대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)

  • 제23권1호
  • /
  • Pages.1-22
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  • 2009
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  • 1226-8038(pISSN)
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  • 2287-9463(eISSN)
대한한방소아과학회 (The Association of Korean Oriental Pediatrics)
권호 표지

이중탕(理中湯)이 IgE가 과잉생성되고 피부염이 유발된 NC/Nga생쥐에서 $IFN-{\gamma}$ 반응에 의한 Th2 세포분화 억제에 미치는 영향

The Effect of Li Zhong Tang on the Suppression of Th2 Differentiation by $IFN-{\gamma}$ Response in IgE Hyperproduction and Atopic Dermatitis-like Skin Lesions Induced NC/Nga Mouse

  • 서희연 (대전대학교 한의과대학 소아과학교실) ;
  • 한재경 (대전대학교 한의과대학 소아과학교실) ;
  • 김윤희 (대전대학교 한의과대학 소아과학교실)
  • Seo, Hui-Yeon (Department of Pediatrics, College of Oriental Medicine, Daejeon University) ;
  • Han, Jae-Kyung (Department of Pediatrics, College of Oriental Medicine, Daejeon University) ;
  • Kim, Yun-Hee (Department of Pediatrics, College of Oriental Medicine, Daejeon University)
  • 발행 : 2009.04.30
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초록

Objectives The purpose of this study is to investigate the effect of Li Zhong Tang(LZH-T) on atopic dermatitis by using NC/Nga atopic dermatitis mouse. Methods First, in vitro, we isolated B cells from NC/Nga mouse which induced atopic dermatitis-like skin for 18 weeks. We analyzed FACS by intracellular staining of $IFN-{\gamma}$, GATA-3+ and also analyzed cytokines by using real-time PCR. Secondly, in vivo, after administration of LZH-T to the 12 weeks old mouse with atopic dermatitis. We analyzed serum IgE, $IFN-{\gamma}$ level and observed the changes of activated cell. Results In vitro, LZH-T decreased the levels of CD4+/$IFN-{\gamma}$ and increased the levels of CD4+/GATA3+. In vivo, serum IgE levels were decreased and $IFN-{\gamma}$ levels were increased in LZH-T group compared to the control group. In PBMCs, the percentage of activated cell - granulocytes, CD3+, CD3+CD4+, B220+CD23+, and CCR3+ were decreased and CD19+, CD3+CD8+ were increased in LZH-T group compared to the control group. Conclusions This study demonstrates immunological activity of GPJST on atopic dermatitis-like model mice.

키워드

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