand the biological activities of bio-active materials derived from AES and AEL.

키워드

참고문헌

  1. Ahmed, K. A., M. Sekaran Muniandy, and I. S. Ismail. 2009. $N^\varepsilon$-(Carboxymethyl)lysine and Coronary Atherosclerosis-Associated Low Density Lipoprotein Abnormalities in Type 2 Diabetes: Current Status. J. Clin. Biochem. Nutr. 44, 14-27 https://doi.org/10.3164/jcbn.08-190
  2. AOAC. 1975. official methods of analysis. 12th ed., Association of official analytical chemists. Washington, D.C., U.S.A
  3. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1204 https://doi.org/10.1038/1811199a0
  4. Cha, J. Y., H. J. Kim, C. H. Chung, and Y. S. Cho. 1999. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J. Korean Soc. Food Sci. Nutr. 28, 1310-1315
  5. Cha, J. Y., J. J. Jeong, Y. T. Kim, W. S. Seo, H. J. Yang, J. S. Kim, and Y. S. Lee. 2006. Detection of chemical characteristics in Hamcho (Salicornia herbacea) according to harvest periods. J. Life Sci. 16, 683-690 https://doi.org/10.5352/JLS.2006.16.4.683
  6. Cha, J. Y. and Y. S. Cho. 1999. Effect of potato polyphenolics on lipid peroxidation in rats. J. Korean Soc. Food Sci. Nutr. 28, 1131-1136
  7. Cha, J. Y., J. S. Heo, B. K. Park, H. Y. Ahn, B. S. Jun, and Y. S. Cho. 2008. Antioxidative activity of zinc-enriched Saccharomyces cerevisiae FF-10 in in vitro model system. J. Life Sci. 19, 179-184
  8. Cha, J. Y., S. H. Park, J. S. Heo, and Y. S. Cho. 2008. Suppressive effect of administrated glutathione-enriched Saccharomyces cerevisiae FF-8 on the oxidative stress in alcoholic fatty liver. J. Life Sci. 18, 1053-1058
  9. Cha, J. Y. and Y. S. Cho. 1999. Effect of potato polyphenolics on lipid peroxidation in rats. J. Korean Soc. Food Sci. Nutr. 28, 1131-1136
  10. Chang, Y. S., U. Choi, D. H. Shin, and J. I. Shin. 1992. Synergistic of Rhus javanica L. ethanol extract containing several synergist. Korean J. Food Sci. Technol. 24, 149-153
  11. Chen, Y. T., R. L. Zheng, Z. J. Jia, and J. Ju. 1990. Flavonoids as superoxide scavengers and antioxidants. Free Radical Biol. Med. 9, 19-21
  12. Choi, U. D., H. Shin, Y. S. Chang, and J. I. Shin. 1992. Screening of natural antioxidant from plant and antioxidant effect. Korean J. Food Sci. Technol. 24, 142-148
  13. Choi, H. J., W. S. Lee, S. J. Hwang, I. J. Lee, D. H. Shin, H. Y. Kim, and K. U. Kim. 2000. Changes in chemical compositions of green tea under the different extraction conditions. J. Life Sci. 10, 202-209
  14. Choi, M. S., D. H. Do, and D. J. Choi. 2002. The effect of mixing beverage with Aralica continentalis Kitagawa root on blood pressure and blood constituents of the diabetic and hypertensive elderly. Korean J. Food Nutr. 15, 165-172
  15. Chung, C. K. and M. E. Jung. 2003. Ethanol fraction of Aralia elata Seemann enhances antioxidant activity and lowers serum lipids in rats when administered with benzo(a)pyrene. Biol. Pharm. Bull 26, 1502-1504 https://doi.org/10.1248/bpb.26.1502
  16. Chung, S. H., K. D. Moon, J. K. Kim, J. H. Seong, and T. H. Sohon. 1994. Changes of chemical components in persimmon leaves during growth for processing persimmon leaves tea. Korean J. Food Sci. Technol. 26, 141-146
