대한한의학회지 (The Journal of Korean Medicine)

  • 제30권3호
  • /
  • Pages.51-60
  • /
  • 2009
  • /
  • 1010-0695(pISSN)
  • /
  • 2288-3339(eISSN)
대한한의학회 (The Society of Korean Medicine)
권호 표지

The Effect of Taglisodog-eum Extract on Lipopolysaccharide-induced Otitis media

  • Jung, Arumnuri (Dept. of Dermatology, Ophthalmology & Otorhinolaryngology, Hospital of Oriental Medicine, Dongguk University) ;
  • Hong, Seung-Ug (Dept. of Dermatology, Ophthalmology & Otorhinolaryngology, Hospital of Oriental Medicine, Dongguk University)
  • 발행 : 2009.05.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : It has been known that immune reaction plays an important role in the pathogenesis of otitis media (OM). We investigated the change of middle ear mucosal inflammation induced by lipopolysaccharide (LPS) following administration of Taglisodog-eum (TSE) in experimental animals. Materials and Methods : Otitis media was induced by injecting 1 mg/kg of Pseudomonas aeruginosa LPS transtympanically in 60 rats. These rats were divided into two groups, the LPS group (n=30, remained naive after OM elicitation) and the TSE group (n=30, treated with TSE after OM elicitation) and 6 additional rats were kept as a normal control group (n=6, remained naive until tissue collection). The rats were killed at the 1st, 3rd, and 7th days after challenge. The temporal bones in each group were harvested and examined histopathologically by hematoxyline-eosin stain. Middle ear mucosa were taken at the 1st, 3rd, and 7th days after challenge. The levels of spicing variants of TNF-${\alpha}$ transcription were evaluated by Realtime-PCR. Results : TSE suppressed LPS-induced TNF-${\alpha}$ mRNA expression and thickness of the submucosal layer and infiltration of inflammatory cells in rat middle ear epithelium. Conclusion : The results suggest that TSE may be effective in decreasing inflammation with particular application to mucosal metaplasia in OM.

