Food Science and Biotechnology

  • 제18권2호
  • /
  • Pages.552-555
  • /
  • 2009
  • /
  • 1226-7708(pISSN)
  • /
  • 2092-6456(eISSN)
한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

Dandelion (Taraxacum officinale) Flower Ethanol Extract Inhibits Cell Proliferation and Induces Apoptosis in Human Ovarian Cancer SK-OV-3 Cells

  • Choi, Eun-Jeong (Plant Resources Research Institute, Duksung Women's University) ;
  • Kim, Gun-Hee (Plant Resources Research Institute, Duksung Women's University)
  • 발행 : 2009.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study investigated the proapoptotic effect of ethanol extracts obtained from dandelion (Taraxacum officinale) flower on human ovarian cancer SK-OV-3 cells. Cells were treated with dandelion flowers ethanol extract (DFE) ranging from 1.5625 to $100{\mu}g/mL$ for 24 hr. Significant antiproliferative effects of DFE were first observed from at $6.25{\mu}g/mL$ (p<0.05), and this inhibition showed in a dose-dependent manner. When cells were treated with more than $6.25{\mu}g/mL$ DFE, cell-cycle analysis showed that DFE caused an increase in the percentage of sub-G0/G1 cells and arrested at the S and G2/M phase in a dose-dependent manner. Moreover, apoptosis induction by DFE involved p53 activation and bax upregulation as well as downregulation of bcl-2. Our findings indicate that DFE resulted in apoptotic cell death, suggesting that DFE possesses potential anticancer properties.

