Clinical and Experimental Reproductive Medicine

  • 제36권2호
  • /
  • Pages.101-109
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  • 2009
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

소의 초기 배 발생단계별 Telomeric DNA 함량 및 Telomerase Activity 분석

Telomeric Dynamics and Telomerase Activity in Early Bovine Embryos

  • 정예화 (진주산업대학교 동물생명과학과) ;
  • 이수희 (진주산업대학교 동물생명과학과) ;
  • 조상래 (국립축산과학원 가축유전자원시험장) ;
  • 공일근 (경상대학교 응용생명과학부) ;
  • 조재동 (엘르메디산부인과) ;
  • 손시환 (진주산업대학교 동물생명과학과)
  • Jung, Yei-Hwa (Department of Animal Science & Biotechnology, Jinju National University) ;
  • Lee, Soo-Hee (Department of Animal Science & Biotechnology, Jinju National University) ;
  • Cho, Sang-Rae (Animal Genetic Resources Station, National Institute of Animal Science) ;
  • Kong, Il-Keun (Division of Applied Life Science (BK21 Program), Gyeongsang National University) ;
  • Cho, Jae-Dong (Ellemedi Obstetrics and Gynecology) ;
  • Sohn, Sea-Hwan (Department of Animal Science & Biotechnology, Jinju National University)
  • 발행 : 2009.06.30
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초록

목 적: 소 수정란의 telomeric DNA 함량 및 텔로머레이스 활성도 (telomerase activity)를 분석하여 초기 배아형성과정에서 텔로미어의 변화 양상을 규명하고자 하였다. 연구방법: 소의 초기 배 발생단계별 체외수정란을 대상으로 양적형광보인법 (Q-FISH)을 이용한 telomeric DNA의 함량 분석과 Telomeric Repeat Amplification Protocol (TRAP)로서 텔로머레이스 활성도를 분석하였다. 결 과: 소의 초기 수정란의 telomeric DNA 함유율은 발생과정이 진행됨에 따라 유의적인 양적 증가를 나타내었다. 배반포배의 질 (quality)에 따른 텔로미어의 함량 차이는 나타나지 않았다. 소 수정란의 8 세포기, 상실배기 및 배반포기의 모든 초기 배 단계에서 텔로머레이스 활성도를 보였으며, 상실배에서 증가하여 배반포배에서 가장 강한 활성도를 나타내었다. 결 론: 소의 초기 배아형성과정이 진행되면서 텔로미어의 함량 및 텔로머레이스 활성도는 점진적으로 증가하고 이들 간에는 정 (positive)의 상관관계를 가진다. Telomeric DNA 함량의 증가를 유지하기 위하여 텔로머레이스의 활성화는 필수적인 것으로 보여진다. 배반포기 배아에서 높은 텔로미어 함량과 강한 텔로머레이스 활성도는 이 시기가 배발생 및 분화과정 중 가장 세포적 활성도가 높은 시점임을 의미하는 것으로 사료된다.

Objective: This study was carried out to analyze the amount of telomeric DNA and telomerase activity in early bovine embryos. Methods: The amount of telomeric DNA in early bovine embryos at the 8 cell, morula and blastocyst stages was analyzed by Quantitative Fluorescence In Situ Hybridization (Q-FISH) technique using a bovine telomeric DNA probe. Telomerase activity was analyzed by Telomeric Repeat Amplification Protocol (TRAP assay). Results: The relative amount of telomeric DNA in early bovine embryos was gradually increased from 8 cell to blastocyst stage. It was not significantly associated with the grade of embryo quality. While telomerase activity was detected in the early bovine embryos at these stages, it significantly increased at morula stage and showed maximum activity at the blastocyst stage. Conclusion: The amount of telomeric DNA and the telomerase activity of bovine embryos increase during the progression of early embryogenesis, suggesting a positive correlation between telomeric DNA and telomerase activity. The telomerase activity seems to increase to maintain the levels of telomeric DNA through embryo development which are required for extensive cell division.

키워드

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