Textile Science and Engineering (한국섬유공학회지)

The Korean Fiber Society (한국섬유공학회)
권호 표지

Degradation of Hemp Lignin and Pectin by Microorganism

미생물을 이용한 대마의 리그닌과 펙틴 분해

  • Kim, Hyung-Joo (Department of Microbial Engineering, College of Engineering, Konkuk University) ;
  • Kwon, Kil-Koang (Department of Microbial Engineering, College of Engineering, Konkuk University) ;
  • Seo, Kyu-Won (Department of Microbial Engineering, College of Engineering, Konkuk University) ;
  • Kim, Min-Chul (HempLee Korea) ;
  • Kim, Hyung-Sup (Department of Textile Engineering, College of Engineering, Konkuk University) ;
  • Koh, Joon-Seok (Department of Textile Engineering, College of Engineering, Konkuk University)
  • Received : 2009.08.25
  • Accepted : 2009.11.23
  • Published : 2009.12.31
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Abstract

In this study, microbial degradation of lignin and pectin in raw hemp was investigated. Using lignin and pectin containing media, various microorganisms were isolated from soil and fecal samples of ruminant. The isolates were identified using the 16s rRNA method. The isolated strains were Klebsiella sp., E. coli, Aspergillus fumigatus, and Burkholderia sp. For the degradation of hemp, a pre-cultured isolate was inoculated (5% v/v) into the medium containing raw hemp (as a sole carbon source: 1% w/v) and nitrogen sources and the mixture was incubate aerobically for 168 hours in a shaking incubator (200 rev $min^{-1}$) at $30^{\circ}C$. The hemp biodegradation activity of the identified microorganism was measured by FT-IR. The FT-IR results showed that the Klebsiella sp. has the highest lignin and pectin degradation activity. These results show that the isolates and the reaction conditions could be applied to the conventional hemp fiber refinery processes.

Keywords

Acknowledgement

Supported by : 지식경제부, 한국산업기술진흥원

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