  17. Creager, M. A., T. F. Luscher, F. Cosentino, and J. A. Beckman. 2003. Diabetes and vascular disease: pathophysiology, clinical consequences, and medical therapy: Part I. Circulation 108, 1527-1532 https://doi.org/10.1161/01.CIR.0000091257.27563.32
  18. Han, G. J., D. S. Shin, and M. S. Jang. 2008. A study of the nutritional composition of Aralica continentalis Kitagawa and Aralica continentalis Kitagawa leaf. Korean J. Food Sci. Technol. 40, 680-685
  19. Han, G. J., M. S. Jang, and D. S. Shin. 2007. Changes in the quality characteristics of Aralica continentalis Kitagawa pickle during storage. Korean J. Food Cookery Sci. 23, 294-301
  20. Han, G. J. and M. S. Jang. 2008. Quality characteristics of Aralica continentalis Kitagawa leaf-kimchi as affected by storage time. J. Korean Soc. Food Sci. Nutr. 37, 1202-1207 https://doi.org/10.3746/jkfn.2008.37.9.1202
  21. Helmersson, J., J. Arnlov, A. Larsson, and S. Basu. 2008. Low dietary intake of beta-carotene, alpha-tocopherol and ascorbic acid is associated with increased inflammatory and oxidative stress status in a Swedish cohort. Br. J. Nutr. 15, 1-8 https://doi.org/10.1079/BJN19610003
  22. Hwang, Y. P., J. H. Choi, and H. G. Jeong. 2009. Protective effect of the Aralia continentalis root extract against carbon tetrachloride-induced hepatotoxicity in mice. Food Chem. Toxicol. 47, 75-81 https://doi.org/10.1016/j.fct.2008.10.011
  23. Jialal, I. and S. Grundyl. 1992. Effects of dietary supplementation with alpha-tocopherol on the oxidative modification of low density lipoprotein. J. Lipid Res. 33, 899-906 https://doi.org/10.1172/JCI118848
  24. Johnson, J. E., R. Walford, D. Harma, and J. Miquel. 1986. In `Free radicals, aging and degenerative disease', Alen R. Liss, N.Y.
  25. Jung, W. S., C. Y. Yu, J. G. Park, M. J. Kim, J. H. Kim, and J. K. Kim. 2006. Comparison of biological activities in extracts from Oplopanax elatus. Korean J. Medicinal Crop Sci. 14, 630-631
  26. Kim, E. Y., I. H. Baik, J. H. Kim, S. R. Kim, and M. R. Rhyu. 2004. Screening of the antioxidant activity od some medicinal plants. Korean J. Food Sci. Technol. 36, 333-338
  27. Kim, H. J., B. S. Jun, S. K. Kim, J. Y. Cha, and Y. S. Cho. 1998. Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctorius L.). J. Korean Soc. Food Sci. Nutr. 27, 1217-1222
  28. Kim, J. Y., Y. S. Maeng, and K. Y. Lee. 1995. Antioxidative effects of soybean extracts by using various solvents. Korean J. Food Sci. Technol. 27, 635-637
  29. Kim, N. H., D. C. Yang, and A. H. Eom. 2004. A phylogenetic relationships of Araliaceae based on PCR-RAPD and ITS sequences. Korean J. Plant Res. 17, 82-93
  30. Kim, Y. H. and J. G. Im. 1999. Effect of sapanin from the shoot of Aralia elata in normal rats and streptozotocin induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 28, 912-916
  31. Kim, Y. S., M. R. Huh, and J. C. Park. 2001. Effects of culture media and seawater on growth and mineral concentrations in glasswort (Salicornia herbacea). Korean J. Hort. Sci. Technol. 19, 342-347
  32. Koskas, J. P., J. Cillard, and P. Cillard. 1984. Autoxidation of linoleic acid and behavior of its hydroperoxides with and without tocopherols. JAOCS 61, 1467-1472
