볏붉은잎 추출물의 돼지 유래 병원성 세균에 대한 항균효과

Antibacterial activity of Callophyllis japonica-methanol extracts against the pathogenic bacteria from swine

  • 정진우 (제주특별자치도 동물위생시험소) ;
  • 정찬우 (제주특별자치도 동물위생시험소) ;
  • 김정태 (제주대학교 수의과대학) ;
  • 양원준 (제주대학교 수의과대학) ;
  • 안미정 (제주대학교 수의과대학) ;
  • 김병학 (제주특별자치도 동물위생시험소) ;
  • 김주아 (제주특별자치도 동물위생시험소) ;
  • 신태균 (제주대학교 수의과대학)
  • Jeong, Jin-Woo (Jeju Veterinary Research Institute, Jeju Special Self-Governing Province) ;
  • Jeong, Chan-Woo (Jeju Veterinary Research Institute, Jeju Special Self-Governing Province) ;
  • Kim, Jeong-Tae (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Yang, Won-Joon (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Ahn, Mee-Jung (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Kim, Byeoung-Hak (Jeju Veterinary Research Institute, Jeju Special Self-Governing Province) ;
  • Kim, Joo-Ah (Jeju Veterinary Research Institute, Jeju Special Self-Governing Province) ;
  • Shin, Tae-Kyun (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • 발행 : 2009.12.30

초록

Interest in marine organisms as potential sources of bioactive agents has increased in recent years. The red seaweed, Callophyllis (C.) japonica, is abundant in the coastal regions of Jeju Island in South Korea. A previous study shows that C. japonica extracts have antioxidant activity and radioprotective effects. In this study, an methanol extract of C. japonica was tested whether it has antibacterial effects against the bacteria from swine. In vitro antibacterial activities of the crude extracts prepared from the C. japonica using 80 % methanol were tested for inhibitory activity against the Escherichia (E.) coli (S175), Enterococcus (E.) faecium (ATCC 51558), Salmonella (S.) Typhimurium and Staphylo-coccus (S.) aureus (ATCC 25923) by using broth dilution method. All organisms were incubated in brain heart infusion medium containing 1% extract at 0, 4, 8, 12 and 24 hrs. The 3 days-old piglets were fed an experimental diet supplemented with 1% C. japonica for 1 week. And the change of the coliform bacteria in feces were examined after supplement of C. japonica for 1 week. When the inocula containing $10^2{\sim}10^3$CFU/ml of each organism were used the extracts of C. japonica showed various degrees of antibacterial effects on all bacteria tested. The CFU value ($6.3\times10^8$CFU/ml) of C. japonica for E. coli was decreased 30% compared with vehicle controls ($9.0\times10^8$CFU/ml) after 8 hrs incubation. The proliferation rate of E. faecium was inhibited about 68% at 4 hrs, 81% at 8 hrs and 76% at 12 hrs after incubation, respectively. The proliferation rate of S. Typhimurium was inhibited about 96% at 4 hrs, 90% at 8 hrs and 72% at 12 hrs after incubation with extracts of C. japonica. The proliferation rate of S. aureus was inhibited more than 90% each time courses. Conclusively, a red seaweed extract of C. japonica was found to be effective against a number of gram negative and gram positive bacteria such as E. coli, E. faecium, S. Typhimurium, and S. aureus. The number of coliform bacteria was increased in the 1% C. japonica-treated group, as compared to those of controls. This result suggests that C. japonica extracts be added as an effective natural antibacterial agent. The precise mechanism of antibacterial effects and its application on swine industry remains to be further studied.

