Antifungal Activity of Impatiens balsamina against Ginseng Pathogen Alternaria panax

봉선화(Impatiens balsamina)의 인삼점무늬병균에 대한 항균활성

  • 안태진 (충북 음성군 농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 신유수 (충북 음성군 농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이승은 (충북 음성군 농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 안영섭 (충북 음성군 농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영국 (충북 음성군 농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박충범 (충북 음성군 농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 유승헌 (충남대학교 농업생명과학대학)
  • Published : 2009.12.30

Abstract

This study was conducted to find out environment-friendly disease control method to Alternaria blight caused by Alternaria panax of ginseng. For this study, 150 methanol extracts from medicinal plants were evaluated and the extract of Impatiens balsamina showed most strong antifungal activity against A. panax. The methanol extract of I. balsamina showed also stable antifungal activity against other ginseng pathogens such as Botrytis cinerea and Fusarium sp., when treated by heat or pH. In vivo, Alternaria blight incidence rate was low of 13% with the treatment of I. balsamina methanol extract compared to 35% of the non-treatment. The antifungal compound of I. balsamina was purified and identified by using a silica gel column chromatography, TLC and ESI-LC/MS/MS analysis. The compound which showed strong antifungal activity was identified as 2-methoxy-1,4-naphtoquinone ($C_{11}H_8O_3$).

Keywords

References

  1. Yu SH, Yun HK, Kim WG, Lee YH and Lee EJ. (1992). Alternaria leaf spot of Aralia cordata caused by Alternaria panax. Korean Journal of Plant Pathology. 8:81-84
  2. Yu SH. (2001). Korean species of Alternaria and Stemphylium. Rural Development Administration. Suwon, Korea. p. 85, 100
  3. Lee EJ, Lee YH, Cho WD, Kim WK and Jin KS. (1991). Compendium of medicinal plant diseases with plates. Rural Development Administration. Suwon, Korea. p. 90-91
  4. De Waard MA, Georgopoulos SG, Hollomon DW, Ishii H, Leroux P, Ragsdale NN and Schwinn FJ. (1993). Chemical control for plant disease : problems and prospects. Annual Review of Phytopathology. 31:403-421 https://doi.org/10.1146/annurev.py.31.090193.002155
  5. Paik SB and Oh YS. (1990). Screening for antifungal medicinal plants controlling the soil borne pathogen, Pythium ultimum. Korean Journal of Mycology. 18:102-108
  6. Paik SB, Kyung SH, Doh ES, Oh YS and Park BK. (1994). Screening and identification of fungicidal compounds derived from medicinal plants against cucumber powdery mildew. Korean Journal of Environmental Agriculture. 13:301-310
  7. Yu SY, Kim JC, Kim YS, Kin HT, Kim SK, Choi KJ, Kim JS, Lee SW, Heu JH and Cho KH. (2001). Antifungal activities against plant pathogens of coumarins isolated from Angelica gigas and Angelica dahurica. The Korean Journal of Pesticide Science. 5:26-35
  8. Choi GJ, Kim JC, Jang KS, Lee SW, Kim JS and Cho KY. (2002). Antifungal activities of several plant extracts against wheat leaf rust. The Korean Journal of Pesticide Science. 6:87-95
  9. John EL, Thomas JS and Murray WF. (1948). Isolation and antifungal action of naturally occurring 2-methoxy-1,4-naphthoquinone. The Journal of Biological Chemistry. 174:335-342
  10. Kang SC and Moon YH. (1992). Isolation and antimicrobial activity of naphthoquinone from Impatiens balsamina. Korean Journal of Pharmacognosy. 23:240-247
  11. Lee DG, Shin SY, Kim DH, Seo MY, Kang JH, Lee YH, Kim KL and Hahm KS. (1999). Antifungal mechanism of a cysteine-rich antimicrobial peptide, b-AMP1, from Impatiens balsamina against Candida albicans. Biotechnology Letters. 21:1047-1050 https://doi.org/10.1023/A:1005636610512
  12. Karin T, Isabelle EJA, Lolke S, Aart VA, Wim MM, Rob M, Truus P, Willem FB and Bruno PA. (2005). Antifungal activity of synthetic peptides derived from Impatiens balsamina antimicrobial peptides b-AMP1 and b-AMP4. Journal of Peptides. 26:1113-1119 https://doi.org/10.1016/j.peptides.2005.01.008