Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Identification of another calmodulin-binding domain at the C-terminal region of AtCBP63

  • Kim, Sun-Ho (Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Kang, Yun-Hwan (Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Han, Hay-Ju (Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Bae, Dong-Won (Central Instrument Facility, Gyeongsang National University) ;
  • Kim, Min-Chul (Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Lim, Chae-Oh (Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Chung, Woo-Sik (Environmental Biotechnology National Core Research Center and Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
  • Published : 2009.03.31
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Abstract

Calcium signals can be transduced by binding calmodulin (CaM), a $Ca^{2+}$ sensor in eukaryotes, is known to be involved in the regulation of diverse cellular functions. We isolated a CaM-binding protein 63 kD (AtCBP63) from the pathogen-treated Arabidopsis cDNA expression library. Recently, AtCBP63 was identified as a CaM bining protein. The CaM binding domain of AtCBP63 was reported to be located in its N-terminal region, In this study, however, we showed that ACaM2 could specifically bind to second CaM-binding domain (CaMBD) of AtCBP63 at the C-terminal region. The specific binding of CaM to CaM binding domain was confirmed by a gel mobility shift assay, a split ubiquitin assay, site-directed mutagenesis, and a competition assay using a $Ca^{2+}$/CaM-dependent enzyme. The gene expression of AtCBP63 was induced by pathogens and pathogens related second messengers. This result suggests that a CaM binding protein, AtCBP63, may play role in pathogen defense signaling pathway.

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