Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Enhancement of cadmium resistance by overexpression of BrMT3 in Arabidopsis

BrMT3 고발현에 의한 애기장대의 카드뮴 저항성 증진

  • Kim, Sun-Ha (Department of Applied Biology, Chungnam National University) ;
  • Song, Won-Yong (Department of Applied Biology, Chungnam National University) ;
  • Ahn, Young-Ock (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Haeng-Soon (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwak, Sang-Soo (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Choi, Kwan-Sam (Department of Applied Biology, Chungnam National University)
  • 김선하 (충남대학교 농업생명과학대학 대학원) ;
  • 송원용 (충남대학교 농업생명과학대학 대학원) ;
  • 안영옥 (한국생명공학연구원 환경바이오 연구센터) ;
  • 이행순 (한국생명공학연구원 환경바이오 연구센터) ;
  • 곽상수 (한국생명공학연구원 환경바이오 연구센터) ;
  • 최관삼 (충남대학교 농업생명과학대학 대학원)
  • Published : 2009.03.31
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Abstract

We have previously demonstrated that overexpression and characterization of Brassica rapa type-l metallothionein gene (BrMT1) in Arabidopsis which showed enhanced resistance to cadmium and ROS. Here, we present the consistent study of our previous report about BrMTs. BrMT3 expressing DTY167 cells showed resistance to Zn and Pb as well as Cd. Thus, we have developed the BrMT3 overexpression Arabidopsis to enhance capacity for metal stresses. Successful expression and localization were achieved using the rubisco transit peptides of RbcS-BrMT3-GFP protein, which was confirmed by western blot analysis with the GFP antibody and green fluorescence signal from the chloroplast. BrMT3 overexpression Arabidopsis plants exhibited a higher resistance to cadmium compared to control plants. This result indicates that BrMT3 would be applicable to the development of plants with enhanced resistance against heavy metal stresses.

B. rapa로부터 분리한 BrMT3 유전자를 도입시킨 효모와 애기장대가 카드뮴을 비롯한 중금속에 저항성을 보이는 것이 확인되었고 이 결과를 토대로 이 유전자가 중금속 흡착을 통한 환경 정화 및 스트레스에 내성을 갖는 형질전환 식물체를 개발하는데 유용하게 사용될 것으로 기대된다.

Keywords

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