대한본초학회지 (The Korea Journal of Herbology)

  • 제24권4호
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  • Pages.137-142
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  • 2009
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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두릅 순에서 분리된 화합물의 항산화 활성

Antioxidant Activity of Isolated Compounds from the Shoot of Aralia elata Seem

  • 이기호 (대구한의대학교 한방식품약리학과) ;
  • 정지욱 (대구한의대학교 한약재약리학과) ;
  • 안은미 (대구한의대학교 한방식품약리학과)
  • Lee, Gi-Ho (Department of Herbal Foodceutical Science, Daegu Haany University) ;
  • Jung, Ji-Wook (Department of Herbal Medicinal Pharmacology, Daegu Haany University) ;
  • Ahn, Eun-Mi (Department of Herbal Foodceutical Science, Daegu Haany University)
  • 발행 : 2009.12.30
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초록

Objectives : This study was performed to investigate the antioxidant activities of isolated compounds from the shoot of Aralia elata. Methods : The methanol extract from the shoot of Aralia elata was fractionated into ethyl acetate, n-BuOH and $H_2O$ layers through solvent fractionation. Repeated silica gel, ODS column chromatography of n-BuOH layer afforded four flavonol glycosides. Their antioxidant activity was determined by measuring free radical scavenging activity by DPPH, ABTS and superoxide dismutase (SOD) like activity assay. Results : They were identified as quercetin 3,7-di-O-$\alpha$-rhamnopyranoside (1), quercetin 3-O-$\beta$-D-galactoside-7-O-$\alpha$-L-rhamnoside (2), kaempferol 3-O-$\beta$-glucosyl($1{\rightarrow}2$)-$\alpha$-rhamnoside-7-O-$\alpha$-rhamnoside (3) and quercetin 3-O-$\beta$-glucosyl($1{\rightarrow}2$)-$\alpha$-rhamnoside-7-O-$\beta$-rhamnoside (4) on the basis of spectroscopic data. The result showed that 1 is the most active compound in the DPPH and ABTS radical scavenging test. Conclusions : Isolated Compounds from the shoot of Aralia elata showed anti-oxidative effect.

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