키워드

참고문헌

  1. 김진아, 이종미. 2004. 건조방법에 따른 해조류(톳)의 생리활성 성분 및 항산화 활성의 변화. 한국식생활문화학회지 19(2): 200-208
  2. 이학성, 서정호, 서근학. 2000. 해조류 추출물로부터 항균제의 제조 및 항균효과. 한국수산학회지 33(1): 32-37
  3. 임치원, 이종수, 송광태, 박정흠, 조영제. 1999. 참보라색우무 추출물의 항균특성. 국립수산과학원연구보고 57: 211-220
  4. 임치원, 이종수, 조영제. 2000. 참보라색우무에서 추출한 항균물질의 구조 및 특성. 한국수산학회지 33(4):280-287
  5. 홍의철, 김인호. 2001. 환경친화형 양돈사료를 위한 항생제 대체 기능성 물질. 한국유기농업학회지 9(4):136-149
  6. Aarestrup FM, Hasman H. 2004. Susceptibility of different bacterial species isolated from food animals to copper sulphate, zinc chloride and antimicrobial substances used for disinfection. Vet Microbiol 100(1-2): 83-89 https://doi.org/10.1016/j.vetmic.2004.01.013
  7. Bentley R, Meganathan R. 1982. Biosynthesis of Vitamin K (menaquinone) in Bacteria. Microbiol Rev 46(3): 241-280
  8. Cooper DM, Swanson DL, Gebhart CJ. 1997. Diagnosis of proliferative enteritis in frozen and formalin-fixed, paraffin-embedded tissues from a hamster, horse, deer and ostrich using a Lawsonia intracellularis-specific multiplex PCR assay. Vet Microbiol 54: 47-62 https://doi.org/10.1016/S0378-1135(96)01264-3
  9. Hays VW, Muir WM. 1979. Efficiency and safety of feed additive use of antibacterial drugs in animal production. Can J Anim Sci 59: 447-456 https://doi.org/10.4141/cjas79-055
  10. Hudault S, Guignot J, Servin AL. 2001. Escherichia coli strains colonizing the gastrointestinal tract protect germfree mice against Salmonella typhimurium infection. Gut 49(1): 47-55
  11. Kang KA, Bu HD, Park DS, Go GM, Jee Y, Shin T, Hyun JW. 2005. Antioxidant activity of ethanol extract of Callophyllis japonica. Phytother Res 19: 506-510 https://doi.org/10.1002/ptr.1692
  12. Kim J, Moon C, Kim H, Jeong J, Lee J, Kim J, Hyun JW, Park JW, Moon MY, Lee NH, Kim SH, Jee Y, Shin T. 2008. The radioprotective effects of the hexane and ethyl acetate extracts of Callophyllis japonica in mice that undergo whole body irradiation. J Vet Sci 9(3):281-284 https://doi.org/10.4142/jvs.2008.9.3.281
  13. Lim SB, Kim SH, Ko YH, Oh MC, Oh CG, Ko YG, Park JS. 1995. Extraction yields of Hizikia fusiformis and Aloe vera linne by supercritical carbon dioxide and antimicrobial activity of their extracts. Korean J Food Sci Technol 27(1): 68-73
  14. Mayer AM, Rodriguez AD, Berlinck RG, Hamann MT. 2009. Marine pharmacology in 2005~2006: marine compounds with anthelmintic, antibacterial, anticoagulant, antifungal, anti-inflammatory, antimalarial, antiprotozoal, antituberculosis, and antiviral activities : effecting the cardiovascular, immune and nervous systems, and other miscellaneous mechanisms of action. Biochim Biophys Acta 1790: 283-308 https://doi.org/10.1016/j.bbagen.2009.03.011
  15. Nagayama K, Iwamura Y, Shibata T, Hirayama I, Nakamura T. 2002. Bactericidal activity phlortannins from the brown alga Ecklonia kurome. J Antimicrob Chemother 50: 889-893 https://doi.org/10.1093/jac/dkf222
  16. Negi PS, Jayaprakasha GK, Jagan Mohan Rao L, Sakariah KK. 1999. Antibacterial Activity of Tumeric Oil: A Byproduct from Curcumin Manufacture. J Agric Food Chem 47: 4297-4300 https://doi.org/10.1021/jf990308d
  17. Park DS, Lee KH, Kim HC, Ahn MJ, Moon C, Ko MS, Lee KK, Go GM, Shin T. 2005. Effects of Callophyllis japonica powder on carbon tetrachloride-induced liver injury in rats. Orient Pham Exp Med 5: 231-235 https://doi.org/10.3742/OPEM.2005.5.3.231
  18. Reid G, Howard J, Gan BS. 2001. Can bacterial interference prevent infection? Trends Microbiol 9(9): 424-428 https://doi.org/10.1016/S0966-842X(01)02132-1
  19. Salmon SA, Watts JL, Case CA, Horrman LJ, Wegener HC, Yancey RJ Jr. 1995. Comparison of MICs of ceftiofur and other antimicrobial agents against bacterial pathogens of swine from the united states, canada, and denmark. J Clin Microbiol 33(9): 2435-2444
  20. Theppangna W, Murase T, Tokumaru N, Chikumi K, Shimizu E, Otsuki K. 2007. Screening of the enterocin genes and antimicrobial activity against pathogenic bacteria in Enterococcus strains obtained from different origins. J Vet Med Sci 69(12): 1235-1239 https://doi.org/10.1292/jvms.69.1235
  21. Vallinayagam K, Arumugam R, Kannan RR, Thirumaran G, Anatharaman. 2009. Antibacterial activity of some selected seaweeds from pudumadam. Global J Pharmacol 3(1): 50-52
  22. Wells JE, Oliver WT, Yen JT. 2009. The effect of dietary additives on faecal levels of Lactobacillus spp., coliforms, and Escherishia coli, and faecal prevalence of Salmonella spp. and Camphylobacter spp. in US production nursery swine. J Appl Microbiol 107: 1-9 https://doi.org/10.1111/j.1365-2672.2009.04143.x