Journal of Oral Medicine and Pain

Korean Academy of Orofacial Pain and Oral Medicine (대한안면통증구강내과학회)
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The Effect of S. thermophilus Isolated from Saliva Treated with Phytoncide on P. gingivalis

피톤치드 처리 후 구강 내 잔존 S. thermophilus의 P. gingivalis에 대한 효과

  • Jung, Sung-Hee (Department of Oral Medicine, School of Dentistry, Kyung Hee University) ;
  • Auh, Q-Schick (Department of Oral Medicine, School of Dentistry, Kyung Hee University) ;
  • Chun, Yang-Hyun (Department of Oral Medicine, School of Dentistry, Kyung Hee University) ;
  • Hong, Jung-Pyo (Department of Oral Medicine, School of Dentistry, Kyung Hee University)
  • 정성희 (경희대학교 치의학전문대학원 구강내과학교실) ;
  • 어규식 (경희대학교 치의학전문대학원 구강내과학교실) ;
  • 전양현 (경희대학교 치의학전문대학원 구강내과학교실) ;
  • 홍정표 (경희대학교 치의학전문대학원 구강내과학교실)
  • Published : 2009.03.30
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Abstract

The antibacterial effect of phytoncide on Porphyromonas gingivalis, which is the main causative agent of periodontal disease and halitosis, has been reported. However, little is known about its effect on normal oral microflora. The present study was performed to observe the effect of phytoncide on oral normal microflora and the inhibitory effect of surviving resident oral bacteria on P. gingivalis. In this study, saliva from each of 20 healthy subjects was treated with 1% phytoncide from Japanese Hinoki (Chamaecyparis obtusa Sieb. et Zucc.). Surviving salivary bacteria were isolated on blood agar plates and identified by 16S rDNA sequencing. In order to select inhibitory isolates against P. gingivalis, the isolates from the phytoncide-treated saliva were cultured with P. gingivalis. The results were as follows: 1. In general, the number of bacteria in saliva from periodontally healthy subjects was decreased when the saliva was treated with 1% phytoncide. 2. The majority of the salivary bacteria surviving the treatment of phytoncide were S. thermophilus (53%). 3. Most of the surviving salivary bacteria (72.5%) inhibit the growth of P. gingivalis A7A1-28 and P. gingivalis W83 on blood agar plates. 4. Among the surviving S. thermophilus, 85.8% of them were observed to inhibit P. gingivalis strains and 75.8% of the surviving S. sanguinis were inhibitory. Taken together, oral resident bacteria surviving phytoncide, which has been shown to inhibit P. gingivalis, may exert an additional inhibitory activity against the periodontopathic bacterium. Therefore, phytoncide can be used for preventing and ceasing the progress of periodontal disease and halitosis, and thus is expect to promote oral health.

치주질환과 구취를 유발시키는 중요한 원인균인 P. gingivalis에 대한 피톤치드의 항균효과와 항균작용은 이미 연구되어 있으나, 정상인의 구강상주균에 대한 연구는 아직 희귀한 편이다. 이에 본 연구에서는 건강한 정상인의 타액에 편백 피톤치드를 첨가하였을 때 사멸되지 않고 생존하는 타액세균을 분리하여 구강 유해균과 함께 배양한 후 구강 유해균에 대한 생존 타액세균의 억제효과를 파악함으로써 향후 프로바이오틱으로 작용할 수 있는 구강상주균의 균종을 동정하여 다음과 같은 결론을 얻었다. 1. 정상인의 전타액에 1% 피톤치드를 적용하였을 때 잔존 생균수는 감소하는 경향을 보였다. 2. 피톤치드 적용 후 생존한 주 세균종은 S. thermophilus (53%)로 나타났다. 3. 피톤치드 적용 후 생존한 균을 P. gingivalis A7A1-28과 P. gingivalis W83에 교차배양한 결과 생존균의 대부분(72.5%) 이 P. gingivalis A7A1-28과 P. gingivalis W83의 성장을 억제하였다. 4. 생존 S. thermophilus의 85.8%, S. sanguinis는 75.8%가 P. gingivalis 를 억제하는 것으로 나타났다. 이상의 결과로 미루어, P. gingivalis 등 구강 내 유해균을 직접 억제할 수 있는 것으로 알려진 피톤치드로 처리할 경우 피톤치드에 생존하는 구강상주균이 P. gingivalis에 대해 부가적으로 억제작용을 할 수 있기 때문에 피톤치드의 사용은 치주질환을 예방하고, 그 결과 치주질환 및 구취환자의 구강 환경을 크게 개선할 수 있을 것으로 생각된다.

