한국해양환경ㆍ에너지학회지 (Journal of the Korean Society for Marine Environment & Energy)

  • 제12권2호
  • /
  • Pages.91-95
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  • 2009
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  • 2288-0089(pISSN)
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  • 2288-081X(eISSN)
한국해양환경•에너지학회 (The Korean Society for Marine Environment and Energy)
권호 표지

실내 배양시 부착기질 크기에 따른 저서성 미세조류 Nitzschia sp.의 성장 특성

Effect of Attachment Substrate Size on the Growth of a Benthic Microalgae Nitzschia sp. in Culture Condition

  • 오석진 (부경 대학교 해양과학공동연구소) ;
  • 윤양호 (전남대학교 수산해양대학 해양기술학부) ;
  • 산본민차 (히로시마대학 대학원 생물권과학연구과) ;
  • 양한섭 (부경 대학교 해양과학공동연구소)
  • Oh, Seok-Jin (Korea Inter-University Institute of Ocean Science, Pukyong National University) ;
  • Yoon, Yang-Ho (Faculty of Marine Technology, Chonnam National University) ;
  • Yamamoto, Tamiji (Graduate School of Biosphere Science, Hiroshima University) ;
  • Yang, Han-Soeb (Korea Inter-University Institute of Ocean Science, Pukyong National University)
  • 발행 : 2009.05.25
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초록

저서성 미세조류의 성장에 미치는 부착기질의 영향을 알기 위해 서로 다른 크기의 glass bead를 첨가하여 저서성 미세조류 Nitzschia sp.(진해만 클론)의 성장을 조사하였다. 연구에 사용된 glass bead의 크기는 0.09-0.15 mm(G1), 0.25-0.50 mm(G2), 0.75-1.00 mm(G3) 그리고 1.25-1.65 mm(G4)이며, glass bead를 첨가하지 않는 대조구도 설정하였다. Nitzschia sp.의 가장 높은 성장속도(0.37/day)와 최대세포밀도($9,232{\pm}840$ cells/mL)는 가장 작은 크기의 glass bead를 첨가한 G1에서 나타났다. 그리고 성징속도와 최대세포밀도는 glass bead 크기의 증가와 함께 감소하였다(G4의 성장속도와 최대세포밀도는 각각 0.24/day와 $6,397{\pm}524$ cells/mL였다). 더욱이 대조구의 성장속도는 실험구 G1에서 G3의 성장속도보다 상당히 낮았다. 이 결과에서 Nitzschia sp.와 같은 저서성 미세조류를 위한 부착기질은 성장속도 뿐만 아니라 세포밀도에도 큰 영향을 주는 것으로 나타났다. 따라서 부착미세조류의 생리실험을 위해서는 예비실험으로 부착기질의 유무 및 대상 종에 적합한 부착입자의 크기의 고려가 필요할 것으로 보인다.

To understand the effect of attachment substrate on the growth of benthic microalgae, we experimentally investigated the growth of benthic microalgae Nitzschia sp. (Jinhae Bay strain) with additions of glass beads in different sizes. The glass beads used in this study are 0.09-0.15 mm (G1), 0.25-0.50 mm (G2), 0.75-1.00 mm (G3) and 1.25-1.65 mm (G4). No addition of glass beads used as controls. Highest specific growth rate (0.37/day) and maximum cell density ($9,232{\pm}840$ cells/mL) of Nitzschia sp. showed at the smallest glass beads (G1), and the specific growth rate and maximum cell density were decreasing with increasing size of glass beads (specific growth rate and maximum cell density of G4 was 0.24/day and $6,397{\pm}524$ cells/mL, respectively). Moreover, specific growth rate of the control experiment (0.23/day) was significantly lower than their of G1 to G3 experiment. The results indicated that the attachment substrate for benthic microalgae as Nitzschia sp. is important factor which affecting the growth rate as well as cell density. Therefore, the physiological experiment of benthic microalgae seems to be necessary to preliminary experiment, which is addition or not of the attachment suitable substrate and the grain size for the target species of benthic microalgae.

키워드

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