Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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BONE HEALING CAPACITY IN THE FRACTURE OF RABBIT MANDIBULAR BONE USING LOW-LEVEL LASER

가토 하악골 절단부의 저수준 레이저 처치가 골치유에 미치는 영향

  • Bae, Yong-Hyeon (Department of Oral and Maxillofacial surgery, College of Dentistry, Dankook University) ;
  • Han, Se-Jin (Department of Oral and Maxillofacial surgery, College of Dentistry, Dankook University) ;
  • Kim, Kyung-Wook (Department of Oral and Maxillofacial surgery, College of Dentistry, Dankook University)
  • 배용현 (단국대학교 치과대학 구강악안면외과학교실) ;
  • 한세진 (단국대학교 치과대학 구강악안면외과학교실) ;
  • 김경욱 (단국대학교 치과대학 구강악안면외과학교실)
  • Published : 2009.04.30
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Abstract

The concept of biostimulation of wounds by low-level laser therapy(LLLT) is attracting considerable attention. Although its effect on whole tissues has been studied quite extensively, the biological and cellular mechanisms underlying LLLT have not been clarified. In an experimental radius fracture in rabbits, Tang and Chai reported that LLLT enhanced the activity of red blood cells, macrophages, fibroblasts, chondrocytes, and osteoclasts within the fracture area. The purpose of the present study was to evaluate the effect of LLLT with a GaAlAs diode laser device on bone healing in rabbit mandibular fractures. We use 12 rabbits for this study. All rabbits were fractured mandible angle area using saw in anesthetic condition. In control group(n=6), none treatment was performed at fracture site. In experimental group(n=6), LLLT with a GaAlAs diode laser was radiated at fracture site daily for 7 days. All rabbits were sacrificed at 6 weeks later from performed fracture day. We studied the immunohistochemical staining of CD34 and Vimentin and the histochemical analysis for calcium and phosphorus content. The results were as follows. 1. In the histological and immunohistological staining, after 6week, fibroblasts, osteogenic cells and collgen fibers were observed more in experimental group than in control group. 2. In the histochemical analysis, the amount of calcium and phosphorus contents of the experimental group were more than the control group. From the results obtained, we suggest that the bone healing is stimulated by low-level laser irradiation in bone fractures.