Keywords

References

  1. Papapanou PN. Population studies of microbial ecology in periodontal health and disease. Ann Periodontol 2002;7:54-61 https://doi.org/10.1902/annals.2002.7.1.54
  2. 신금백. Peroxidase System 제제를 이용한 구강건조증의 치료. 타액과 타액선 토론회 1995; 55-58
  3. Welsh C. Complementary therapies in hospice care: touch with oils-a pertinent part of holistic care. Am J hospice Palliat Care 1997;Jan/Fab:42-44 https://doi.org/10.1177/104990919701400114
  4. Lis-Balchin M. Essential oils and aromatherapy: their modern role in healing. J R Soc Health 1977;117:324-329
  5. Muller CH. Allelopathy as a factor in ecological process. Vegetation 1969;18:348-357 https://doi.org/10.1007/BF00332847
  6. Gocho S. Antibacterial action of aroma compounds in vapor state. Int J Antimicrob Agents 1991;19:329-334
  7. Gocho S. The factors affecting antibacterial action of FDA vapor. Int J Antimicrob Agents 1991;19:389-393 https://doi.org/10.1016/S0924-8579(02)00012-2
  8. 谷田員光克, 大平辰朗. 바이오아스 變換計劃硏究報告. 農林水産技術會義 1990;24:36
  9. Li Q, Nakadai A, Matsushima H, Miyazaki Y, Krensky AM, Kawada T, Morimoto K. Phytoncides (wood essential oils) induce human natural killer cell activity. Immunopharmacol Immunotoxicol 2006;28:319-333 https://doi.org/10.1080/08923970600809439
  10. 강하영, 오종환. 침엽수 침엽 정유의 방향성 이용적성. 임업연보 1994;49:177-185
  11. 강하영, 이성숙, 최인규. 침엽수 수엽 정유의 항균성에 관한 연구. 한국임산에너지학회지 1993;13:71-77
  12. Whittaker RH, Feeny PP. Allelochemics, chemical interactions between species. Science 1971;171:757-770 https://doi.org/10.1126/science.171.3973.757
  13. Rice EL. Allelopathy. 2nd ed., USA, 1984, Acamedic Press Inc., pp. 79-110
  14. Haagen-Smit AJ. The essencial oils. Vol.1, USA, 1960, Van Nostrand company Inc., pp. 77-83
  15. 高橋信孝, 丸茂晋吾, 大岳 望 共著. 生理活性天然物化學. 第2版, 日本, 1981, 東京大學出版會, pp. 99-116
  16. Rudman P. The causes of natural durability in timber(9), the antifungal activity of heartwood extractives in wood substrate. Holzforschung 1962;16:74-77
  17. Rudman P. The causes of natural durability in timber(11), some tests on fungi toxisity of wood extractives and related compounds. Holzforschung 1963;17:54-57 https://doi.org/10.1515/hfsg.1963.17.2.54
  18. 강하영. 수목 추출 성분의 생화학적 역할. 목재공학 1994;22:5-11
  19. Takarada K, Kimizuka R, Takahashi N, Honma K, Okuda K, Kato T. A comparison of the antibacterial efficacies of essential oils against oral pathogens. Oral Microbiol Immunol 2004;19:61-64 https://doi.org/10.1046/j.0902-0055.2003.00111.x
  20. Schoenknecht FD, Sabath LD, Thornsberry C. Susceptibility tests: special tests. In Lennette EH, Balows A, Hausler WJ Jr, Shadomy HJ. Manual of Clinical Microbiology. 4th ed., Washington DC, 1985, American Society for Microbiolgy, pp. 1000-1008
  21. Alviano WS, Mendonca-Filho RR, Alviano DS et al. Antimicrobial activity of Croton cajucara Benth linalool-rich essential oil on artificial biofilms and planktonic microorganisms. Oral Microbiol Immunol 2005;20:101-105 https://doi.org/10.1111/j.1399-302X.2004.00201.x
  22. Cha JD, Jeong MR, Jeong SI et al. Chemical composition and antimicrobial activity of the essential oils of Artemisia scoparia and A. capillaris. Planta Med 2005;71:186-190 https://doi.org/10.1055/s-2005-837790
  23. Cha JD, Jeong MR, Choi HJ et al. Chemical composition and antimicrobial activity of the essential oil of Artemisia lavandulaefolia. Planta Med 2005;71:575-577 https://doi.org/10.1055/s-2005-864164
  24. Chung JY, Choo JH, Lee MH, Hwang JK. Anticariogenic activity of macelignan isolated from Myristica fragrans (nutmeg) against Streptococcus mutans. Phytomedicine 2006;13:261-266 https://doi.org/10.1016/j.phymed.2004.04.007
  25. Ebersole JL, Kesavalu L, Schneider SL, Machen RL, Holt SC. Comparative virulence of periodontopathogens in a mouse abscess model. Oral Dis 1995;1:115-128 https://doi.org/10.1111/j.1601-0825.1995.tb00174.x
