Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지

Herbal Acupuncture of Nidus Vespae Suppresses c-Fos Expression by TNBS Induced Colitis in Rats

TNBS로 유도된 흰쥐의 대장염(大腸炎)에 대한 노봉방(露蜂房) 약침(藥鍼)의 효과

  • Song, Jeong-Bang (Cho-min's Korean Medical Clinics) ;
  • Kim, Jae-Hyo (Dept. of Meridian & Acupoint, College of Korean Oriental Medicine, Wonkwang University) ;
  • Kim, Yu-Lee (Dept. of Meridian & Acupoint, College of Korean Oriental Medicine, Wonkwang University) ;
  • Park, Yu-Ree (Dept. of Meridian & Acupoint, College of Korean Oriental Medicine, Wonkwang University) ;
  • Ahn, Seong-Hun (Dept. of Meridian & Acupoint, College of Korean Oriental Medicine, Wonkwang University) ;
  • Sohn, In-Chul (Dept. of Meridian & Acupoint, College of Korean Oriental Medicine, Wonkwang University)
  • 송정방 (초민한의원) ;
  • 김재효 (원광대학교 한의과대학 경락경혈학교실) ;
  • 김유리 (원광대학교 한의과대학 경락경혈학교실) ;
  • 박유리 (원광대학교 한의과대학 경락경혈학교실) ;
  • 안성훈 (원광대학교 한의과대학 경락경혈학교실) ;
  • 손인철 (원광대학교 한의과대학 경락경혈학교실)
  • Published : 2009.12.27
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Abstract

Objectives : Transient inflammation has been demonstrated to alter visceral sensory function in animal models and acute mucosal inflammation may precede the manifestation of visceral hyperalgesia. Thus in this study we compared effects of herbal acupuncture of Nidus Vespae (NV) applied to the different acupoints in the acute colitis induced by trinitrobenzenesulphonic acid (TNBS) intracolonic injection in rats. Methods : In Male Sprague-Dawley rats, weighing 250 ~ 400 g, TNBS (5 mg/kg) was infused intrarectally through a silicon rubber catheter into the anus under isoflurane anaesthesia. Under general anesthesia, acupoints of LI4 (Hapkok), SI25 (Cheonchu), ST36 (Joksamni), BL25 (Daejangsu) were intramuscularly injected by NV. Expressions of cFos protein in the periaqueductal gray (PAG), locus coeruleus (LC), nucleus of solitary tract (Sol), and the 6th lumbar spinal cord (L6 s.c.) were observed at 24 hrs after TNBS induced colitis by immunohistochemistry. Results : The expression of c-Fos protein in L6 s.c., Sol, LC and PAG increased 24 hrs after TNBS injection into colorectum as compared to normal group. NV herbal acupuncture also inhibited the expression of c-Fos protein in Sol but not L6 s.c., LC, and PAG. NV to ST36 inhibited significantly the c-Fos expression in Sol and PAG. NV to ST25 inhibited the c-Fos protein expression all over the observation area. NV to BL25 showed the inhibitory effects in the areas except LC. Whether or not a role of endogenous opioids, intrathecal injection of naltrexone (30 ug / 30 ul) was applied before the 2nd herbal acupuncture treatment 24 hrs after TNBS-induced colitis in rat. Naltrexone reversed the inhibition of c-Fos protein expression in the spinal cord and brainstem under different conditions such as type of herbal acupuncture compound and choice of acupoint. Conclusions : In summary, these data show that herbal acupuncture of NV inhibits signal pathways such as spinal cord and brain stem ascending hypersensitivity of colorectum after TNBS induced colitis. This effect may be mediated by acupoints through the endogenous opioid system involving the pain modulation.

