Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Rosmarinus officinalis L. Fractions on Antimicrobial Activity against Methicillin-resistant Staphylococcus aureus (MRSA) and Resistant Genes Regulation

로즈마리(Rosmarinus officinalis L.) 추출물의 항생제 내성균주(MRSA)에 대한 항균활성 및 내성 유전자 조절 효과

  • Choi, Jun-Hyeok (Dept. of Food Science and Technology, Keimyung University) ;
  • Yu, Mi-Hee (Dept. of Food Science and Technology, Keimyung University) ;
  • Hwang, Eun-Young (Dept. of Food Science and Technology, Keimyung University) ;
  • Lee, In-Seon (The Center for Traditional Microorganism Resources, Keimyung University)
  • 최준혁 (계명대학교 식품가공학) ;
  • 유미희 (계명대학교 식품가공학) ;
  • 황은영 (계명대학교 식품가공학) ;
  • 이인선 (계명대학교 전통미생물 자원 개발 및 산업화 연구 센터)
  • Published : 2009.05.29
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Abstract

This study was performed to evaluate antimicrobial activity of methanol extract from Rosmarinus officinalis L. and their fractions on methicillin-resistant Staphylococcus aureus (MRSA) and resistant gene regulation. The methanol extract of Rosmarinus officinalis L. and their hexane fractions showed the strongest antimicrobial activity against S. aureus and MRSA. To observe the morphological change of MRSA according to the hexane fraction $80{\mu}g$/mL treatment, scanning electron microscope (SEM) of MRSA were measured. The results from SEM showed decreased number of bacteria, lysis and damaged cell wall. Expressions of MecA and penicillinase were substantially decreased in a dose-dependent manner on MRSA that had been treated with methanol extract of Rosmarinus officinalis L. and their hexane fractions.

42가지의 천연물 시료 중 세신, 호두(열수), 골쇄보, 뽕잎, 로즈마리에서 S. aureus ATCC 25923 및 MRSA KCCM 40511에 대해 우수한 항균활성을 나타냈다. 이들 시료 중 가장 우수한 항균활성을 가지는 로즈마리를 순차 분획하여 각 분획물을 이용한 MRSA에 대한 항균활성을 알아본 결과 hexane, chloroform 분획물에서 항균활성을 나타냈으며, 각 분획물 중 hexane 분획물이 가장 높은 저해활성을 나타내었다. 또한 SEM을 통한 형태학적 관찰을 통해 분획물 처리 시 MRSA의 세포벽이 파괴되는 것을 확인할 수 있었다. 로즈마리 메탄올추출물 및 hexane 분획물은 MRSA KCCM 40511의 내성 단백질인 MecA와 MecA에 의해 합성되는 세포벽 단백질 penicillinase의 발현을 억제시킴으로써 고도 내성균의 내성을 억제시키는 것으로 나타났다.

Keywords

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