Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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High Pressure Extraction Process of Low Quality Fresh Ginseng for Enhancing Anticancer Activities

파삼의 항암활성 증진이 가능한 고압 추출 공정

  • Ha, Ji-Hye (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Young (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Jeong, Seung-Seop (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Jeong, Myoung-Hoon (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Jeong, Heon-Sang (College of Agriculture, Life & Environment Sience, Chungbuk National University) ;
  • Jeong, Jae-Hyun (Department of Food and Biotechnology, Chungju National University) ;
  • Yu, Kwang-Wan (Department of Food and Biotechnology, Chungju National University) ;
  • Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National University)
  • 하지혜 (강원대학교 BT특성화학부대학) ;
  • 김영 (강원대학교 BT특성화학부대학) ;
  • 김승섭 (강원대학교 BT특성화학부대학) ;
  • 정명훈 (강원대학교 BT특성화학부대학) ;
  • 정헌상 (충북대학교 식품공학과) ;
  • 정재헌 (충주대학교 식품생명공학과) ;
  • 유광원 (충주대학교 식품생명공학과) ;
  • 이현용 (강원대학교 BT특성화학부대학)
  • Published : 2009.12.30
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Abstract

The low quality fresh ginseng was extracted by water at $80^{\circ}C$ and 240 bar for 20 min (HPE, High pressure extraction process). The cytotoxicity on human normal kidney cell (HEK293) and human normal lung cell (HEL299) of the extracts from HPE showed 28.43% and 21.78% lower than that from conventional water extraction at $100^{\circ}C$ in adding the maximum concentration of $1.0\;mg/m{\ell}$. The human breast carcinoma cell and lung adenocarcinoma cell growth were inhibited up to about 86%, in adding $1.0\;mg/m{\ell}$ of extracts from HPE. This values were 9-12% higher than those from conventional water extraction. On in vivo experiment using ICR mice, the variation of body weight of mice group treated fresh ginseng extracts from HPE of 100 mg/kg/day concentration was very lower than control and other group. The extracts from HPE was showed longer survival times as 35.65% than that of the control group, and showed the highest tumor inhibition activities compared with other group, which were 70.64% on Sarcoma-180 solid tumor cells. On the high performance liquid chromatogram (HPLC), amount of ginsenoside-$Rg_2$, $Rg_3$, $Rh_1$ and $Rh_2$ on fresh ginseng were increased up to 43-183% by HPE, compared with conventional water extracts. These data indicate that HPE definitely plays an important role in effectively extracting ginsenoside, which could result in improving anticancer activities. It can be concluded that low quality fresh ginseng associated with this process has more biologically compound and better anticancer activities than that from normal extraction process.

Keywords

References

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