Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Effect of Exercise Training (EXE) on Myocardium Glucose Metabolic Phenotypic Proteins and HSP-60 Protein Expression after Ischemia/Reperfusion Injury in STZ-induced Rats

지구성 운동이 STZ-당뇨 유발 쥐의 허혈/재 관류 손상 후 심근의 당대사 관련 표현형 단백질과 HSP-60 단백질 발현에 미치는 영향

  • Bae, Hee-Suk (Department of Exercise Biochemistry Laboratory, Korea National Sport University) ;
  • Um, Hyun-Seob (Department of Exercise Biochemistry Laboratory, Korea National Sport University) ;
  • Kang, Eun-Bum (Department of Exercise Biochemistry Laboratory, Korea National Sport University) ;
  • Yang, Chum-Yeol (Department of Exercise Biochemistry Laboratory, Korea National Sport University) ;
  • Lee, Yong-Ro (Department of Exercise Biochemistry Laboratory, Korea National Sport University) ;
  • Lee, Chang-Guk (Department of Physical Education, Soon Chun Hyang University) ;
  • Cheon, U-Ho (Department of Physical Education, Soon Chun Hyang University) ;
  • Jeon, Hye-Ja (Department of Physical Education, Soon Chun Hyang University) ;
  • Cho, In-Ho (Department of Exercise Biochemistry Laboratory, Korea National Sport University) ;
  • Cho, Joon-Yong (Department of Exercise Biochemistry Laboratory, Korea National Sport University)
  • 배희숙 (한국체육대학교 체육과학관 운동생화학실) ;
  • 엄현섭 (한국체육대학교 체육과학관 운동생화학실) ;
  • 강은범 (한국체육대학교 체육과학관 운동생화학실) ;
  • 양춘열 (한국체육대학교 체육과학관 운동생화학실) ;
  • 이용로 (한국체육대학교 체육과학관 운동생화학실) ;
  • 이창국 (순천향대학교 체육학과) ;
  • 천우호 (순천향대학교 체육학과) ;
  • 전혜자 (순천향대학교 체육학과) ;
  • 조인호 (한국체육대학교 체육과학관 운동생화학실) ;
  • 조준용 (한국체육대학교 체육과학관 운동생화학실)
  • Published : 2009.05.30
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Abstract

The objective of this study was to identify EXE (1 hr a day at 21 m/min for 5 day/wk, at 0 % grade for 6 wk) on myocardium glucose metabolic phenotypic proteins (AMPK-PGC-1${\alpha}$-GLUT-4) and HSP-60 protein expression after ischemia/reperfusion injury (IRI) in STZ-induced rats. EXE was performed using STZ-induced diabetic rats on a rodent treadmill (28 m/min, 1 hr/day, 5 day/wk for 6 wk). The results of this study suggest that i) serum insulin level was not changed among groups (p>l0.05). ii) the LVDP level increased significantly in the STZ-EXE-IRI group compared to the STZ-IRI group at 60 min (p<0.01), 70 min (p<0.05) and 80 min (p<0.05) after reperfusion, respectively, and iii) AMPK phosphorylation (p<0.01), PGC-1${\alpha}$ protein (p<0.001), GLUT-4 protein (p<0.001) and HSP-60 protein expressions (p<0.05) increased significantly in the STZ-EXE-IRI group compared to the STZ-IRI group. In conclusion, the findings of the present study reveal that EXE may provide therapeutic value to insulin dependent diabetic patients with peripheral insulin resistance and myocardium injury by improving glucose metabolic proteins (AMPK-PGC-1${\alpha}$-GLUT-4) and heat shock protein-60 (HSP-60), along with increasing LVDP levels and decreasing glucose levels. Therefore, EXE protects the STZ-induced diabetic myocardium injury against ischemia/ reperfusion injury.

Keywords

References

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