Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Effect of Surfactin on Growth Performance of Weaning Piglets in Combination with Bacillus subtilis BC1212

바실러스 섭틸리스 BC1212와 설팩틴의 병용투여가 이유돈의 성장에 미치는 영향

  • Kim, Myoung-Seok (Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Lim, Jong-Hwan (B&CBiopharm) ;
  • Park, Byung-Kwon (JEONJINBIO) ;
  • Hwang, Yun-Hwan (Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Song, In-Bae (Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Park, Seung-Chun (College of Veterinary Medicine, Kyungpook National University) ;
  • Yun, Hyo-In (Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University)
  • 김명석 (충남대학교 수의과대학 동물의과학연구소) ;
  • 임종환 (비엔씨바이오팜) ;
  • 박병권 (전진바이오팜) ;
  • 황윤환 (충남대학교 수의과대학 동물의과학연구소) ;
  • 송인배 (충남대학교 수의과대학 동물의과학연구소) ;
  • 박승춘 (경북대학교 수의과대학) ;
  • 윤효인 (충남대학교 수의과대학 동물의과학연구소)
  • Published : 2009.04.30
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Abstract

The aim of this study is to investigate the effects of surfactin in combination with Bacillus subtilis BC1212 isolated from Korean soybean paste, on feed utilization and growth performance during 4 weeks in weaning piglets. Eighteen weaning piglets(Landrace$\times$Yorkshire$\times$Duroc; weighing $7.68{\pm}0.97\;kg$) were divided into control(n=9) and experimental groups(n=9). The treatments included a control group consisting of the basal diet with no additives(control) and an experimental group consisting of the basal diet supplemented with 1 g of surfactin C and $1.0{\times}10^9CFU$ of Bacillus subtilis BC1212/kg feed. Piglets fed Bacillus subtilis BC1212 increased in average daily weight gain and feed efficiency. In comparison with the control group, the fecal Bacillus subtilis were significantly increased and the fecal coliform bacteria were markedly reduced in the experimental group. In addition, Bacillus subtilis BC1212 had excellent acid and bile tolerance. The treatment of surfactin($50{\mu}g\;ml^{-1}$) in lipopolysaccharide(LPS)-stimulated swine peripheral blood mononuclear cells(PBMCs) for 6 h showed a significant inhibitory effect on INF-$\gamma$, TNF-$\alpha$ and NO secretion(p<0.05) in comparison with LPS treatment alone but not on IL-10 secretion, with levels of secreted IL-10 similar to those secreted by PBMCs stimulated with LPS alone. Supplementation with surfactin in combination with Bacillus subtilis BC1212 in diets improved the ecosystem of gastrointestinal tract by increasing probiotic population and enhanced the systemic immune response in weaned piglets.

한국된장에서 분리한 바실러스섭틸리스 BC1212와 설팩틴을 함유한 사료첨가제가 이유돈의 성장에 미치는 영향을 연구하였다. 총 18마리의 이유돈(랜드레이스$\times$요크셔$\times$듀록; 체중, $7.68{\pm}0.97\;kg$)을 9마리씩 두 그룹으로 배치하여 실험을 실시하였다. 실험군은 사료 kg 당 설팩틴 C 1 g과 $1.0{\times}10^9CFU$ 바실러스섭틸리스 BC1212를 급여하였고, 대조군은 일반사료를 4주간 급여하였다. 그 결과 바실러스섭틸리스 BC1212와 설팩틴을 급여한 군에서는 일평균 증체량과 사료효율이 증가하였으며, 무처리 대조군과 비교해서, 분변 중 바실러스-섭틸리스의 생존률이 현저히 높았다. 또한 바실러스섭틸리스는 산과 담즙에 대한 높은 내성을 보였다. 세포 내 독소 투여 후 말초혈액단액세포에 설팩틴($50{\mu}g\;ml^{-1}$) 처리 후 약 6 시간까지 INF-$\gamma$, TNF-$\alpha$ 과 NO 분비량을 설팩틴 무처리군에 비해 현저히 감소시켰다(p<0.05). 그러나 IL-10의 분비량은 설팩틴 무처리군과 유사했다. 결론적으로 바실러스섭틸리스 BC1212와 설팩틴을 병용하여 이유돈에 급여할 경우, 이유돈의 위장관내 생균수 증가와 함께 면역반응을 증대시킬 수 있다.

Keywords

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