Keywords

References

  1. 강태희, 원진희. 궤양성 대장염 치료의 한의학적 접근방식에 관한 연구. 대한성인병 학회지. 1999 ; 5(1) : 137-62.
  2. 김우호. 흰쥐에서 TNBS로 유발된 염증성대장염의 병태생리에 관한연구. 대한내과학회지. 1994 : 47(1) : 20-35.
  3. Kirsner JB, Shorter RG. Recent developments in 'non specific' inflammatory bowel disease. N Engl Med. 1982 ; 306 : 775-80. https://doi.org/10.1056/NEJM198204013061304
  4. Birder LA, Kiss S, de Groat WC, Lecci A, Maggi CA. Effect of nepadutant, a neurokinin 2 tachykinin receptor antagonist, on immediate-early gene expression after trinitrobenzenesulfonic acid-induced colitis in the rat. J Pharmacol Exp Ther. 2003 ; 304(1) : 272-6. https://doi.org/10.1124/jpet.102.042077
  5. Yamada T, Marshall S, Specian R, Grisham MB. A comparative analysis of two models of colitis in rat. Gastroenterology. 1992 ; 102 : 1524-34.
  6. Tibbles LA, Woodgett JR. The stress activated protein kinase pathways. Cell Mol Life Sci. 1999 ; 55 : 1230. https://doi.org/10.1007/s000180050369
  7. Henninger DD, Panns J, Eppihimer M, Russell J, Gerritsen M, Anderson DC and Granger DN. Cytockine-induced VCAM-1 and ICAM-1 expression in different organs of the mouse. J Immunol. 1997 ; 158 : 1825-32.
  8. 대한소화기학회. 염증성질환. 서울 : 군자 출판사. 1999 : 1-6.
  9. 송문영, 박상연, 김재효, 안성훈, 김경식, 손인철. TNBS로 유도된 대장염에서 곡지의 구진약침 효과. 경락경혈학회지.2008 ; 25(3) : 147-66.
  10. 최윤영, 안성훈, 권오상, 서상록, 손인철, 김재효. 흰쥐의 TNBS 유발 대장염에서 전침의 대장 흥분성에 미치는 효과. 경락 경혈학회지. 2009 ; 26(2) : 91-108.
  11. 文漢英. 中藥內服加保留灌腸治療慢性結 腸炎64例. 江西中醫藥. 1998 ; 29(5) : 28.
  12. 변기원, 임용수, 구성태, 김재효, 김경식, 손인철. 합곡에 대한 애엽 약침이 흰쥐 대장염 완화에 미치는 효과. 대한경락경혈학회지. 2007 ; 24(1) : 79-94.
  13. 박용기. 露峰房의 抗酸化作用에 關한 硏究. 대한본초학회지. 2002 ; 17(2) : 203-12.
  14. 장재식. 노봉방(露蜂房)이 t-butylhydroxyperoxide에 의한 Peroxynitrite 생성과 염증성 단백질 발현에 미치는 영향. 대한동의생리학회. 2007 ; 21(6) : 1499-505.
  15. 정지천, 신원용. 노봉방(露峰房)의 Peroxynitrite 제거 효과. 대한한의학회지. 2006 ; 27(2) : 171-81.
  16. 대한약침학회편저. 약침요법시술지침서. 서울 : 대한약침학회. 2000 : 13, 128-33.
  17. 전국한의과대학.한의학전문대학원 경락경혈학 교재편찬위원회. 대학경락경혈학각론. 원주: 의방출판사. 2009 ; 73, 228, 259, 600.
  18. 洪彰義. 臨床小兒科學槪論. 서울 : 大韓 敎科書株式會社. 1975 : 351-2.
  19. 周命新. 醫門寶鑑. 서울 : 杏林書院. 1971 : 259.
  20. 張介賓. 張氏景岳全書. 서울 : 翰成社. 1983 : 439-47.
  21. 董黎明. 實用中醫內科學. 上海: 上海科 學技術出版社. 1988 : 238-43.
  22. 本間祥白著. 難經之硏究. 정언출판사인행. 337-40.
  23. Rachmilewitz D, Simon PL, Schwartz LW, Griswold DE, Fondacaro JD, Wasserman MA. Inflammatory mediators of exprimental colitis in rats. Gastroenterology. 1989 ; 97 : 326.
  24. 김나영, 김재준, 송인성, 김정룡, 김우호. 흰쥐에서 TNBS로 유발된 염증성대장염의 병태생리에 관한 연구. 대한내과학회지. 1994 ; 47 : 20-35.
  25. Wallace JL, MacNaughton WK, Morris GP, Beck PL. Inhibition of leukotriene synthesis markedly accelerates healing in a rat model of inflammatory bowel disease. Gastroentrology. 1989 ; 96 : 29.
  26. Floman Y, Zor U; Mechanism of steroid action in inflammation: Inhibition of prostaglandin synthesis and release. Prostaglandins. 1976 ; 12 : 403. https://doi.org/10.1016/0090-6980(76)90021-6
  27. 김정제. 최신침구학. 서울 : 성보사. 1995 : 211-3.
  28. 홍원식. 정교 황제내경영추. 서울 : 동양의학연구원출판부. 1981 : 21, 39, 123, 176.
  29. 林鐘國. 鍼灸治療學. 서울 : 集文堂. 1983 : 226-30, 274, 298, 352, 364, 368, 461, 595.
  30. 孫思邈. 鍼灸醫學典籍大系. 備急千金要 方. 大阪市: 出版科學統合硏究所. 1979; 卷22 : 88-90.
  31. 張介賓. 類經圖翼. 北京: 文光圖書有限 公司印行. 225.
  32. 楊繼洲. 鍼灸大成. 서울 : 杏林出版社. 1977 : 220-7.
  33. 전국한의과 대학 본초학교수. 本草學. 서울 : 영림사. 1991 ; 647-8.
  34. 江蘇新醫學院. 中藥大辭典. 대구 : 대성출판사. 1984 : 2406.
  35. Monti,M, Berti, E., Carminati, G.,and Cusini, M. Occupational and cosmetic dermatitis from propolis. Contact Dermatitis. 1983 ; 9 : 163-4.
  36. 權採. 兪孝通. 盧重禮. 朴允德등. 鄕藥集 成方. 평양 : 과학백과사전출판사. 1993 ; 5권 : 262.
  37. 張金鼎, 鄭治文. 北京: 中醫古籍出版社. 2002 : 197.
  38. 許浚. 東醫寶鑑. 서울 : 대성문화사. 1992 : 197.
  39. Scheller S, Witczok T, Imielski S, Krol W, Gabrys J, Shani J. Free radical scavenging by ethanol dxtract of propolis. Int J Radiat Biol. 1990 ; 57(3) : 461-5. https://doi.org/10.1080/09553009014552601