Analysis of Fluoroquinolone Antibiotics in Foods

식품 중 플루오로퀴놀론계 항생제의 분석

Residual fluoroquinolone levels in animal foods retailed in Korea were monitored according to the method outlined in Korea Food Code using HPLC-FLD and HPLC-ESI-MS/MS for confirmation. The optimum ion transitions were $360{\rightarrow}316$, 342 m/z for enrofloxacin, $332{\rightarrow}314$, 288 m/z for ciprofloxacin, $320{\rightarrow}301$, 230 m/z for norfloxacin, $334{\rightarrow}315$, 290 m/z for pefloxacin, $362{\rightarrow}318$, 261, 334 m/z for ofloxacin, and $262{\rightarrow}201$, 126 m/z for flumequin. Enrofloxacin, ciprofloxacin and norfloxacin residues were found in 12 out of 388 samples. These antibiotics were only found in chicken samples, while no residues were found in beef, pork, milk and egg samples. Using this monitoring method, detection rates of 3.1, 1.3, and 0.3% were obtained for enrofloxacin, ciprofloxacin and norfloxacin, respectively. The levels of enrofloxacin, ciprofloxacin and norfloxacin detected in food samples ranged from 0.01 to 0.73 mg/kg in 12 samples, 0.01-0.03 mg/kg in 5 samples, and 0.12 mg/kg in only a sample, respectively.

총 388건의 시료를 분석한 결과, 닭 143건 중 총 12건의 플루오르퀴놀론계 항생제가 검출되었으며 다른 식품군에서는 모두 불검출 되었다. 항생제 별로 보면 엔로플록사신이 12건으로 가장 많았고 시프로플록사신이 5건, 노르플록사신이 1건 순이었다. 검출농도는 엔로플록사신이 0.01-0.73 mg/kg, 시프로플록사신이 0.01-0.03 mg/kg 및 노르플록사신이 0.12 mg/kg이었다.