Insecticidal Activities and Synergism of Bistrifluron Against EPN-Resistant Strain of Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

EPN 저항성 배추좀나방에 대한 IGR계 약제 Bistrifluron의 살충활성과 세 가지 협력제에 의한 살충활성 증진 효과

  • Joo, You-Lee (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University) ;
  • Yang, Jeong-Oh (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University) ;
  • Yoon, Chang-Mann (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University) ;
  • Kim, Gil-Hah (Dept. of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University)
  • 주유리 (충북대학교 농업생명환경대학 식물의학과) ;
  • 양정오 (충북대학교 농업생명환경대학 식물의학과) ;
  • 윤창만 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Published : 2009.03.31

Abstract

EPN-resistant diamondback moth were tested the developmental characteristics, longevity, fecundity and synergistic effect with synergists when treated with the bistrifluron, an inhibitor of chitin synthesis. Inhibition rate of egg hatch was below at the recommended concentration (50 ppm) to the EPN-resistant strain than EPN-non selected strain, however, mortality within 24hr after hatching was showed as 50-60%. They did not show the difference between two strains. Mortality of larval instars were showed effective to two strains with no difference. Inhibition rate of emergence did not show any effect when treated with pupae. Moreover, they did not affect to the longevity and fecundity. When bistrifluron was treated at 50 ppm to the adult, however, longevity and fecundity was decreased with no difference between two strains. Bistrifluron to the EPN-resistant strain showed synergistic effect as high as 137.7 and 73.1 folds with synergist such as PBO (microsomal oxidase inhibitor) and polyoxin B (chitin synthetase inhibitor), respectively.

EPN-저항성 배추좀나방에 대한 chitin 생합성저해제인 bistrifluron의 발육단계별 살충활성, 성충수명 및 산란수에 미치는 영향을 조사하였다. 알에 대한 부화억제율은 추천농도(50 ppm)에서 낮았지만, 부화유충의 치사율이 50-60%를 나타내었고, 두 계통간에는 차이가 없었다 영기별 살충효과는 감수성 계통과 저항성 계통 모두에 효과가 있었다. 하지만 계통간에 차이는 크지 않았다. 번데기에 처리시 우화억제율은 거의 없었으며, 또 성충의 수명과 산란수에도 영향을 미치지 않았다. 성충에 처리했을 때 50 ppm농도에서 성충수명과 산란수는 감소하였지만 두계통간에 차이는 없었다. EPN저항성계통에 대한 bistrifluron과 협력제 piperonyl butoxide(microsomal oxidase inhibitor)와 polyoxin B(chitin synthetase inhibitor)는 각각 137.7배와 73.1배로 높은 협력작용을 나타내었다.

