Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Serotype and antimicrobial susceptibility of Salmonella spp. isolated from pigs and cattle

소와 돼지유래 Salmonella속 균의 혈청형 및 약제감수성

  • Lee, Woo-Won (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Jung, Byeong-Yeal (Nationa1 Veterinary Research & Quarantine Service) ;
  • Lee, Gang-Rok (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Lee, Dong-Soo (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Kim, Yong-Hwan (College of Veterinary Medicine, Gyeongsang National University)
  • 이우원 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 정병열 (국립수의과학검역원) ;
  • 이강록 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 이동수 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 김용환 (경상대학교 수의과대학)
  • Published : 2009.03.30
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Abstract

At the present study, it was aimed to explore the states of antimicrobial resistant Salmonella spp. isolates from 3,850 pigs (2,732 ileocecocolic lymphnodes and 1,118 cecal contents) and 1,764 cattle (965 cecal lymphnodes and 799 cecal contents) slaughtered in Busan province from December 2000 to November 2001. Among 5,614 samples, 457 of Salmonella spp. were isolated from pig lymphnodes (13.5%), pig cecal contents (4.4%), cattle lymphnodes (3.5%) and cattle cecal contents (0.5%). Salmonella spp. were showed different isolation ratio, that was 10.8% in summer, 9.0% in autumn, 8.4% in spring and 5.0% in winter. As a result of serotyping, B group (65.4%) were identified as the most common in pigs and cattle, in order of $C_1$ (14.0%), $D_1$ (5.5%), $C_2$ (4.2%), $E_1$ (4.2%) and L (3.5%). 34 serotypes were found, among them, Salmonella Typhimurium (S. Typhimurium) (21.0%) was the most common serotype from pigs and cattle. The major serotypes were in order of S. Derby (15.3%), S. Schwarzengrund (14.7%), S. Typhimurium var Copenhagen (9.2%), S. Mbandaka (5.7%), S. Enteritidis (5.5%) and S. Ruiru (3.5%). The most common serotype was S. Typhimurium in pigs, and S. Ruiru in cattle. S. Ruiru was firstly isolated from pigs and cattle in Korea. In antimicrobial susceptibility test, all the isolates were demonstrated susceptibility to norfloxacin and ofloxacin. But the isolates were showed resistance other antibiotics in order of doxycycline (68.3%), tetracycline (67.8%), penicillin (54.5%) and streptomycin (52.5%). S. Typhimurium were exhibited resistance to ampicillin (34.8%), chloramphenicol (36.2%), streptomycin (94.9%), sulfamethoxazole/trimethoprim (34.8%) and tetracycline (97.8%). There were 53 strains (38.4%) which had multi drug resistant (MDR) isolates, resistant to more than 6 antimicrobial agents. The most common resistance patterns of MDR isolates were ampicillin, chloramphenicol, carbenicillin, doxycycline, nalidixic acid, penicillin, streptomycin, sulfamethoxazole/trimethoprim and tetracycline (ACCbDNaPSSuT).

Keywords

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