Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Clean-up of the Crude Oil Contaminated Marine Sediments Through Biocarrier-Mediated Bioaugmentation

생물담체 활용 생물접종에 의한 원유로 오염된 해양토양의 정화

  • Ekpeghere, Kelvin I. (Division of Civil and Env. Eng., Korea Maritime University) ;
  • Bae, Hwan-Jin (Department of Marine Env. Eng. and Institute of Marine Industry, Gyeongsang National University) ;
  • Kwon, Sung-Hyun (Department of Marine Env. Eng. and Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Byung-Hyuk (Environ. Bio-Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Duck-Ja (Eulji Entech, Inc.) ;
  • Kim, Hee-Shik (Environ. Bio-Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Koh, Sung-Cheol (Division of Civil and Env. Eng., Korea Maritime University)
  • ;
  • 배환진 (경상대학교 해양환경공학과 및 해양산업연구소) ;
  • 권성현 (경상대학교 해양환경공학과 및 해양산업연구소) ;
  • 김병혁 (한국생명공학연구원 환경바이오센터) ;
  • 박득자 (을지엔텍(주)) ;
  • 김희식 (한국생명공학연구원 환경바이오센터) ;
  • 고성철 (한국해양학교 건설환경공학부)
  • Received : 2009.12.08
  • Accepted : 2009.12.18
  • Published : 2009.12.31
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Abstract

This study was carried out to develop an effective biocarrier-mediated bioaugmentation technology which will be useful for remediation of the crude oil-contaminated marine sediments. Enrichment of several microbial communities was made from several oil-polluted seashore sites and the two distinctively functional consortia have been successfully selected. These two consortia were grown together and used to manufacture the microbial agents for bioaugmentation of marine sediments polluted with crude oil. The most dominant species in the mixed culture was identified as Alcanivorax borkumensis based on pure culture and DGGE analysis. Bioaugmentation of oil-polluted marine sediments with the microbial agent MA-2 formulated using the mixed culture and biocarriers (activated carbon and minerals) was more effective, especially in combination with an oxygen producing (releasing) compound (ORC). Ninty percent of TPH was removed in the presence of ORC in 35 days while 74% in the absence of ORC. This indicated that the indigenous consortial degraders could be immobilized on the active carbon as a biocarrier to manufacture microbial agents and then effectively bioaugmented for remediation of the oil-polluted sediments.

본 연구의 목표는 생물담체(biocarrier)에 의한 생물접종기술(bioaugmentation)을 개발하여 원유로 오염된 해양저질의 정화에 활용하고자 하는 것이다. 몇 군데의 원유로 오염된 해안으로부터 수 가지의 분해미생물군집을 농화배양하여 평가한 결과 기능적으로 상이한 2가지의 미생물군집을 분리하였다. 이들 미생물군집을 혼합 배양한 경우 Alcanivorax sp.가 우점종을 이루는 것으로 나타났으며, 이 군집과 대나무활성탄 등을 이용하여 미생물제제(MA-2)를 제조하여 사질의 원유오염 해안토양에 처리할 경우 5주 후 산소발생제의 존재하에 90% 이상의 TPH 분해력을 나타내었다. 또한 점질의 토양도 미생물제제(MA-1)를 처리할 경우 5주 후 71% 정도의 분해율을 나타냈다. 이는 분리된 토착미생물군집을 활용하여 오염토양의 처리에 효과적으로 활용할 수 있음을 의미한다. 한편 계면활성제의 고농도의 처리는 분해미생물의 작용을 억제하므로 적절한 농도의 확인이 필요하며 점토질의 토양의 정화를 위해서는 적절한 통기를 시키는 방법(산소발생제 투여, 기계적 aeration 등)의 활용이 요구된다.

Keywords

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