Analysis and Evaluation of Degrees of Contribution of Aroma Components in Hongro Apples

홍로사과의 향에 영향을 주는 향 성분 분석과 기여도 평가

  • Koh, Jin-Tae (Department of Environmental Science, Kangwon National University) ;
  • Yu, Young-Jae (Department of Environmental Science, Kangwon National University) ;
  • Kim, Man-Goo (Department of Environmental Science, Kangwon National University)
  • 고진태 (강원대학교 자연과학대학 환경과학과) ;
  • 유영재 (강원대학교 자연과학대학 환경과학과) ;
  • 김만구 (강원대학교 자연과학대학 환경과학과)
  • Published : 2009.12.31

Abstract

In this study, "Hongro" apples for test samples were selected from a market for aroma analysis. Analysis was done after 1 hr, in a forming headspace while maintaining a temperature of $25^{\circ}C$. First, the complex aroma of the apples was assessed by a Direct Sensory Method. Secondly, the complex aroma was analyzed under individual aroma conditions separated by GC/FID/Olfactometry. Finally, aroma component analysis by GC/MS was performed. Degrees of contribution of aroma components were evaluated by an aroma value calculation considering aroma duration time, frequency, and intensity. The contribution rate (%) of the aroma induction component influencing apple aroma was determined by aroma component analysis and aroma contribution degree. As a result, it was found that the top four components were as follows, by contribution rate (%): acetic acid (23%), 1-hexanol (16%), butyl ethanoate (13%), 4-methoxy-2-methylbutane (9%). These four components constitute the complex aroma tested by the direct sensory method, and was largely recognized by the apple aroma test panel. Consequently, it was found that these components are the key factors in apple aroma. If the mechanism of formation of these components can be found, it could have a significant influence on consumers' acceptance of new varieties of apples.

이 연구에서는 사과 홍로의 향 성분들을 분석하고 기여도를 평가하였다. 사과는 시중에서 유통 중인 홍로를 선택하여 헤드스페이스를 형성시킨 후 $25^{\circ}C$에서 1시간 보관하여 분석을 실시하였다. 향기의 분석은 먼저 관능검사법으로 사과의 복합향기를 측정하고, GC/FID/Olfactometry를 사용하여 개별향기로 분리하였을 때 복합향기의 향기가 검출되는지 확인한 뒤 GC/MS를 이용하여 향기성분을 분석하였다. 향기성분의 기여도는 향기를 수치화하기 위하여 향기지속 시간과 향기강도를 고려하여 향기 값을 계산하여 평가하였다. 향기성분의 분석과 기여도 평가를 통하여 사과의 향에 영향을 주는 향기유발성분의 기여율(%)을 구할 수 있었다. 그 결과 기여율(%)이 가장 높게 나타난 물질은 acetic acid(23%), 1-hexanol(16%), butyl ethanoate(13%), 2-methyl-butyl butanoate (9%) 등 4개의 물질로 나타났다. 이 물질은 관능검사법으로 평가한 복합향기와도 일치하며 향기평가요원들이 가장 많이 인지한 향기이기도 하다. 이것으로 이 물질이 사과의 향을 결정짓는 중요한 인자로 작용한다는 것을 알 수 있었다. 이 물질의 생성 메커니즘을 파악하게 된다면 새로운 사과의 품종 개발에 있어 주요한 영향을 미칠 것이며 소비자의 구매만족도를 향상시킬 수 있을 것이다.