  26. Neiders ME, Chen PB, Suido H, Reynolds HS, Zambon JJ, Shlossman M, Genco RJ. Heterogeneity of virulence among strains of Bacteroides gingivalis. J Periodontal Res 1989;24:192-198 https://doi.org/10.1111/j.1600-0765.1989.tb02005.x
  27. Golbang N, Burnie JP, Klapper PE, Bostock A, Williamson P. Sensitive and universal method for microbial DNA extraction from blood products. J Clin Pathol 1996;49:861-863 https://doi.org/10.1136/jcp.49.10.861
  28. 김각균. 구강질환에 대한 타액의 면역기능. 타액과 타액선 토론회 1995;38-41
  29. Rosan B, Lamont RJ. Dental plaque formation. Microbes Infect 2000;2:1599-1607 https://doi.org/10.1016/S1286-4579(00)01316-2
  30. Minah GE, Loesche WJ. Sucrose metabolism by prominent members of the flora isolated from cariogenic and non-cariogenic dental plaques. Infect Immun 1977;17:55-61
  31. Walker GJ, Brown RA, Taylor C. Activity of Streptococcus mutans alpha-D-glucosyltransferases released under various growth conditions. J Dent Res 1984;63:397-400 https://doi.org/10.1177/00220345840630030801
  32. Hamada S, Slade HD. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev 1980;44:331-384
  33. Whiley RA, Beighton D. Current classification of the oral streptococci. Oral Microbiol Immunol 1998;13:195-216 https://doi.org/10.1111/j.1399-302X.1998.tb00698.x
  34. Loesche WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev 1986;50:353-380
  35. Carlsson P, Gandour IA, Olsson B, Rickardsson B, Abbas K. High prevalence of mutans streptococci in a population with extremely low prevalence of dental caries. Oral Microbiol Immunol 1987;2:121-124 https://doi.org/10.1111/j.1399-302X.1987.tb00274.x
  36. Henderson B, Nair SP, Ward JM, Wilson M. Molecular pathogenicity of the oral opportunistic pathogen Actinobacillus actinomycetemcomitans. Annu Rev Microbiol 2003;57:29-55 https://doi.org/10.1146/annurev.micro.57.030502.090908
  37. Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr. Microbial complexes in subgingival plaque. J Clin Periodontol 1998;25:134-144 https://doi.org/10.1111/j.1600-051X.1998.tb02419.x
  38. Kornman KS, Loesche WJ.Effects of estradiol and progesterone on Bacteroides melaninogenicus and Bacteroides gingivalis. Infect Immun 1982;35:256-263
  39. Mombelli A, Rutar A, Lang NP. Correlation of the periodontal status 6 years after puberty with clinical and microbiological conditions during puberty. J Clin Periodontol 1995;22:300-5 https://doi.org/10.1111/j.1600-051X.1995.tb00152.x
  40. Chu L, Bramanti TE, Ebersole JL, Holt SC. Hemolytic activity in the periodontopathogen Porphyromonas gingivalis: kinetics of enzyme release and localization. Infect Immun 1991;59:1932-1940
  41. Grenier D. Characteristics of hemolytic and hemagglutinating activities of Treponema denticola. Oral Microbiol Immunol 1991;6:246-249 https://doi.org/10.1111/j.1399-302X.1991.tb00485.x
  42. Travis J, Pike R, Imamura T, Potempa J. Porphyromonas gingivalis proteinases as virulence factors in the development of periodontitis. J Periodontal Res 1997;32:120-125 https://doi.org/10.1111/j.1600-0765.1997.tb01392.x
  43. Tanner A C, Strzempko M N, Belsky C A, McKinley G A. API ZYM and API An-Ident reactions of fastidious oral gram-negative species. J Clin Microbiol 1985;22:333-335
  44. Fenno JC, Lee SY, Bayer CH, Ning Y. The opdB locus encodes the trypsin-like peptidase activity of Treponema denticola. Infect Immun 2001;69:6193-6200 https://doi.org/10.1128/IAI.69.10.6193-6200.2001
  45. Kesavalu L, Holt SC, Ebersole JL. Trypsin-like protease activity of Porphyromonas gingivalis as a potential virulence factor in a murine lesion model. Microb Pathog 1996;20:1-10 https://doi.org/10.1006/mpat.1996.0001