  33. Kuhnau, J. 1976. The flavonoids; a class of semiessential food components; their role in human nutrition. World Rev. Nutr. Diet 24, 117-120
  34. Lee, J. H. and S. R. Lee. 1994. Analysis of phenolic substances content on Korea plant foods. Korean J. Food Sci. Technol. 26, 310-316
  35. Lee, S. O., H. J. Lee, M. H. Yu, H. G. Im, and I. S. Lee. 2005. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung Island. Korean J. Food Sci. Technol. 37, 233-243
  36. Ma, S. J., B. S. Ko, and K. H. Park. 1995. Isolation of 3,4-dihydroxybenzoic acid with antimicrobial activity from bark of Aralia elata. Korean J. Food Sci. Thechnol. 27, 807-812
  37. Ohkawa, H., N. Ohishi, and K. Yagi. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  38. Shin, K. H. 2006. Effects of Araliaceae on lipid levels of plasma and liver in streptozotocin-induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 35, 1172-1177 https://doi.org/10.3746/jkfn.2006.35.9.1172
  39. Song, S. J., N. Nakamura, C. M. Ma, M. Hattori, and S. X. Xu. 2001. Five saponins from the root bark of Aralica elata. Phytochemistry 56, 491-497 https://doi.org/10.1016/S0031-9422(00)00379-4
  40. Suh, S. J., U. H. Jin, K. W. Kim, J. K. Son, S. H. Lee, K. H. Son, H. W. Chang, Y. C. 00. Lee, and C. H. Kim. 2007. Triterpenoid saponin, oleanolic acid 3-O-$\beta$-d-glucopyranosyl(1→3)-$\alpha$-l-rhamnopyranosyl(1→2)-$\alpha$-l-arabinopyranoside (OA) from Aralia elata inhibits LPS-induced nitric oxide production by down-regulated NF-κB in raw 264.7 cells. Arch. Biochem. Biophys. 497, 227-233
  41. Swain, T., W. E. Hillis, and M. Oritega. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10, 83-88
  42. Terao, J. 1989. Autoxidation activity of $\beta$-carotene-related carotenoids in solution. Lipids 24, 657-661
  43. Vinson, J. A. and B. A. Hontz. 1984. Phenol antioxidative index: Comparative antioxidant effectiveness of red and white wines. J. Agric. Food Chem. 43, 401-403
  44. Wong, S. F., B. Holliwell, R. Richimond, and W. R. Skowroneck. 1981. The role of superoxide and hydroxyl radical in the degradation of hyaluronic acid induced by metal ions and by ascorbic acid. J. Inorganic Biochem. 14, 127-134 https://doi.org/10.1016/S0162-0134(00)80033-1
  45. Yang, H. K., J. Y. Jin, J. M. Kim, M. S. Ko, H. J. Hong, S. C. Kim, and Y. J. Lee. 2006. Single oral dose toxicity study of the extract of Aralia elata in mice. J. Toxical. Pub. Health 22, 439-443
  46. Yoshikawa, M., T. Murakawa, E. Harada, N. Murakami, J. Yamahara, and H. Matsuda. 1996. Bioactive saponins and glycosides. VII. On the hypoglycemic principles from the root cortex of Aralia elata Seem.: structure related hypoglycemic activity of oleanolic acid oligoglycoside. Chem. Pharm. Bull (Tokyo) 44, 1923-1927 https://doi.org/10.1248/cpb.44.1923
  47. Yoshikawa, M., H. Matsuda, Harada, N. Murakami, J. Wamahara, and N. Murakami. 1994. Elatoside E, a new hypoglycemic principle from the root cortex of Aralia elata Seem.: structure-related hypoglycemic activity of oleanolic acid glycosides. Chem. Pharm. Bull (Tokyo) 42, 1354-1356 https://doi.org/10.1248/cpb.42.1354
