Abstract
Objective: The aim of this study was to compare the fertilization and cleavage rates of human in vitro matured oocytes after fertilized by conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Methods: A total of 135 GV stage oocytes were obtained from 59 women who received ovarian stimulation and IVF during Jan 2007 to Oct 2008. Ovarian hyperstimulation was performed using hMG or recombinant FSH with GnRH antagonist and then ovulation triggered by recombinant hCG. The immature oocytes obtained from stimulation cycles were cultured in IVM medium up to 48 hrs; commercial medium supplemented with rFSH 75 mIU/mL, rhCG 0.5 IU/mL and rEGF 10 ng/mL. The in vitro matured oocytes were fertilized by conventional IVF (41 GV oocytes) or ICSI method (94 GV oocytes). Results: Maturation rate were 51.2% and 59.6% in conventional IVF group and ICSI group, respectively. There was no significant difference in fertilization rates between two groups; 71.4% and 80.4%, respectively. The cleavage rate was also similar in two groups. Conclusion: The presented data suggest that conventional IVF has comparable fertilization and cleavage potential compared with ICSI as the insemination method of immature human oocytes obtained from stimulated cycle.
목 적: 고식적 체외수정시술과 세포질내정자주입법을 사용하여 체외성숙 난자를 수정시키고 수정률과 배발달율을 비교하고자 하였다. 연구방법: 2007년 1월부터 2008년 8월까지 난소과자극과 체외수정시술을 받은 59명의 여성에서 135개의 배아소포 단계의 미성숙난자만을 얻어 75 mIU/mL rFSH, 0.5 IU/mL rhCG, 10 ng/mL rEGF가 포함된 체외성숙용 배양액에서 최대 48시간까지 배양하였다. 체외성숙된 난자는 고식적 체외수정방법 (n=41) 또는 세포질내정자주입법 (n=94)을 이용하여 수정시켰으며 이후 배발달율을 관찰하였다. 결 과: 고식적 체외수정군과 세포질내정자주입군에서 체외성숙률은 각각 51.2%와 59.6%였고, 수정률은 각각 71.4%와 80.4%로 두 군간에 통계적으로 유의한 차이는 없었다. 배발달율도 두 군에서 비슷하게 관찰되었다. 결 론: 난소과자극을 통해 얻은 미성숙난자는 고식적 체외수정법을 사용하더라도 세포질내정자주입법과 동등한 수정률과 배발달율을 보임을 확인하였다.