키워드

참고문헌

  1. Barrett TQ, Kristiansen LH, Ovesen T. NFkappa B in cultivated middle ear epithelium. Int J Pediatr Otorhinolaryngol, 2003;67(8):895-903.
  2. Daly KA. Epidemiology of otitis media. Otolaryngol Clin North Am, 1991;24(4):775-86.
  3. Richard M, Rosenfeld LC, Karen JD, Kenneth MG, Alejandro H, Margaret AK, et al. Clinical practice guideline: Otitis media with effusion. Otolaryngology-Head and Neck Surgery, 2004; 130(5):95-118. https://doi.org/10.1016/j.otohns.2004.02.002
  4. Tos M, BP. The pathogenesis of chronic secretory otitis media. Arch Otolaryngol Head Neck Surg,1972;95:511-21. https://doi.org/10.1001/archotol.1972.00770080801004
  5. Maeda K. Cytokine expression in experi- mental chronic otitis media with effusion in mice. Laryngoscope, 2004;114(11):1967-72. https://doi.org/10.1097/01.mlg.0000147930.29261.51
  6. Ovesen T, Ledet T. Bacteria and endotoxin in middle ear fluid and the course of secretory otitis media. Clin Otolaryngol Allied Sci, 1992;17(6):531-4. https://doi.org/10.1111/j.1365-2273.1992.tb01713.x
  7. Jung MK. Role of Inflammatory Mediators in the Pathogenesis of Otitis Media. Korean J Otolaryngol,2007;50(1):2-13.
  8. Kim JH, Kim HD, Bae SH, Lee JK, Kim CG, Na AS, et al. Studies on Relationships between Inflammatory Cell, Cytokines and Clinical Features in Pediatric Otitis Media with Effusion. Korean J Otolaryngol, 2003;46(10):815-21.
  9. Lee EM, Park EJ. Otitis Media is one of the Most Common Disease of Otolaryngology and Pediatrics. J Korean Oriental Pediatrics, 1999;13(2): 149-70.
  10. Jo SH, Lee SG. A Clinical Report for Treatment 2 Cases on Takrisodokyeum of Otitis media. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology, 2002;15(1):301-7.
  11. Jo SH, Lim JH, Lee SG, Jee SY. Case of Otitis Media Treated with Sonbanghwalmyongum. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology,2003;16(1):198-205.
  12. Kim HH, Park EJ, Joo JC. Effect of Kamihyunggyeyungyotang on Interleukin-8 & TGF-$\beta$ in Middle Ear Effusion for Pediatric Recurrent Otitis Media with Effusion. J Korean Oriental Pediatrics, 2002;16(2):39-49.
  13. Hwang-bo M, Lim JH, Jee SY, Lee SK. A Clinical Report for 2 Cases on Hyenggaeyeonggyotanghab-bojungikgitang of Otitis Media. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology, 2004;17(3):146-52.
  14. Lee HJ, Park EJ. A Clinical Study on the Herbal Medicine Therapy Treating Pediatric Chronic Sinusitis and Otitis Media. J Korean Oriental Pediatrics, 1999;13(1):205-26.
  15. Kim GS, Kim DG, Lee JY. An Experimental Syudy on the Anti-allergic effect on the Taklisodok-um. J Korean Oriental Pediatrics, 1994;8(1):27-37.
  16. Seo HS. The effect of GamiTakliSodocyum on Wound Healing. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology, 2001;14(2):89-111.
  17. Kang HG, Kim YH, Kang HS, Kim HD, Ko WS. The Anti-Inflammatory Effect of Takrisodokeum in the Ing-Juh. J Korean Oriental Med 2000;21(1):45-52.
  18. Lee HJ. Apoptosis-inducing Effect of Taklisodokyeum Extract in Androgen Independent Prostate Cancer Cells. Graduate School of Wonkwang University, 2003.
  19. Choi W. The effect of TaklisodokSan extract on anti-tumor action and immune-function. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology, 1999;12(1):79-98.
  20. Kim MH, Lee JY, Lee JW, Cho CS, Kim CS. The Clinical Study in 20 Cases of Patients with Chronic Prostatitis on the effects by Taklisodokeum for 1 month. Daejon University, 2002;11(1):103-10.
  21. Skotnicka B, Hassmann E. Cytokines in children with otitis media with effusion. Eur Arch Otorhinolaryngol, 2000;257(6):323-6. https://doi.org/10.1007/s004059900218
  22. Hotomi M, Samukawa T, Yamanaka N. Interleukin-8 in otitis media with effusion. Acta Otolaryngol,1994;114(4):406-9. https://doi.org/10.3109/00016489409126078
  23. Johnson MD. Cytokines in experimental otitis media with effusion. Laryngoscope, 1994;104(2):191-6.
  24. Jeon EJ, Park YS, Choi Y, Lee JH. Effect of Inhibitor of TNF-$\alpha$ on Lipopolysaccharide-induced Otitis Media with Effusion. Korean J Otolaryngol,2000;43:1040-4.
  25. Albiin N. Middle ear mucosa in rats and humans. Ann Otol Rhinol Laryngol Suppl, 1986;126:2-15.
  26. Park SN. The Effects of Antibiotics and Steroid on the Middle Ear Mucosa in the Rats with Experimental Acute Otitis Media. Korean J Otolaryngol, 2000;43(3):249-55.
  27. Rhee CK. Effect of Platelet-Activating Factor and PAF-Antagonist on the Mucociliary Clearance of the Eustachian Tube in Guinea Pigs. Korean J Otolaryngol, 1999;42:273-8.
  28. Lee DH, Choi YC, Jeon EJ, Yeo SW. Effect of Tumor Necrosis Factor-$\alpha$ on Experimental Otitis Media with Effusion. Korean J Otolaryngol,2000;43(9):918-94.
  29. Kim BG, Jung DG, Park SY, Yeo SW, Chae SY. Experimental Otitis Media with Effusion Induced by Interleukin 2. Korean J Otolaryngol, 2001;42(21-5).
  30. DeMaria TF, Murwin DM. Tumor necrosis factor during experimental lipopolysaccharide- induced otitis media. Laryngoscope, 1997;107(3):369-72. https://doi.org/10.1097/00005537-199703000-00017
  31. Ball SS. Role of tumor necrosis factor and interleukin-1 in endotoxin-induced middle ear effusions. Ann Otol Rhinol Laryngol, 1997;106(8):633-9. https://doi.org/10.1177/000348949710600803
  32. Iino Y, Kaneko Y, Takasaka T. Endotoxin in middle ear effusions tested with Limulus assay. Acta Otolaryngol, 1985;100(1-2):42-50. https://doi.org/10.3109/00016488509108586
  33. Le J, Vilcek J. Tumor necrosis factor and interleukin 1: cytokines with multiple overlapping biological activities. Lab Invest, 1987;56(3):234-48.
  34. Pennica D. Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin. Nature, 1984;312(5996):724-9. https://doi.org/10.1038/312724a0
  35. Maxwell KS. Interleukin-8 expression in otitis media. Laryngoscope, 1994;104(8 Pt 1): 989-95.
  36. Brodsky L. Arachidonic acid metabolites in middle ear effusions of children. Ann Otol Rhinol Laryngol, 1991;100(7):589-92. https://doi.org/10.1177/000348949110000714
  37. Moon SK. Synergistic effect of interleukin 1 alpha on nontypeable Haemophilus influenzaeinduced up-regulation of human beta-defensin 2 in middle ear epithelial cells. BMC Infect Dis,2006;6:12. https://doi.org/10.1186/1471-2334-6-12