Animal cells and systems

  • 제13권1호
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  • Pages.17-23
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  • 2009
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  • 1976-8354(pISSN)
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  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지

Annexin I Stimulates Insulin Secretion through Regulation of Cytoskeleton and PKC Activity

  • Kang, Na-Na (Department of Biological Sciences and Institute for Basic Sciences, Sungkyunkwan University) ;
  • Won, Jong-Hak (Department of Pharmacology and Physiology, University of Rochester) ;
  • Park, Young-Min (Department of Biological Sciences and Institute for Basic Sciences, Sungkyunkwan University)
  • 발행 : 2009.03.31
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초록

In previous studies, we found that Annexin I (Anx I) was co-secreted with insulin in response to glucose, and that extracellular Anx I stimulated the release of insulin via the Anx I binding site in rat pancreatic islets and the &-cell line. However, the role that Anx I plays in the insulin secretion was not established. Therefore, in this study, we evaluated the insulin secretion pattern in response to Anx I and the involvement of the cytoskeleton or PKC in Anx Istimulated insulin secretion in MIN6N8a cells. The peak time of insulin secretion in response to Anx I treatment corresponded with the second phase insulin secretion by glucose in the perifused pseudoislets. In addition, Anx I-stimulated insulin secretion was not affect by readily releasable pool depletion. Taken together, these findings indicate that Anx I treatment was associated with movement of the reserve pool of insulin. Furthermore, Anx I-stimulated insulin secretion was attenuated by treatment with a microfilament inhibitor, cytochalasin B, as well as by PKC down regulation. These results indicate that Anx I may be a regulator of second phase insulin secretion.

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