Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Microtubule Assemblies and Methylation Patterns of Porcine IVF and Parthenogenetic Embryos

돼지 체외수정란 및 단위발생란의 미세관 형성 및 메틸화 양상

  • Park, Joo-Hee (School of Veterinary Medicine, Kangwon National University) ;
  • Kim, Ho-Jeong (School of Veterinary Medicine, Kangwon National University) ;
  • Lee, Beom-Ki (School of Veterinary Medicine, Kangwon National University) ;
  • Kwon, Dae-Jin (School of Veterinary Medicine, Kangwon National University) ;
  • Hwang, In-Sun (College of Animal Life Science, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Science, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Science, Kangwon National University) ;
  • Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University)
  • 박주희 (강원대학교 수의학부대학) ;
  • 김호정 (강원대학교 수의학부대학) ;
  • 이범기 (강원대학교 수의학부대학) ;
  • 권대진 (강원대학교 수의학부대학) ;
  • 황인선 (강원대학교 동물생명과학대학) ;
  • 박춘근 (강원대학교 동물생명과학대학) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의학부대학)
  • Published : 2009.03.31
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Abstract

This study was performed to confirm the microtubule assemblies and methylation patterns of porcine IVF and parthenogenetic embryos. Cumulus-oocyte complexes were collected and matured in vitro for 42 hr. Oocytes were fertilized by prepared fresh sperm or activated parthenogenetically by exposure to electric stimulation and 6-dimethylaminopurine. Porcine IVF and parthenogenetic embryos were cultured in vitro for 6 days. Embryos were stained by immunofluorescence staining method to observe the dynamic of nucleus and microtubules in the first mitotic phase and the methylation patterns in different developmental stages. After then, samples were confirmed and analyzed through a laser-scanning confocal microscope. IVF embryos had a centrosome originated from sperms, which was shown a $\gamma$-tubulin spot. However, $\gamma$-tubulin spot was not observed in parthenogenetic embryos. A lower methylation level was observed in IVF embryos compared to parthenogenetic ones at the morula and blastocyst stages. In conclusion, it is considered that microtubule assemblies and genetic regulation mechanism differ between parthenogenetic and IVF embryos.

Keywords

References

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