키워드

참고문헌

  1. Kummalue T. Molecular mechanism of herbs in human lung cancer cells. J. Med. Assoc. Thai. 88: 1725-1734 (2005)
  2. Taixiang W, Munro AJ, Guanjian L. Chinese medical herbs for chemotherapy side effects in colorectal cancer patients. Cochrane Database Syst. Rev. 25: CD004540 (2005) https://doi.org/10.1002/14651858.CD004540.pub2
  3. Ahn DK. Illustrated Book of Korean Medical Herbs. Kyohak Publishing Co., Seoul, Korea. pp. 134-135 (1998)
  4. Koo HN, Hong SH, Song BK, Kim CH, Yoo YH, Kim HM. Taraxacum officinale induces cytotoxicity through TNF-alpha and IL-1alpha secretion in Hep G2 cells. Life Sci. 74: 1149-1157 (2004) https://doi.org/10.1016/j.lfs.2003.07.030
  5. Schutz K, Kammerer DR, Carle R, Schieber A. Characterization of phenolic acids and flavonoids in dandelion (Taraxacum officinale WEB. ex WIGG.) root and herb by high-performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Commun. Mass. Sp. 19: 179-186 (2005) https://doi.org/10.1002/rcm.1767
  6. Guarrera PM. Traditional phytotherapy in Central Italy (Marche, Abruzzo, and Latium). Fitoterapia 76: 1-25 (2005) https://doi.org/10.1016/j.fitote.2004.09.006
  7. Rodriguez-Fragoso L, Reyes-Esparza J, Burchiel SW, Herrera-Ruiz D, Torres E. Risks and benefits of commonly used herbal medicines in Mexico. Toxicol. Appl. Pharm. 227: 125-135 (2008) https://doi.org/10.1016/j.taap.2007.10.005
  8. Hu C, Kitts DD. Luteolin and luteolin-7-O-glucoside from dandelion flower suppress iNOS and COX-2 in RAW264.7 cells. Mol. Cell. Biochem. 265: 107-113 (2004) https://doi.org/10.1023/B:MCBI.0000044364.73144.fe
  9. Hu C, Kitts DD. Dandelion (Taraxacum officinale) flower extract suppresses both reactive oxygen species and nitric oxide and prevents lipid oxidation in vitro. Phytomedicine 12: 588-597 (2005) https://doi.org/10.1016/j.phymed.2003.12.012
  10. Baba K, Abe S, Mizuno D. Antitumor activity of hot water extract of dandelion, Taraxacum officinale-correlation between antitumor activity and timing of administration. Yakuga. Zasshi 101: 538-543 (1981) https://doi.org/10.1248/yakushi1947.101.6_538
  11. Sigstedt SC, Hooten CJ, Callewaert MC, Jenkins AR, Romero AE, Pullin MJ, Kornienko A, Lowrey TK, Slambrouck SV, Steelant WF. Evaluation of aqueous extracts of Taraxacum officinale on growth and invasion of breast and prostate cancer cells. Int. J. Oncol. 32:1085-1090 (2008)
  12. Le Marchand L. Cancer preventive effects of flavonoids-a review. Biomed. Pharmacotherapy 56: 296-301 (2002) https://doi.org/10.1016/S0753-3322(02)00186-5
  13. Ren W, Qiao Z, Wang H, Zhu L, Zhang L. Flavonoids: Promising anticancer agents. Med. Res. Rev. 23: 519-534 (2003) https://doi.org/10.1002/med.10033
  14. Kandaswami C, Lee LT, Lee PP, Hwang JJ, Ke FC, Huang YT, Lee MT. The antitumor activities of flavonoids. In Vivo 19: 895-909 (2005)
  15. Sun SY, Hail N Jr, Lotan R. Apoptosis as a novel target for cancer chemoprevention. J. Natl. Cancer Inst. 5: 662-672 (2004)
  16. Fesik SW. Promoting apoptosis as a strategy for cancer drug discovery. Nat. Rev. Cancer 5: 876-885 (2005) https://doi.org/10.1038/nrc1736
  17. Benavente-García O, Castillo J. Update on uses and properties of citrus flavonoids: New findings in anticancer, cardiovascular, and anti-inflammatory activity. J. Agr. Food Chem. 56: 6185-6205 (2008) https://doi.org/10.1021/jf8006568
  18. Jones J, Lagasse LD, Karlan BY. Steroid hormonal independence of HER-2/neu mRNA expression in four human ovarian carcinoma cell lines. Gynecol. Oncol. 53: 421-426 (1994)
  19. Lau KM, Mok SC, Ho SM. Expression of human estrogen receptoralpha and -beta, progesterone receptor, and androgen receptor mRNA in normal and malignant ovarian epithelial cells. P. Natl. Acad. Sci. USA 96: 5722-5727 (1999) https://doi.org/10.1073/pnas.96.10.5722
  20. Agarwal ML, Agarwal A, Taylor WR, Stark GR. p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts. P. Natl. Acad. Sci. USA 92:8493-8497 (1995) https://doi.org/10.1073/pnas.92.18.8493
  21. Park M, Chae HD, Yun J, Jung M, Kim YS, Kim SH, Han MH, Shin DY. Constitutive activation of cyclin B1-associated cdc2 kinase overrides p53-mediated G2-M arrest. Cancer Res. 60: 542-545 (2000)
  22. Lepik D, Jaks V, Kadaja L, Varv S, Maimets T. Electroporation and carrier DNA cause p53 activation, cell cycle arrest, and apoptosis. Anal. Biochem. 318: 52-59 (2003) https://doi.org/10.1016/S0003-2697(03)00135-0
  23. Parker MA, Anderson JK, Corliss DA, Abraria VE, Sidman RL, Park KI, Teng YD, Cotanche DA, Snyder EY. Expression profile of an operationally-defined neural stem cell clone. Exp. Neurol. 194:320-332 (2005) https://doi.org/10.1016/j.expneurol.2005.04.018
  24. Niculescu AB, Chen X, Smeets M, Hengst L, Prives C, Reed SI. Effects of p21(Cip1/Waf1) at both the G1/S and the G2/M cell cycle transitions: pRb is a critical determinant in blocking DNA replication and in preventing endoreduplication. Mol. Cell. Biol. 18:629-643 (1998) https://doi.org/10.1128/MCB.18.1.629
  25. May P, May E. Twenty years of p53 research: Structural and functional aspects of the p53 protein. Oncogene 18: 7621-7636 (1999) https://doi.org/10.1038/sj.onc.1203285
  26. Bai F, Matsui T, Ohtani-Fujita N, Matsukawa Y, Ding Y, Sakai T. Promoter activation and following induction of the p21/WAF1 gene by flavone is involved in G1 phase arrest in A549 lung adenocarcinoma cells. FEBS Lett. 437: 61-64 (1998) https://doi.org/10.1016/S0014-5793(98)01198-3
  27. Oakea SA, Lin SS, Bassik MC. The control of endoplasmic reticulum-initiated apoptosis by the BCL-2 family of proteins. Curr. Mol. Med. 6: 99-109 (2006) https://doi.org/10.2174/156652406775574587
  28. van Delft M, Huang DC. How the Bcl-2 family of proteins interact to regulate apoptosis. Cell Res. 16: 203-213 (2006) https://doi.org/10.1038/sj.cr.7310028