Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Genome Wide Expression Profile of Agrimonia pilosa in LPS-stimulated BV-2 Microglial Cells

  • Sohn, Sung-Hwa (BK21 Oriental Medical Science Center, KyungHee University) ;
  • Ko, Eun-Jung (BK21 Oriental Medical Science Center, KyungHee University) ;
  • Kim, Sung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, KyungHee University) ;
  • Kim, Yang-Seok (BK21 Oriental Medical Science Center, KyungHee University) ;
  • Shin, Min-Kyu (BK21 Oriental Medical Science Center, KyungHee University) ;
  • Hong, Moo-Chang (BK21 Oriental Medical Science Center, KyungHee University) ;
  • Bae, Hyun-Su (BK21 Oriental Medical Science Center, KyungHee University)
  • Published : 2009.03.31
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Abstract

Microglial cells constitute the first line of defense against infection and injury in the brain. This study was conducted to evaluate the protective mechanisms of Agrimonia pilosa (AP) on LPS-induced activation of BV-2 microglial cells. The effects of AP on gene expression profiles in activated BV-2 microglial cells were evaluated using microarray analysis. BV-2 microglial cells were cultured in a 100 mm dish ($1{\times}10^7/mL$) for 24 hr and then pretreated with 1 g/mL AP or left untreated for 30 min. Next, 1 g/mL LPS was added to the samples and the cells were reincubated at $37^{\circ}C$ for 30 min, 3 hr and 6 hr. The gene expression profiles of the BV-2 microglial cells varied depending on the AP. The microarray analysis revealed that MAPK signaling pathway-related genes were down-regulated and IL10 gene was up-regulated in AP-treated BV-2 microglial cells. AP can affect the inflammatory response and MAPK pathway in BV-2 microglial cells.

Keywords

References

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