Keywords

References

  1. Abo-Elghar, G.E., A.E. EI-Sheikh, F.M. El-Sayed, H.M. EL-Maghraby and H.M. Ei-zun (2003) Perisitance and residual activity of an organophosphate, pirimiphos-methyl, and three IGRs, hexaflumuron, teflubenzuron and pyriproxyfen, against the cowpea weevil, Callosobruchus maculatus (Coleoptera:Bruchidae). Pest Manag. Sci. 60:95-102 https://doi.org/10.1002/ps.783
  2. Ahn. Y.J., G.H. Kim and K.Y. Cho (1992) Susceptibility of embryonic and postembryonic developmental stages of Riptortus clavatus (Hemiptera: Alydidae) to diflubenzuron. Korean J. Appl. Entomol. 31:480-485
  3. Arakawa, T. (2008) Synergistic effect of a fungicide containing polyoxin B with insect growth regulators(IGRs) in the killing of common cutworm, Spodoptera litura (Lepidoptera:Noctuidae). Appl. Entomol. Zool. 43:167-171 https://doi.org/10.1303/aez.2008.167
  4. Bernard, C.B. and B.J.R. Philogene (1993) Insecticide synergists:role, importance, and perspectives. J. Toxicol. Environ. Health 38:199-233 https://doi.org/10.1080/15287399309531712
  5. Butter, N.S., G. Singh and A.K. Dhawan (2003) Laboratory evaluation of the insect growth regulator lufenuron against Helicoverpa armigera on cotton. Phytoparasitica 31:200-203 https://doi.org/10.1007/BF02980790
  6. Cho, Y.S. and S.C. Lee (1994) Resistance development and cross-resistance of diamondback moth (Lepidoptera: Plutellidae) by single selection of several insecticides. Korean l. Appl. Entomol. 33:242-249
  7. Choi, Y.M., K.S. Ahn, I.C. Hwang, and G.H. Kim (2004) Property and mode of action of indoxacarb against diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Korean J. Appl. Entomol. 43:317-322
  8. Finney, D.J. (1971) Probit analysis. Cambridge Univ. Press, Cambridge. England. pp. 333
  9. Furlong, M.J. and D.J. Wright (1994) Examination of stability of resistance and cross-resistance patterns to acyl urea insect growth regulators in field populations of the diamondback moth, Plutella xylostella, from Malaysia. Pestic. Sci. 42:315-326 https://doi.org/10.1002/ps.2780420409
  10. Hajjar, N.P. and J.E. Casida (1979) Structure activity relationships of benzoylphenyl ureas as toxicants and chitin synthesis inhibitors in Oncopeltus fasciatus. Pestic. Biochem. Physiol. 11:33-45 https://doi.org/10.1016/0048-3575(79)90045-2
  11. Hama, H. (1987) Development of pyrethroid resistance in the diamondback moth, Plutella xylostella Linne (Lepidoptera:Yponomeutidae). Appl. Entomol. Zool. 22:166-175
  12. Joia, B.S., A.S. Udeaan and R.P. Chawla (1996) Toxicity of cartap hydrochloride and other insecticides to multi-resistant strains of the diamondback moth, Plutella xylostella (L.) in the Punjab. Int. Pest Control 38:158-159
  13. Kim, G.H., Y.S. Seo, J.H. Lee and K.Y. Cho (1990) Development of fenvalerate resistance in the diamondback moth, Plutella xylostella Linne (Lepidoptera: Yponomeutidae) and its cross resistance. Korean J. Appl. Entomol. 29:194-200
  14. Kim, G.H., Y.J. Ahn and K. Y. Cho (1992) Effect of diflubenzuron on longevity and reproduction of Riptortus clavatus (Hemiptera:Alydidae). J. Econ. Entomol. 85:664-668
  15. Kim, K.S., B.J. Chung and H.K. Kim (2000) Proceedings of the British Crop Protection Council Conference - Pest and Diseases 2000, Vol. 1, ed. by K.S. Kim, B.J. Chung and H.K. Kim: The British Crop Protection Council, Farnham, United Kingdom, pp. 41-46
  16. Kim M.K., H.Y. Kim, D.K. Seo, C. Yoon and G.H. Kim (2007) Insecticidal properties of bistrifluron, benzoylphenylurea insecticide, against Cotton caterpillar, Palpita indica (Lepidoptera: Pyralidae). J. Asia-Pacific Entomol. 10:269-274 https://doi.org/10.1016/S1226-8615(08)60362-3
  17. Liu, M.Y., Y.J. Tzeng and C.N. Sun (1982) Insecticide resistance in the diamondback moth. J. Econ. Entomol. 75:153-155
  18. Liu, M.Y., J.S. Chen and C.N. Sun (1984) Synergism of pyrethroids by several compounds in larvae of the diamondback moth (Lepidoptera: Plutellidae). J. Econ. Entomol. 77:851-856
  19. Mohan, M. and G.T. Gujar (2000) Susceptibility pattern and development of resistance in the diamondback moth, Plutella xylostella L, to Bacillus thuringiensis Berl var kurstaki in India. Pest Manag. Sci. 56:189-194 https://doi.org/10.1002/1526-4998(200002)56:2<189::AID-PS95>3.0.CO;2-T
  20. Noppun, V., T. Miyata and T. Saito (1984) Decrease in insecticide resistance in the diamondback moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae) on release from selection pressure. Appl. Entomol. Zool. 19:531-533
  21. Noppun, V., T. Miyata and T. Saito (1989) Cross resistance and synergism in fenvalerate-resistant diamondback moth, Plutella xylostella (L.). J. Pestic. Sci. 14:203-209 https://doi.org/10.1584/jpestics.14.203
  22. Oberlander, H., D.L. Silhacek, E. Shaaya and I. Ishaaya (1997) Current status and future perspectives of the use of insect growth regulators for the control of stored product insects. J. Stored prod. Res. 33:1-6 https://doi.org/10.1016/S0022-474X(96)00047-1
  23. Perng, F.S., M.C. Yao, C.F. Hung and C.N. Sun (1988) Teflubenzuron resistance in diamondback moth (Lepidoptera:Plutellidae). J. Econ. Entomol. 81:1277-1282
  24. Raffa, K.R. and T.M. Priester (1985) Synergist as research tools and control agents in agriculture. J. Agric. Entomol. 2:27-45
  25. Sarfraz, M., L.M. Dosdall, and B.A. Keddie (2006) Diamondback moth-host plant interactions: Implication for pest management. Crop Protection 25:625-639 https://doi.org/10.1016/j.cropro.2005.09.011
  26. SAS institute (2003) SAS, version 8.02. The SAS Institute, Cary, North Carolina, USA
  27. Song, S.S. (1991) Resistance of diamondback moth (Plutella xylostella L.: Yponomeutidae: Lepidoptera) against Bacillus thuringiensis Berliner. Korean J. Appl. Entomol. 30:291-293
  28. Sun, C.N., H. Chi and H.T. Feng (1978) Diamondback moth resistance to diazinon and methomyl in Taiwan. J. Econ. Entomol. 71:551-554
  29. Staal, G.B. (1975) Insect growth regulators with juvenile hormone activity. Annu. Rev. Entomol. 20:417-460 https://doi.org/10.1146/annurev.en.20.010175.002221
  30. Tabashnik, B.E., N.L. Cushing, N. Finson and M.W. Johnson (1990) Field development of resistance to Bacillus thuringiensis in diamondback moth (Lepidoptera: Plutellidae). J. Econ. Entomol. 83:1671-1676
  31. Yoon, C., J.O. Yang, S.H. Kang and G.H. Kim (2007) Insecticidal properties of bistrifluron against sycamore lace bug, Corythucha ciliata (Hemiptera: Tingidae). J. Pestic. Sci. 33:44-50 https://doi.org/10.1584/jpestics.G07-09