  48. Yoo, J. H., J. Y. Cha, Y. K. Jeong, K. T. Chung, and Y. S. Cho. 2004. Antioxidative effects of pine (Pinus denstifora) needle extracts. J. Life Sci. 14, 863-867 https://doi.org/10.5352/JLS.2004.14.5.863

피인용 문헌

  1. Development of recipe for promoting consumption of Haetsun sprouts and their nutrient evaluation vol.21, pp.5, 2014, https://doi.org/10.11002/kjfp.2014.21.5.710
  2. Antioxidant Activity of Methanol Extracts from Lactuca indica vol.19, pp.2, 2012, https://doi.org/10.11002/kjfp.2012.19.2.294
  3. Antioxidant and antimicrobial activities of leafy green vegetable extracts and their applications to meat product preservation vol.29, pp.1, 2013, https://doi.org/10.1016/j.foodcont.2012.05.060
  4. Quality Characteristics of Noodles Containing Shoots and Leaves of Aralia elata and Kalopanax pictus vol.41, pp.4, 2012, https://doi.org/10.3746/jkfn.2012.41.4.571
  5. Quality Characteristics of Kalopanax pictus and Aralica elata Shoot according to Their Salt Conditions vol.19, pp.2, 2012, https://doi.org/10.11002/kjfp.2012.19.2.193
  6. Antioxidant Activities and Monacolin K Production of Fermented Chinese Yam by Monascus sp. MK805 vol.50, pp.1, 2014, https://doi.org/10.7845/kjm.2014.4002
  7. Antioxidative Activity and Chemical Characteristics of Leaf and Fruit Extracts from Thuja orientalis vol.21, pp.5, 2011, https://doi.org/10.5352/JLS.2011.21.5.746
  8. Chemical properties and antioxidant activities of the sprouts of Kalopanax pictus, Cedrela sinensis, Acanthopanax cortex at different plucking times vol.20, pp.3, 2013, https://doi.org/10.11002/kjfp.2013.20.3.356
  9. Biological Activity of Methanolic Extract from Ganoderma lucidum, Momordica charantia, Fagopyrum tataricum, and Their Mixtures vol.21, pp.7, 2011, https://doi.org/10.5352/JLS.2011.21.7.1016
  10. Antioxidative Activity and Chemical Characteristics of Leaves, Roots, Stems and Fruits Extracts from Acanthopanax senticosus vol.21, pp.7, 2011, https://doi.org/10.5352/JLS.2011.21.7.1052
  11. Biological Activities of Ethanol Extracts from Monascus-Fermented Chinese Yam vol.21, pp.8, 2011, https://doi.org/10.5352/JLS.2011.21.8.1142
  12. Inhibitory effect ofAralia elataethanol extract against skin damage in UVB-exposed human keratinocytes and human dermal fibroblasts vol.49, pp.6, 2016, https://doi.org/10.4163/jnh.2016.49.6.429
  13. Antioxidative Activities and Qualitative Characteristics of Substitute Tea using Salvia plebeia R. Br. vol.31, pp.1, 2015, https://doi.org/10.9724/kfcs.2015.31.1.041
  14. Biological Activity and Chemical Characteristics of Fermented Acanthopanax senticosus by Mold vol.22, pp.12, 2012, https://doi.org/10.5352/JLS.2012.22.12.1704
  15. Suppressive effects of ethanol extract ofAralia elataon UVB-induced oxidative stress in human keratinocytes vol.49, pp.3, 2016, https://doi.org/10.4163/jnh.2016.49.3.135
  16. The Antioxidative Properties of Ganghwayakssuk (Artemisia princeps Pamp.) Extracts Added to Refrigerated Raw Chicken Nugget Batter against Lipid Oxidation vol.31, pp.2, 2011, https://doi.org/10.5851/kosfa.2011.31.2.166
  17. Effect of extract temperature and duration on antioxidant activity and sensory characteristics of Ulmus pumila bark extract vol.23, pp.7, 2016, https://doi.org/10.11002/kjfp.2016.23.7.995