Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Bone Cell Response to Neurotransmitters and Mechanical Loading

신경전달물질 및 물리적 자극에 대한 뼈 세포의 반응

  • Kwag, J.H. (Department of Biomedical Engineering, Yonsei University) ;
  • Kim, B.G. (Department of Biomedical Engineering, Yonsei University) ;
  • Kim, K.H. (Department of Biomedical Engineering, Yonsei University) ;
  • Kim, C.H. (Department of Biomedical Engineering, Yonsei University)
  • 곽지현 (연세대학교 보건과학대학 의공학과) ;
  • 김병관 (연세대학교 보건과학대학 의공학과) ;
  • 김경환 (연세대학교 보건과학대학 의공학과) ;
  • 김지현 (연세대학교 보건과학대학 의공학과)
  • Published : 2009.02.28
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Abstract

Bone remodeling is a continuous process of skeletal renewal during which bone formation is tightly coupled to bone resorption. Mechanical loading is an important regulator of bone formation and resorption. In recent studies, neurotransmitters such as vasoactive intestinal peptide (VIP) were found to be present inside bone tissue and have been suggested to potentially regulate bone remodeling. In this study, our objective was to use a pre-established in vitro oscillatory fluid flow-induced shear stress mechanical loading system to quantify the effect of VIP on bone resorptive activity and investigate its combined effect with mechanical loading. VIP decreased osteoclastogenesis significantly decreased RANKL/OPG mRNA ration by approximately 90%. Combined VIP and mechanical loading further decreased RANKL/OPG ratio to approximately 95%. These results suggest that VIP present in bone tissue may synergistically act with mechanical loading to regulate bone remodeling via suppression of bone resorptive activities.

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References

  1. M.M. Cohen, Jr., 'The new bone biology: pathologic, molecular, and clinical correlates,' Am J Med Genet A, vol.140, pp.2646-2706, 2006
  2. C.T. Rubin and L.E. Lanyon, 'Regulation of bone formation by applied dynamic loads,' J Bone Joint Surg Am, vol.66, pp.397-402, 1984 https://doi.org/10.2106/00004623-198466030-00012
  3. I. Owan, D.B. Burr, C.H. Turner, J. Qiu, Y. Tu, J.E. Onyia, and R.L. Duncan, 'Mechanotransduction in bone: osteoblasts are more responsive to fluid forces than mechanical strain,' Am J Physiol, vol.273, pp.C810-815, 1997 https://doi.org/10.1152/ajpcell.1997.273.3.C810
  4. C.H. Kim, L. You, C.E. Yellowley, and C.R. Jacobs, 'Oscillatory fluid flow-induced shear stress decreases osteoclastogenesis through RANKL and OPG signaling,' Bone, vol.39, pp.1043-1047, 2006 https://doi.org/10.1016/j.bone.2006.05.017
  5. S. Harada and G.A. Rodan, 'Control of osteoblast function and regulation of bone mass,' Nature, vol.423, pp.349-355, 2003 https://doi.org/10.1038/nature01660
  6. M. Asagiri and H. Takayanagi, 'The molecular understanding of osteoclast differentiation,' Bone, vol.40, pp.251-264, 2007 https://doi.org/10.1016/j.bone.2006.09.023
  7. U.H. Lerner, 'Neuropeptidergic regulation of bone resorption and bone formation,' J Musculoskelet Neuronal Interact, vol.2, pp.440-447, 2002
  8. F. Elefteriou, 'Neuronal signaling and the regulation of bone remodeling,' Cell Mol Life Sci, vol.62, pp.2339-2349, 2005 https://doi.org/10.1007/s00018-005-5175-3
  9. P. Lundberg and U.H. Lerner, 'Expression and regulatory role of receptors for vasoactive intestinal peptide in bone cells,' Microsc Res Tech, vol.58, pp.98-103, 2002 https://doi.org/10.1002/jemt.10124
  10. P. Lundberg, I. Bostrom, H. Mukohyama, A. Bjurholm, K. Smans, and U.H. Lerner, 'Neuro-hormonal control of bone metabolism: vasoactive intestinal peptide stimulates alkaline phosphatase activity and mRNA expression in mouse calvarial osteoblasts as well as calcium accumulation mineralized bone nodules,' Regul Pept, vol.85, pp.47-58, 1999 https://doi.org/10.1016/S0167-0115(99)00069-5
  11. H. Takayanagi, 'Inflammatory bone destruction and osteoimmunology' J Periodontal Res, vol.40, pp.287-293, 2005 https://doi.org/10.1111/j.1600-0765.2005.00814.x
  12. W.J. Boyle, W.S. Simonet, and D.L. Lacey, 'Osteoclast differentiation and activation,' Nature, vol.423, pp.337-342, 2003 https://doi.org/10.1038/nature01658
  13. N.N. Batra, Y.J. Li, C.E. Yellowley, L. You, A.M. Malone, C.H. Kim, and C.R. Jacobs, 'Effects of short-term recovery periods on fluid-induced signaling in osteoblastic cells,' J Biomech, vol.38, pp.1909-1917, 2005 https://doi.org/10.1016/j.jbiomech.2004.08.009
  14. T. Suda, N. Takahashi, N. Udagawa, E. Jimi, M.T. Gillespie, and T.J. Martin, 'Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families,' Endocr Rev, vol.20, pp.345-357, 1999 https://doi.org/10.1210/er.20.3.345
  15. E.L. Hohmann, L. Levine, and A.H. Tashjian, Jr., 'Vasoactive intestinal peptide stimulates bone resorption via a cyclic adenosine 3',5'-monophosphate-dependent mechanism,' Endocrinology, vol.112, pp.1233-1239, 1983 https://doi.org/10.1210/endo-112-4-1233