  46. DeCarlo AA, Grenett HE, Harber GJ, Windsor LJ, Bodden MK, Birkedal-Hansen B, Birkedal-Hansen H. Induction of matrix metalloproteinases and a collagen-degrading phenotype in fibroblasts and epithelial cells by secreted Porphyromonas gingivalis proteinase. J Periodontal Res 1998;33:408-420 https://doi.org/10.1111/j.1600-0765.1998.tb02337.x
  47. Potempa J, Banbula A, Travis J. Role of bacterial proteinases in matrix destruction and modulation of host responses. Periodontol 2000;24:153-192 https://doi.org/10.1034/j.1600-0757.2000.2240108.x
  48. Cox SD, Mann CM, Marham JL. Interactions between components of the essential oil of Melaleuca alternifolia. J Appl Microbiol 2000;88:170-175 https://doi.org/10.1046/j.1365-2672.2000.00943.x
  49. 이종한. 피부감염모낭충에 대한 피톤시드의 역할. 중앙대학교 학위논문 2005:19-21
  50. Lovett JV, Ryuntyu MY Liu DL. Allelopathy Chemical communication and plant defense. J Chem Ecol 1989;15:1193-1202 https://doi.org/10.1007/BF01014822
  51. Patrick ZA. Allelopathy mechanism and their exploitation for biological control. Anandian J Plant Pathol 1986;8:225-228 https://doi.org/10.1080/07060668609501831
  52. Wink M. Plant breeding, importance of plant secondary metabolites for protection against pathogens and herbivores. Ther Appl Gen 1988;75:225-233 https://doi.org/10.1007/BF00303957
  53. 강하영. 피톤치드의 비밀. 역사넷 2003:15-20
  54. 김선규, 신미경, 어규식, 이진용, 홍정표, 전양현. P. gingivalis에 대한 피톤치드의 항균효과. 대한구강내과학회지 2007;32:137-150
  55. 박재봉, 어규식, 전양현, 이진용, 홍정표. 피톤치드의 입냄새 제거효과. 대한구강내과학회지 2007;32:151-156
  56. Nakano Y, Yoshimura M, Koga T. Methyl mercaptan production by periodontal bacteria. Int Dent J 2002;52(Suppl 3):217-220 https://doi.org/10.1002/j.1875-595X.2002.tb00928.x
  57. Guarner F, Perdigon G, Corthier G, Salminen S, Koletzko B, Morelli L. Should yoghurt cultures be considered probiotic? Br J Nutr 2005;93:783-786 https://doi.org/10.1079/BJN20051428
  58. Meurman JH. Probiotics: do they have a role in oral medicine and dentistry? Eur J Oral Sci 2005;113:188–196 https://doi.org/10.1111/j.1600-0722.2005.00191.x
  59. Stackebransdt E, Liesack W. Nucleic acids and classification. In Handbook of New Bacterial Systematics (edited by Goodfellow M, O'Donnell AG), Academic Press. 1993:151-194
  60. Kononen E, Jousimies-Somer H, Bryk A, Kilp T, Kilian M. Establishment of streptococci in the upper respiratory tract: longitudinal changes in the mouth and nasopharynx up to 2 years of age. J Med Microbiol 2002;51:723-730 https://doi.org/10.1099/0022-1317-51-9-723
  61. Dagan R. Treatment of acute otitis media - challenges in the era of antibiotic resistance. Vaccine 2000;19(Suppl 1):S9–S16 https://doi.org/10.1016/S0264-410X(00)00272-3
  62. Kawamura Y, Hou XG, Sultana F, Miura H, Ezaki T. Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus. Int J Syst Bacteriol 1995;45:406-408 https://doi.org/10.1099/00207713-45-2-406
  63. Amano A, Kuboniwa M, Nakagawa I, Akiyama S, Morisaki I, Hamada S. Prevalence of specific genotypes of Porphyromonas gingivalis fimA and periodontal health status. J Dent Res 2000;79:1664-1668 https://doi.org/10.1177/00220345000790090501
  64. Ivanova I, Miteva V, Stefanova TS et al. Characterization of a bacteriocin produced by Streptococcus thermophilus 81. Int J Food Microbiol 1998;42:147-158 https://doi.org/10.1016/S0168-1605(98)00067-1
  65. Kabuki T, Uenishi H, Watanabe M, Seto Y, Nakajima H. Characterization of a bacteriocin, Thermophilin 1277, produced by Streptococcus thermophilus SBT1277. J Appl Microbiol 2007;102:971-980
  66. Kim YJ, Seol YJ, Choi BK, Le SH. Preventive effect of the functional fermented milk contained probiotic cultures and Curcuma xanthorrhiza extract on oral disease. 제15회 국제학술심포지엄 보고서(유산균과 건강-유산균과 구강건강) 2007;82-95
  67. Saavedra JM, Abi-Hanna A, Moore N, Yolken RH. Long-term consumption of infant formulas containing live probiotic bacteria: tolerance and safety. Am J Clin Nutr 2004;79:261-267 https://doi.org/10.1093/ajcn/79.2.261