동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제23권2호
  • /
  • Pages.473-479
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  • 2009
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

게르마늄 강화 송이균사체의 항산화 및 항암 활성에 관한 연구

Study on Activities of Antioxidant and Anticancer of Germanium-fortified Tricholoma matsutake Myceliumm

  • 김혜자 (원광대학교 한의학전문대학원) ;
  • 김완겸 (원광대학교 한의학전문대학원) ;
  • 조화은 (원광대학교 한의학전문대학원) ;
  • 최윤희 (원광대학교 한의학전문대학원) ;
  • 이기남 (원광대학교 한의학전문대학원) ;
  • 정명수 (원광대학교 한의과대학)
  • Kim, Hae-Ja (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim, Wan-Gyeom (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Cho, Hwa-Eun (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Choi, Yun-Hee (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Ki-Nam (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Chong, Myong-Soo (College of Oriental Medicine, Wonkwang University)
  • 발행 : 2009.04.25
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초록

The purpose of this study was evaluated physiological activity effect of organic germanium in culture broth of germanium-fortified Tricholoma matsutake mycelium and germanium-fortified yeast. Proliferation Tricholoma matsutake mycelium and yeast was inhibited by addition of germanium. Contents of organic germanium in Tricholoma matsutake mycelium and yeast was increased in dose-dependent manner. And low concentration(1,000 ppm) of germanium in mycelium was almost changed organic germanium. In the result of antioxidant activity as SOD-like activity, contents of total polyphenol compound and electron donating ability, activity of germanium-fortified Tricholoma matsutake mycelium was higher than that of germanium-fortified yeast. To evaluate of antitumor effects in vitro, we examined nitric oxide production of Raw 264,7 cell and cytotoxicity of HT1080 cell by MTT assay. Nitric oxide production of germanium-fortified Tricholoma matsutake mycelium was shown low level in low concentration(1,000 ppm) than other groups. The anticancer effect of germanium-fortified Tricholoma matsutake mycelium on HT 1080 cell was indicated a strong inhibitory effect in low concentration(1,000 ppm). These results suggest that organic germanium in culture broth of germanium-fortified Tricholoma matsutake mycelium has valuable physiological activities as antioxidant and anticancer effect, and it was higher than that of germanium-fortified yeast.

키워드

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