Journal of Microbiology and Biotechnology

  • 제19권4호
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  • Pages.424-430
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  • 2009
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Role of IL-10 Deficiency in Pneumonia Induced by Corynebacterium kutscheri in Mice

  • Jeong, Eui-Suk (College of Veterinary Medicine, College of Science, Konkuk University) ;
  • Won, Young-Suk (Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Hyoung-Chin (Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Myung-Hawn (Department of Biological Sciences, College of Science, Konkuk University) ;
  • Choi, Yang-Kyu (College of Veterinary Medicine, College of Science, Konkuk University)
  • 발행 : 2009.04.30
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초록

IL-10 is an important anti-inflammatory cytokine that can inhibit the production of many pro-inflammatory cytokines. Both human and animal studies have shown that pro-inflammatory cytokines play an important role in pneumonia and other inflammatory lung diseases. In the present study, IL-10 knockout(KO) and wild-type mice were infected with Corynebacterium kutscheri to determine whether the severity of pathogenesis and whether protective immunity could be altered in the absence of IL-10. The survival rate was significantly lower in IL-10 KO mice than wild-type mice. The number of neutrophils in bronchoalveolar lavage fluid and blood were found to be higher in IL-10 KO mice than wild-type mice. IL-10 KO mice showed greater neutrophil infiltration, excessive inflammation, and weight-loss compared with wild-type mice. Furthermore, upregulation of IFN-$\gamma$ in bronchoalveolar lavage fluid, and upregulation of MIP-$1{\alpha}$ and IP-10 mRNA in the lungs of IL-10 KO mice compared with wild-type mice after C. kutscheri infection were observed. These results suggest that IL-10 plays an important role in the anti-inflammatory properties against C. kutscheri infection, and that lack of IL-10 leads to a more severe pulmonary inflammatory response. This increased susceptibility to C. kutscheri pneumonia is at least in part caused by IL-10 deficiency and severe recruitment of neutrophils.

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참고문헌

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