게르마늄 강화 송이균사체의 항산화 및 항암 활성에 관한 연구

Study on Activities of Antioxidant and Anticancer of Germanium-fortified Tricholoma matsutake Myceliumm

  • 김혜자 (원광대학교 한의학전문대학원) ;
  • 김완겸 (원광대학교 한의학전문대학원) ;
  • 조화은 (원광대학교 한의학전문대학원) ;
  • 최윤희 (원광대학교 한의학전문대학원) ;
  • 이기남 (원광대학교 한의학전문대학원) ;
  • 정명수 (원광대학교 한의과대학)
  • Kim, Hae-Ja (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim, Wan-Gyeom (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Cho, Hwa-Eun (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Choi, Yun-Hee (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Ki-Nam (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Chong, Myong-Soo (College of Oriental Medicine, Wonkwang University)
  • 발행 : 2009.04.25

초록

The purpose of this study was evaluated physiological activity effect of organic germanium in culture broth of germanium-fortified Tricholoma matsutake mycelium and germanium-fortified yeast. Proliferation Tricholoma matsutake mycelium and yeast was inhibited by addition of germanium. Contents of organic germanium in Tricholoma matsutake mycelium and yeast was increased in dose-dependent manner. And low concentration(1,000 ppm) of germanium in mycelium was almost changed organic germanium. In the result of antioxidant activity as SOD-like activity, contents of total polyphenol compound and electron donating ability, activity of germanium-fortified Tricholoma matsutake mycelium was higher than that of germanium-fortified yeast. To evaluate of antitumor effects in vitro, we examined nitric oxide production of Raw 264,7 cell and cytotoxicity of HT1080 cell by MTT assay. Nitric oxide production of germanium-fortified Tricholoma matsutake mycelium was shown low level in low concentration(1,000 ppm) than other groups. The anticancer effect of germanium-fortified Tricholoma matsutake mycelium on HT 1080 cell was indicated a strong inhibitory effect in low concentration(1,000 ppm). These results suggest that organic germanium in culture broth of germanium-fortified Tricholoma matsutake mycelium has valuable physiological activities as antioxidant and anticancer effect, and it was higher than that of germanium-fortified yeast.

키워드

참고문헌

  1. Lee, J.S., Park, J.L., Kim, S.H., Park, S.H., Kang, S.K., Park, C.B., Sohn, T.U., Jang, J.Y., Kang, J.K., Kim, Y.B. Oral Single and repeated-dose toxicity studies on Geranti Bio-Ge yeast, organic germanium fortified yeasts, in rats. J Toxicol sci 29: 541-553, 2004 https://doi.org/10.2131/jts.29.541
  2. Sandra, G. Therapeutic effect of organic germanium. Medical Hypotheses 26: 207-215, 1988 https://doi.org/10.1016/0306-9877(88)90101-6
  3. Lee, H.K., Kim, J.S., Kang, T.B. Extraction of organic germanium compound from garlic. Inst Natural Sci Sangmyung Univ 12: 1-18, 2004
  4. Schroeder, H.A., Balassa, J.J. Arsenic germanium, tin and vanadium in mice: Effects on growth, survival and tissue levels. J Nutr 92: 297-316, 1967
  5. Gadd, G.M. Microbial formation and transformation of organometallic and organometalloid compounds. FEMS Microbiology Review 11: 297-316, 1993 https://doi.org/10.1111/j.1574-6976.1993.tb00003.x
  6. Nobohiro, W., Osamu, I., Takuro, K., Koichi, Y. New approaches to using spent brewer's yeast. ASBC journal 38: 5-8, 1980
  7. Wei, X.S. Effects of yeast on bioenrichment of germanium. Food science 149: 49-54, 1992
  8. Klapcinska, B., Chmielowski, J. Binding of germanium to Pseudomonas putida cells. Appl Enuiron Microbial 51: 1144-1147, 1986
  9. Lynn, M.R., Geoffrey, M.G. Mutants of Saccharomyces cerevisiae defective in vacuolar function a role for the vacuole in toxic metal ion detoxification. Microbiol Lett 152: 293-298, 1997 https://doi.org/10.1111/j.1574-6968.1997.tb10442.x
  10. Korean Forestry Reports, Reports on the pine musbroom in Korea. Forest Research Institute, Seoul, Korea. 18: 44-46, 1981
  11. An, J.S., Lee, K.H. Studies on the mineral contents of Korean mushroom. Korean J Food Hygime and Safety 1(2):177-179, 1986
  12. Lee, S.T., Lee, Y.H., Choi, Y.J., Lee, S.D., Lee, C.H., Heo, J.S. Growth characteristics and germanium absorption of rice plant with different germanium concentration in soil. Korean J Environ Agric 24(1):40-44, 2005 https://doi.org/10.5338/KJEA.2005.24.1.040
  13. Swain, T., Hillis, W.E., Otega, M. Phenolic constituents of Prunus domestica. I. Quantitative analysis of phenolic constituents. J Sci Food Agric 10: 83-88, 1959
  14. Blois, M.S. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200, 1958 https://doi.org/10.1038/1811199a0
  15. Lee, B.G., Kim, S.H., Zee, O.P., Lee, K.R., Lee, H.Y., Han, J.W., Lee, H.W. Suppression of inducible nitric oxide synthase expression in Raw 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach. Eur Pharmacol 406: 301-309, 2000 https://doi.org/10.1016/S0014-2999(00)00680-4
  16. Goodman, S. Therapeutic effects of organic germanium. Medical hypothesis 26: 207-215, 1988 https://doi.org/10.1016/0306-9877(88)90101-6
  17. Arimori, S., Watanabe, K., Yoshida, M., Nagato, T. Effect of Ge132 products and related synthetic compounds. Amsterdam ISBN Elsevier Science, pp 536-539, 1981
  18. Bigitta, E.L., Mann, H., Melzer, H., Dunemann, L., Begerow, J. Renal and other failure caused by germanium Intoxication. Nephrol Dial Translant 14: 2464-2468, 1999 https://doi.org/10.1093/ndt/14.10.2464
  19. Tao, S.H., Bolger, P.M. Hazard assessment of germanium supplements. Regulatory Toxicology and pharm acology 25: 211-219, 1997 https://doi.org/10.1006/rtph.1997.1098
  20. Yamada, H., Kiyohara, H., Cyong, J.C., Otsuka, Y. Purification and characterization of complement activating acidic polysaccharides from the roots of Lithospermum euchromum royale. International J Immunopharmacology 8: 71-82, 1986 https://doi.org/10.1016/0192-0561(86)90075-5
  21. Park, K.M., Kim, Y.S., Jeong, T.C., Joe, C.O., Shin, H.J., Lee, Y.H., Nam, K.Y., Park, J.D. Nitric oxide is involved in the immunomodulating activities of acidic polysaccharide from Panax ginseng. Planta Medica 67: 122-126, 2001 https://doi.org/10.1055/s-2001-11508
  22. Park, J.D. Recent studied on the chemical constituents of Korean ginseng(Panax ginseng CA Meyer). Korean J Ginseng Sci 20: 389-396, 1996