Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of a Theta-Type Cryptic Plasmid from Bifidobacterium longum FI10564

  • Moon, Gi-Seong (Division of Food and Biotechnology, Chungju National University) ;
  • Wegmann, Udo (Commensals and Microflora(G2), Institute of Food Research, Norwich Research Park) ;
  • Gunning, A. Patrick (Imaging(IMG), Institute of Food Research, Norwich Research Park) ;
  • Gasson, Michael J. (Commensals and Microflora(G2), Institute of Food Research, Norwich Research Park) ;
  • Narbad, Arjan (Commensals and Microflora(G2), Institute of Food Research, Norwich Research Park)
  • Published : 2009.04.30
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Abstract

A number of bifidobacterial species of human origin were screened for the presence of cryptic plasmids. One strain, Bifidobacterium longum FI10564, harbored plasmids of approximately 2.2 kb, 3.6 kb, and 4.9 kb in size. The smallest plasmid, pFI2576(2,197 bp), was studied in detail and its complete nucleotide sequence was determined. Computer-assisted analysis of this novel plasmid(G+C content 62%) identified 9 putative open reading frames(orfs), 3 of which were shown to be probable genes. These putative genes are arranged in an operon-like structure, in which the overlapping orfs 1 and 2 encode putative Rep proteins and are highly homologous to the rep genes of the B. longum plasmid pMBI(1,847 bp). The mechanism of replication of pFI2576 was investigated using Southern blot analysis of whole cell lysates, with and without S1 nuclease treatment, and atomic force microscopy(AFM). The results indicate that pFI2576 is likely to use the theta mode of replication.

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References

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