Keywords

References

  1. Kim JK, Seo HH. Causes of tree vigor weakening and occurrence of deformed fruit in 'Hongro' apple trees. Korean J. Hort. Sci. Technol. 25: 408-412 (2007)
  2. Wong NP, Parks OW. Simple technique for extracting flavor compounds from fatty foods. J. Dairy Sci. 51: 1768-1769 (1968) https://doi.org/10.3168/jds.S0022-0302(68)87274-1
  3. Singleton VL. An extraction technique for recovery of flavors, pigments, and other constituents from wines and other aqueous solutions. Am. J. Enol. Viticult. 12: 1-8 (1961)
  4. Xu Y, Fan W, Qian MC. Characterization of aroma compounds in apple cider using solvent-assisted flavor evaporation and headspace solid-phase microextraction. J. Agr. Food Chem. 55: 3051-3057 (2007) https://doi.org/10.1021/jf0631732
  5. Madruga MS. Elmore JS, Dodson AT, Mottram DS. Volatile flavour profile of goat meat extracted by three widely used techniques. Food Chem. 115: 1081-1087 (2009) https://doi.org/10.1016/j.foodchem.2008.12.065
  6. Forss D, Holloway G. Recovery of volatile compounds from butter oil. J. Am. Oil Chem. Soc. 44: 572-575 (1967) https://doi.org/10.1007/BF02901252
  7. Maeztu L, Sanz C, Andueza S, Paz De Pena M, Bello J, Cid C. Characterization of espresso coffee aroma by static headspace GC-MS and sensory flavor profile. J. Agr. Food Chem. 49: 5437-5444 (2001) https://doi.org/10.1021/jf0107959
  8. Elmore JS, Erbahadir MA, Mottram DS. Comparison of dynamic headspace concentration on tenax with solid phase microextraction for the analysis of aroma volatiles. J. Agr. Food Chem. 45: 2638-2641 (1997) https://doi.org/10.1021/jf960835m
  9. L$\acute{o}$pez ML, Lavilla MT, Riba M, Vendrell M. Comparison of volatile compounds in two seasons in apples: Golden Delicious and Granny Smith. J. Food Quality 21: 155-166 (1998) https://doi.org/10.1111/j.1745-4557.1998.tb00512.x
  10. Dixon J, Hewett EW. Factors affecting apple aroma/flavour volatile concentration: A review. New Zeal. J. Crop Hort. Sci. 28: 155-173 (2000) https://doi.org/10.1080/01140671.2000.9514136
  11. Mehinagic E, Royer G, Symoneaux R, Jourjon F, Prost C. Characterization of odor-active volatiles in apples: influence of cultivars and maturity stage. J. Agr. Food Chem. 54: 2678-2687 (2006) https://doi.org/10.1021/jf052288n
  12. L$\acute{o}$pez ML, Lavilla MT, Recasens I, Graell J, Vendrell, M. Changes in aroma quality of 'Golden Delicious' apples after storage at different oxygen and carbon dioxide concentrations. J. Sci. Food Agr. 80: 311-324 (2000) https://doi.org/10.1002/1097-0010(200002)80:3<311::AID-JSFA519>3.0.CO;2-F
  13. Pudil F, Viden I, Veli$\check{s}$k J, Davidek J. The volatile components of an industrial apple aroma concentrate. Z. Lebensm. Unters. For. 177: 181-185 (1983) https://doi.org/10.1007/BF01146792
  14. Girard B, Lau OL. Effect of maturity and storage on quality and volatile production of 'Jonagold' apples. Can. I. Food Sci. Tech. J. 28: 465-471 (1995) https://doi.org/10.1016/0963-9969(96)81393-7
  15. Lurie S, Pre-Aymard C, Ravid U, Larkov O, Fallik, E. Effect of 1-methylcyclopropene on volatile emission and aroma in cv. Anna apples. J. Agr. Food Chem. 50: 4251-4256 (2002) https://doi.org/10.1021/jf0200873
  16. Hwang YS. After the apple harvest management and storage technologies for export. Vol. 195, pp. 48-68. IN: Korea Agricultural Trade Information. Korea Agro-Fisheries Trade Corporation, Seoul, Korea (2005)
  17. Kim MG, Jung YR, Park JJ, Seo YM, Yoon IG. Evaluation metohd of odor-active compounds combine sensory method with instrumental analysis. Korean J. Odor Res. Eng. 1: 31-38 (2002)
  18. Kim KH. A study on identification of odor active compounds emitted from an automobile air-conditioner evaporator. MS thesis, Kangwon National University, Gangwon, Korea (2007)
  19. National Institute of Environmental Research. Standard method of odor compounds. Available from: http://www.nier.go.kr Accessed Nov. 25, 2009
  20. Kim SH. A study on identification and quantitation of individual odors forming a complex odor. MS thesis, Kangwon National University, Gangwon, Korea (2008)