생명과학회지 (Journal of Life Science)

  • 제19권4호
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  • Pages.467-471
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  • 2009
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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돼지 mtDNA D-loop 지역의 Large White 특이 중복현상 탐지

Detection of a Large White-Specific Duplication in D-loop Region of the Porcine MtDNA

  • 김재환 (농촌진흥청 국립축산과학원) ;
  • 한상현 (농촌진흥청 국립축산과학원) ;
  • 이성수 (농촌진흥청 국립축산과학원) ;
  • 고문석 (농촌진흥청 국립축산과학원) ;
  • 이정규 (경상대학교 응용생명과학부) ;
  • 전진태 (경상대학교 응용생명과학부) ;
  • 조인철 (농촌진흥청 국립축산과학원)
  • Kim, Jae-Hwan (National Institute of Animal Science, R. D. A.) ;
  • Han, Sang-Hyun (National Institute of Animal Science, R. D. A.) ;
  • Lee, Sung-Soo (National Institute of Animal Science, R. D. A.) ;
  • Ko, Moon-Suk (National Institute of Animal Science, R. D. A.) ;
  • Lee, Jung-Gyu (Division of Applied Life Science, Gyeongsang National University) ;
  • Jeon, Jin-Tae (Division of Applied Life Science, Gyeongsang National University) ;
  • Cho, In-Cheol (National Institute of Animal Science, R. D. A.)
  • 발행 : 2009.04.30
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초록

돼지 6품종(Landrace, Duroc, Large White, 한국재래돼지, Berkshire, Hampshire)을 대상으로 기존에 보고된 서열을 바탕으로 제작한 primer를 이용하여 mtDNA D-loop 전체영역을 증폭하였다. 증폭된 PCR product를 cloning 및 DNA sequencing, 다중염기서열비교를 통하여 분석한 결과, mtDNA에서 heteroplasmy가 나타나는 D-loop 내 tandem repeat region 이후에 11-bp 중복이 존재하는 것을 확인하였다. 이런 중복현상은 일본재래돼지와 Duroc에서 보고되었지만, 이를 이용한 돼지 품종별 중복현상의 빈도 및 분포에 관한 연구는 이루어져있지 않다. 품종별 11-bp 중복현상을 분석하기 위해서 6품종을 대상으로 중복지역을 포함한 약 150 bp 절편을 증폭하였으며, PAGE 방법을 통하여 분석하였다. 그 결과 본 연구에서 사용한 품종들 중 모든 Large White에서 중복현상이 발생하는 것을 확인하였으며, Duroc인 경우 11.2% (9/80)에서 중복현상이 확인되었다. 반면에 Landrace, 한국재래돼지, Berkshire 및 Hampshire에서는 전혀 발견되지 않았다. 이런 결과로서, 11-bp 중복현상의 분석은 현재 구별이 불가능한 Landrace와 Large White를 구별할 수 있는 유용한 DNA marker로서 사용이 가능할 것이다.

The entire D-loop region of the porcine mitochondrial DNA (mtDNA) was amplified from six pig breeds (Landrace, Duroc, Large White, Korean native pig, Berkshire, and Hampshire) using a primer set designed on the basis of reported porcine mtDNA sequences. From analyses through cloning, DNA sequencing and multiple sequence alignment, an 11-bp (TAAAACACTTA) duplication was observed after known tandem repetition in the D-loop region, which promoted hetroplasmy in mtDNA. Although the existence of the 11-bp duplication has been previously reported in Duroc and Japanese native pigs, there have not been any attempts to know the characteristics of this duplication in other breeds so far. A 150 bp fragment containing the 11-duplication was amplified and typed by polyacrylamide gel electrophoresis (PAGE). All Large Whites had two duplication units and Duroc showed heteromorphic patterns, 11.2% (9/80) of the animals had the 11-bp duplication in total. On the other hand, Landrace, Berkshire, Hampshire and Korean native pigs were non-duplicated. This result showed that the 11-bp duplication could be used as a breed-specific DNA marker for distinguishing pure Landrace and Large White breeds.

키워드

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