Keywords

References

  1. Einstein A : Zur quantem theorie der strahlung. Phys Zeit 1917; 18:121
  2. Ossoff RH and Karlan Ms : Laser surgery in otolaryngology. In Ballenger J, 1985
  3. Cernavia I, Pugatschew A, de Boer N : Laser applications in Dentistry : a review of the literature. Australian Dental Journal 1994;39:28-32 https://doi.org/10.1111/j.1834-7819.1994.tb05543.x
  4. Walsh LJ : The Current status of low level laser therapy in dentistry. Part I; Soft tissue applications. Australian Dental Journal 1997;42: 247-254 https://doi.org/10.1111/j.1834-7819.1997.tb00129.x
  5. Mester E : Clinical results of wound healing stimulation studies with laser and experimental studies of the action mechanism. Laser optoelectronics1976;119-123
  6. Lievens P : Effects of laser irradiation on the lymph system and wound healing. Pharmakon 1986;66:65-71
  7. Pick RM : Laser in denstry. soft tissue procedure Dent Clin North Am1993;37:281-290
  8. Miller M : Laser in denstry. J Am Dent Assoc 1993;124:32-38
  9. Clokie C : The effects of the He-Ne laser on postsurgical discomfort. J Can Dent Assoc 1991;52:584
  10. Kert J : Low level laser therapy used pre-operatively. Laser News 1992;4:26-32
  11. Roynesdal AK and Bjorland T : The effect of soft laser applicationon post-operative pain and swelling. Int J Oral Maxillofac Surg 1993;22:242-251 https://doi.org/10.1016/S0901-5027(05)80646-0
  12. Bosata M, Jucci A, Olliaro P, Quacci D, Sacchi S. : In vitro fibroblastdermis fibroblast activation by laser irradiation at low energy. Dermatologic1984;168:157-62 https://doi.org/10.1159/000249691
  13. Tang XM and Chai BP : Effect of CO2 laser irradiation on experimental fracture healing; a transmission electron microscopic study. Lasers Surg Med. 1986;6: 346-352 https://doi.org/10.1002/lsm.1900060309
  14. Elchanan J, Semon R, Yoram W, Galina K, Shmuel D. : Effect of low-power laser irradiation on the mechanical properties of bone frecture healing in rats. Laser in Surgery and Medicine. 1998;22: 97-102 https://doi.org/10.1002/(SICI)1096-9101(1998)22:2<97::AID-LSM5>3.0.CO;2-R
  15. LJ Miserendino and RM Pick : Lasers in Dentistry. Quintessence Publishing Co, Inc 1995
  16. Mona K Marei, Shadia H Abdel-Meguid, Sherif A Mokhtar, et al : Effect of low-energy laser application in the treatment of dentureinduced mucosal lesions. J prosthet Dent 1997;77:256-261 https://doi.org/10.1016/S0022-3913(97)70182-3
  17. Yasunori Takeda : Irradiation effect of low energy laser on alveolar bone after tooth extraction. Int. J Oral Maxillofac Surg 1988;17:388-395 https://doi.org/10.1016/S0901-5027(88)80070-5
  18. Ki-Suk Kim : Effects of low level laser irradiation with 904 nm pulsed diode laser on the extraction wound. J of Korean Academy of Oral Med 1998;23:301-309
  19. Masami Akai, Mariko Usuba, Toru Maeshima : Lasers effect on bone and cartilage change induced by joint immobilization. Laser in Surg andMed 1997;21:480-8 https://doi.org/10.1002/(SICI)1096-9101(1997)21:5<480::AID-LSM10>3.0.CO;2-D
  20. Maawan K, Nesrin K, Hans R, Jan E : Enhancement of bone formation in rat calvarial bone defects using low-level laser therapy. Oral Surg Oral Med Oral Patho. 2004;97: 693-700 https://doi.org/10.1016/j.tripleo.2003.11.008
  21. Lam TS, Abergel RP, Meeker CA, Castel JC, Dwyer RM, Uitto J : Laser stimulation of collagen synthesis in human skin fibroblast culture. LaserLifr Sci. 1986;1:61-77
  22. Oron U, Taakobi T, Oron A, Hayam G, Gepstein L, Rubin O : Attenuation of infarct size in rat and dog after myocardial infarction by lowenergy laser irradiation. Laser Surg Med. 2001;28: 204-211 https://doi.org/10.1002/lsm.1039
  23. Morimoto Y, Arai T, Kikuchi M, Nakajima S, Nakamura H : Effect of low intensity Argon laser irradiation on mitrochodria respiration. Laserds Surg Med. 1994;15:191-200 https://doi.org/10.1002/lsm.1900150207
  24. Yu X, McGowan M, Ippolito K, Lanzafame RJ. : Photomodulation of oxative metabolism and electron chain enzymes in rat liver mitochodria. Photochem Photobiol. 1997;66:866-71 https://doi.org/10.1111/j.1751-1097.1997.tb03239.x
  25. Chen JW, Zhou YC. : Effect of low level carbon dioxide laser radiationon biochemical metabolism of rabbit mandibular bone callus. Laser Therapy1989;2:83-7
  26. Yoshihide Kobu. : Effects on infrared radiation on intraosseous blood flow and oxygen tension in the rat tibia. Kobe J Med Sci. 1999;45:27-39
  27. Robert F Labbe, Kristen J. Skogerboe : Laser photobioactivation mechanisms. Lasers in Surgery and Medicine 1990;10:201-210 https://doi.org/10.1002/lsm.1900100215
  28. Tuderman L, Myllyyla R, Kivirikko KI : Mechanism of the prolyhydroxylase reaction. Nutr Rev 1982;40:306 https://doi.org/10.1111/j.1753-4887.1982.tb05237.x
  29. Trelles M and Mayayo E : Bone fracture consolidates faster with lowpower laser. Lasers Surg Med 1986;7:36-45 https://doi.org/10.1002/lsm.1900070107