Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Proapoptotic and antitumor effect of Hangbaek-Tang(HBT) in a tumor transplanted mouse model

마우스 모델에서 항백탕 투여에 의한 종양 증식의 억제 및 Apoptosis의 유도

  • Yun, Young-Gab (Department of Oriental Medical Prescription, Wonkwang University) ;
  • Kim, Jun-Hee (Department of Biological Science, Graduate School, Jeonju University) ;
  • Song, Eun-Jung (Department of Biological Science, Graduate School, Jeonju University) ;
  • Hwang, Jin-Ki (Department of Biological Science, Graduate School, Jeonju University) ;
  • Nam, Sang-Yun (Department of Biological Science, Graduate School, Jeonju University)
  • 윤용갑 (원광대학교 한의과대학 방제학교실) ;
  • 김준희 (전주대학교 대학원 생명과학과) ;
  • 송은정 (전주대학교 대학원 생명과학과) ;
  • 황진기 (전주대학교 대학원 생명과학과) ;
  • 남상윤 (전주대학교 대학원 생명과학과)
  • Published : 2009.12.30
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Abstract

Objective : In vitro proapoptotic effect of Hangbaek-Tang (HBT) has been documented by one of us. In the present study, we aimed to demonstrate in vivo effect of HBT on tumor growth. Methods : In vitro selective cytotoxicity of HBT was examined by enumeration of viable cell numbers using BC3A mouse leukemic cells and normal spleen cells. In vivo effect of HBT (25 and 50 mg/mouse) on tumor growth was assayed using BC3A cells innoculated subcutaneously in the flank. Annexin-V apoptosis assay and PI staining was performed to determine the effective serum factor in HBT-treated mice. Leukocyte recruitment into peritoneum were analyzed by microscopy with a stained cytosmear of peritoneal lavage fluid. Results : HBT exhibited in vitro selective cytotoxicity to leukemic cells and did not show any toxicity on immune organs. In vivo i.p. administration of HBT induced significant reduction in tumor growth but not complete regression. Sera obtained from HBT-treated mice strongly inhibited BC3A cell growth in vitro and were revealed to markedly enhance apoptosis and accompanying cell death, when compared to those from PBS-treated mice. Abundant extravasation of leukocytes, especially neutrophils, into peritoneum was observed in HBT-treated mice. Conclusions : HBT causes leukemic, BC3A cell death in vivo via apoptosis as well as in vitro, for which functional involvement of leukocytes is suggested.

Keywords

References

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