Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Oligonucleotide Array-based Detection and Genotyping of Mollicutes (Acholeplasma, Mycoplasma, and Ureaplasma)

  • Received : 2008.06.02
  • Accepted : 2008.07.24
  • Published : 2009.03.31
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Abstract

An oligonucleotide array was developed to detect and genotype mollicutes based on the internal transcribed spacer (ITS) sequence. The results of the assay were compared with those of a PCR-RFLP assay. The proposed oligonucleotide array containing 5 genus- and 23 species-specific probes was able to detect Mycoplasma species, including M. penetrans and M. spermatophilum, that were not detected by the PCR-RFLP assay. Therefore, the results demonstrated that the proposed oligonucleotide array was effective for the detection and discrimination of 23 species, including an acholeplasma, 21 mycoplasmas, and a ureaplasma, and showed promise as a countermeasure to ensure that biological products are safe and of good quality.

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References

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Cited by

  1. [Letter to the editor] Unexpected presence of mycoplasma probes on human microarrays vol.47, pp.6, 2009, https://doi.org/10.2144/000113271
  2. Mycoplasma testing of cell substrates and biologics: Review of alternative non-microbiological techniques vol.25, pp.2, 2009, https://doi.org/10.1016/j.mcp.2011.01.002