Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Genetic sequence analysis of Porcine epidemic diarrhea virus (PEDV) detected from postweaning pigs in Korea

한국 이유자돈에서 검출된 돼지 유행성 설사 바이러스의 유전자 서열 분석

  • Shin, Hyun-Geun (Department of Veterinary Medicine and Institution of Veterinary Science, Kangwon National University) ;
  • Kim, Yeong-Hun (Department of Veterinary Medicine and Institution of Veterinary Science, Kangwon National University) ;
  • Seo, Tae-Won (Department of Veterinary Medicine and Institution of Veterinary Science, Kangwon National University) ;
  • Han, Jeong-Hee (Department of Veterinary Medicine and Institution of Veterinary Science, Kangwon National University)
  • 신현근 (강원대학교 수의학부(대학)) ;
  • 김영훈 (강원대학교 수의학부(대학)) ;
  • 서태원 (강원대학교 수의학부(대학)) ;
  • 한정희 (강원대학교 수의학부(대학))
  • Published : 2009.03.30
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Abstract

Porcine epidemic diarrhea virus (PEDV), an enveloped single stranded RNA virus in the family Coronaviridae, causes acute viral enteric disease in piglets. Recently outbreaks of porcine epidemic diarrhea (PED) have been rare in Europe but frequent in Asia. In Korea, the increase of PED prevalence is showing specially in postweaning pigs. The purpose of this study was to investigate nucleotide sequence of nucleocapsid protein gene of PEDV field isolates from postweaning pigs in Korea and get more information about the viruses. A total of 15 postweaing pigs clinically suspected of PEDV infection by severe watery diarrhea and dehydration were used in this study. Viral RNA was extracted from small intestines and stools of the pigs. The N gene was amplified by nested RT-PCR, purificated, sequenced, analyzed and then compared with published sequences of other PEDV strains. Three PEDVs were isolated from the suspected postweaning pigs. The N gene of three PEDV field isolates consisted of 483 nucleotides. These PEDV field isolates showed nucleotide sequence homology range from 99.6% to 95% with Chinese strains, from 99.8% to 95.2% with Korean strains, from 97.3% to 95.7% with Japanese strains and from 96.5% to 95.7% with Belgium and British strains. The encoded pritein shared range from 98.8% to 95.6% with Chinese strains, from 99.4% to 95% with Korean strains, from 97.5% to 96.3% with Japanese strains, from 95.6% to 95% with Belgium and British strains. By phylogenetic tree analysis based on nucleotide sequence, three PEDV field isolates were clustered into two groups which were Chinese isolate groups and other Korean isolate groups. These results indicated that some of PEDV field isolates prevailing in Korean postweaning pigs may be associated with those of Chinese strains and other Korean strains.

Keywords

References

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