탕약의 실온과 냉장보관 및 기간별 안정성에 대한 실험적 연구

Stability Study for Herbal Drug According to Storage Conditions and Periods

  • 손진영 (대전대학교 한의과대학 간장면역학 교실) ;
  • 신장우 (대전대학교 한의과대학 간장면역학 교실) ;
  • 손창규 (대전대학교 한의과대학 간장면역학 교실)
  • Son, Jin-Young (Internal Medicine & Immune Center, Daejeon University) ;
  • Shin, Jang-Woo (Internal Medicine & Immune Center, Daejeon University) ;
  • Son, Chang-Gue (Internal Medicine & Immune Center, Daejeon University)
  • 발행 : 2009.03.31

초록

Objectives: This study aimed to investigate the stability of a decoction using three herbal plants and their major components according to the storage conditions and periods. Materials and Methods: A three-herb mixture (1:1:1) of Glycyrrhiza uralensis Fischer, Artemisia capillaris Thunberg, and Poncirus trifoliata Rafinesqui was decocted and kept at room temperature ($25{\pm}2^{\circ}C$) or cold temperature ($4^{\circ}C$) for 0, 2, 4 or 8 weeks in liquid form in a plastic pack under dark conditions. At time points given, they were lyophilized. 200 mg of powdered samples were dissolved in 1 mL of 90% methanol and then applied to a high performance thin layer chromatography (HPTLC) with glycyrrhizin, 6,7-dimethoxycoumarin or poncirin for quantitative or qualitative analysis. Results: There were no gross changes in HPTLC-based compositional band-patterns of the three herbal mixture according to the storage conditions and period. The major components of each herb, glycyrrhizin, 6,7-dimethoxycoumarin and poncirin, showed slight time-dependent reduction in their contents both at room and cold temperature for 8 weeks. Conclusion: We could conclude that the current herbal decoction is generally safe for the stability at both RT or CT for at least 8 weeks. Nevertheless, we proposed that further advanced studies are required for more multiple herbal mixtures and longer storage periods.

키워드

참고문헌

  1. Jang IS, Yang CS, Lee SD, Han CH. A Review of Herbal Medicinal Products Associated with Toxic Events in Korea. J Korean Oriental Med.2007;28(1):1-10.
  2. Man SC, Durairajan SS, Kum WF, Lu JH, Huang JD, Cheng CF, Chung V, Xu M, Li M. Systematic review on the efficacy and safety of herbal medicines for Alzheimer's disease. Journal of Alzheimer's Disease. 2008;14(2):209-23.
  3. Kim TK, Jung WS, Park SW, Cho KH, Kim YS. Comparison of Efficacy and Safety between Chunghyul-dan (HH-333) and Atorvastatin (Lipitor). Korean J. Orient Int Med. 2003;24(4):837-45.
  4. Kim YM, Choi HY, Kim JD. Studies on Oral Toxicity of Eumcheonyijin-tang in Rats. J Korean Oriental Med. 2000;21(3):199-208.
  5. Wang WH, Park JH. Studies on the Safety of Artemisiae Capillaris Herba - With the Perinatal and Lactational Reproductive Toxicity. J Korean Oriental Med. 2005;26(2):32-51.
  6. Shi J, Tong Y, Shen JG, Li HX. Effectiveness and safety of herbal medicines in the treatment of irritable bowel syndrome: a systematic review. World Journal of Gastroenterology. 2008;14(3):454-62. https://doi.org/10.3748/wjg.14.454
  7. Adessi C, Soto C. Converting a peptide into a drug: strategies to improve stability and bioavailability. Curr Med Chem. 2002;9(9):963-78. https://doi.org/10.2174/0929867024606731
  8. 지형준, 이상인, 안덕균. 대한약전 및 대한약전의 한약규격주해. 서울:한국메디칼인덱스사. 1998:58-700.
  9. Asl MN, Hosseinzadeh H. Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds. Phytotherapy Research. 2008;22(6): 709-24. https://doi.org/10.1002/ptr.2362
  10. Xu GH, Kim JA, Kim SY, Ryu JC, Kim YS, Jung SH, Kim MK, Lee SH. Terpenoids and coumarins isolated from the fruits of Poncirus trifoliata. Chemical & Pharmaceutical Bulletin (Tokyo). 2008;56(6):839-42. https://doi.org/10.1248/cpb.56.839
  11. Hoult JR, Paya M. Pharmacological and biochemical actions of simple coumarins: natural products with therapeutic potential. General Pharmacology. 1996;27(4):713-22. https://doi.org/10.1016/0306-3623(95)02112-4
  12. Qian GS, Wang Q, Leung KS, Qin Y, Zhao Z, Jiang ZH. Quality assessment of Rhizoma et Radix Notopterygii by HPTLC and HPLC fingerprinting and HPLC quantitative analysis. Journal of Pharmaceutical and Biomedical Analysis. 2007;44:812-17. https://doi.org/10.1016/j.jpba.2007.03.029
  13. Corthout J, Naessens T, Apers S, Vlietinck AJ. Quantitative determination of ginsenosides from Panax ginseng roots and ginseng preparations by thin layer chromatography densitometry. Journal of Pharmaceutical and Biomedical Analysis.1999;21:187-92. https://doi.org/10.1016/S0731-7085(99)00109-0
  14. Jang IS. A Review on the Report about Druginduced Hepatitis published by the National Institute of Toxicological Research. J Korean Oriental Med. 2004;25(3):78-89.
  15. Ko SG. Safety and Efficacy of Korean Medicine and Therapeutics. Korean J Orient Int Med. 2003;24(3):531-42.
  16. Martin WM, Long HW. Complementary and alternative medicine: opportunities and challenges. J Health Care Finance. 2007;34(2):89-104.
  17. Coulter ID. Evidence based complementary and alternative medicine: promises and problems. Forsch Komplementmed. 2007;14(2):102-8. https://doi.org/10.1159/000101054
  18. Kim YH, Son CH, Moon OR, Kim SY, Lim S. A Study of Ways of Paying Insurance Benefits and the Range of Insurance Coverage of Health Insurance for Packed Herbal Medicine. J Korean Oriental Med. 2007;28(3):244-60.
  19. Hagan RL. High-performance liquid chromatography for small-scale studies of drug stability. Am J Hosp Pharm. 1994;51(17):2162-75.
  20. Kim JJ, Cho SO, Lee HY, Ha HK, Oh JH, Yang IC, Choi HS, Lee JH. Varied Flavonoid Contents of Citri Unshii Pericarpium Extracted from Several Traditional Herbal Prescriptions. J Korean Oriental Med. 2005;26(2):195-200.