생명과학회지 (Journal of Life Science)

  • 제19권4호
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  • Pages.462-466
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  • 2009
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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오만둥이의 항산화 및 TNF-α 유도 활성

Antioxidant and TNF-α Inducing Ability of Styela plicata Extracts

  • 서현효 (국립진주산업대학교 환경공학과) ;
  • 이보배 (경남대학교 식품생명학과) ;
  • 이승철 (경남대학교 식품생명학과)
  • Suh, Hyun-Hyo (Department of Environmental Engineering, Jinju National University) ;
  • Lee, Bo-Bae (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University)
  • 발행 : 2009.04.30
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초록

메탄올, 에탄올, 아세톤 그리고 물을 이용하여 신선한 오만둥이의 추출물을 제조한 후, 추출물의 항산화능 및 $TNF-{\alpha}$ 유도능을 조사하였다. 아세톤 추출물을 제외한 대부분의 추출물은 malonedialdehyde 생성을 50% 이상 저해하는 항산화능을 보였으며, 메탄올 추출물은 가장 높은 값인 59.3%의 malonedialdehyde 생성 저해능을 나타내었다. 물과 아세톤 추출물은 각각 18.5와 $12.3\;{\mu}M$의 glutathione 함량을 보였지만 그 외의 추출물에서는 검출되지 않았다. 추출물들의 $TNF-{\alpha}$ 유도능을 RAW 264.7 세포주로 조사하였다. 오만둥이 물 추출물은 첨가량이 증가할수록 $TNF-{\alpha}$ 생성을 증가시켰다. 그러나 그 이외의 추출물들은 RAW 264.7 세포주에서의 증가할수록 $TNF-{\alpha}$ 생성을 유도하지 못하였다.

After preparing extracts from fresh Styela plicata using methanol, ethanol, acetone, and water, the antioxidant and tumor necrosis factor $(TNF)-{\alpha}$ inducing ability of the extracts were evaluated. Most of the extracts showed more than 50% inhibitory activity against malonedialdehyde formation, and methanol extract exhibited the highest activity with values of 59.3%. The water and acetone extracts contained glutathione contents of 18.5 and $12.3\;{\mu}M$, respectively, however, these were not detected in the other extracts. $TNF-{\alpha}$ inducing abilities of the extracts were determined with RAW 264.7 cells. The water extract increased $TNF-{\alpha}$ production with correlation to increase of the added amount. However, the other extracts could not induce $TNF-{\alpha}$ production in RAW 264.7 cells. The results indicated that the antioxidant activities of S. plicata extracts were different depending on extracting solvents, and the water extract showed significant $TNF-{\alpha}$ inducing activity.

키워드

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피인용 문헌

  1. Antioxidant and Antihypertensive Activities of Styela plicata according to Harvesting Time and Size vol.40, pp.3, 2011, https://doi.org/10.3746/jkfn.2011.40.3.350
  2. A hexameric peptide purified from Styela plicata protects against free radical-induced oxidative stress in cells and zebrafish model vol.6, pp.59, 2016, https://doi.org/10.1039/C6RA06308J
  3. Effect of angiotensin I-converting enzyme (ACE) inhibitory peptide purified from enzymatic hydrolysates of Styela plicata vol.233, pp.6, 2011, https://doi.org/10.1007/s00217-011-1585-7