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The Antimicrobial Activity of Gwankeibujalijung-tang Against Streptococcus pneumoniae 59 Isolated from the Mouth of a Common Cold Patient

감기환자의 구강에서 분리된 Streptoccous pneumoniae 59에 대한 관계부자이중탕(官桂附子理中湯)의 효과

  • Lee, Sang-Yun (Department of Oriental Herbology, Daegu Haany University) ;
  • Seo, Bu-Il (Department of Oriental Herbology, Daegu Haany University) ;
  • Park, Ji-Ha (Department of Oriental Herbology, Daegu Haany University) ;
  • Roh, Seong-Soo (Department of Oriental Herbology, Daegu Haany University) ;
  • Lee, Eun-Sook (Department of Herbal Skin Care, Daegu Haany University) ;
  • Kim, Yong-Hyun (Department of Language Education, Daegu Haany University)
  • 이상윤 (대구한의대학교 한의학과 본초학교실) ;
  • 서부일 (대구한의대학교 한의학과 본초학교실) ;
  • 박지하 (대구한의대학교 한의학과 본초학교실) ;
  • 노성수 (대구한의대학교 한의학과 본초학교실) ;
  • 이은숙 (대구한의대학교 한방피부미용학과) ;
  • 김용현 (대구한의대학교 어학교육원)
  • Published : 2009.03.30

Abstract

Objectives:I want to examine the antimicrobial activity of Gwankeibujalijung-tang against Streptococcus pneumoniae 59 isolated from the mouth of a common cold patient. Methods: Antimicrobial activity was assayed through the hot water extract from Gwankeibujalijung-tang against Streptococcus pneumoniae 59 isolated from the mouth of a common cold patient. Results: The size of inhibition zone of Gwankeibujalijung-tang extract was 9.83 $\pm$ 0.11 mm. The optimal pH and temperature for the growth of isolated Streptococcus pneumoniae 59 were 7.0 and 37$^{\circ}C$, respectively. The minimum inhibitory concentration of Gwankeibujalijung-tang extract was 12 $\mu\ell$ and the antimicrobial activity of Gwankeibujalijung-tang extract was not destroyed by the heat (121$^{\circ}C$ for 15 min) and not affected by pH. Conclusions: Reviewing this experimental result, it appeared that Gwankeibujalijung-tang had efficacy against Streptococcus pneumoniae 59 isolated from the mouth of a common cold patient.

Keywords

References

  1. Watson, DA, Musher, DM, Jacobson JW and Verhoef J. A brief history of the pneumococcus in biomedical research: a panoply of scientific discovery. Clin Infect Dis. 1993. Nov ; 17(5) : 913-24. https://doi.org/10.1093/clinids/17.5.913
  2. Dagan R, Isaachson M, Lang R, Karpuch J, Block C and Amir J. For the Israeli Pediatric Bacteremia and Meningitis Group. Epidemiology of pediatric meningitis caused by Haemophilus influenzae type b, Streptococcus pneumoniae, and Neisseria meningitidis in Israel: a 3-year nationwide prospective study. J Infect Dis. 1994 ; 169 : 912-6. https://doi.org/10.1093/infdis/169.4.912
  3. Gray BM, Converse GM and Dillon HC Jr. Serotypes of Streptococcus pneumoniae causing disease. J Infect Dis. 1979 ; 140 : 979-83. https://doi.org/10.1093/infdis/140.6.979
  4. Musher DM. Infections caused by Streptococcus pneumoniae: clinical spectrum, pathogenesis, immunity, and treatment. Clin Infect Dis. 1992 ; 14 : 801-9. https://doi.org/10.1093/clinids/14.4.801
  5. 이제마 원저. 동의학연구소 역. 原文對譯 東醫壽世保元. 서울:여강출판사. 2002:96-7, 102-3, 106-9, 148.
  6. Smibert RM and Krieg NR. General characterization, In Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR and Phillips GB (ed.). Manual of methods for general bacteriology. Washington DC : American Society for Microbiology. 1981 ; 409-43.
  7. Facklam R and Elliott JA. Identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev. 1995 ; 8 : 479-95.
  8. Burdash NM and West ME. Identification of Streptococcus pneumoniae by the Phadebact coagglutination test. J Clin Microbiol. 1982 March ; 15(3) : 391-4.
  9. Sottile MI. and Rytel MW. Application of counterimmunoelectrophoresis in the identification of Streptococcus pneumoniae in clinical isolates. J Clin Microbiol. 1975 Sep ; 2(3) : 173-7.
  10. Browne K, Miegel J and Stottmeier KD. Detection of pneumococci in blood cultures by latex agglutination. J Clin Microbiol. 1984 May ; 19(5) : 649–50.
  11. Zaika LL. Spices and herbs: their antibacterial activity and its determination. J Food Saf. 1988 ; 23 : 97-118.
  12. 오덕환, 함흥시, 박부길, 안철, 유진영. 식품부패 및 병원미생물에 대한 천연약용식물 추출물의 항균효과. 한국식품과학회지. 1998 ; 30(4) : 957-63.
  13. 김진상. 소음인 升陽益氣附子湯과 官桂附子理中湯이 陽虛證에 미치는 影響에 관한 실험적 연구. 사상의학회지 1989 ; 1(1) : 87-112.
  14. Bluestone CD, Stephenson JS and Martin LM. Ten-year review of otitis media pathogens. Pediatr. Infect Dis J. 1992 ; 11(Suppl. 8) : 7-11. https://doi.org/10.1097/00006454-199208001-00002
  15. Sorensen UBS, Blom J, Birch-Andersen A and Henrichsen J. Ultrastructural localization of capsules, cell wall polysaccharide, cell wall proteins, and F antigen in pneumococci. Infect Immun. 1988 ; 56 : 1890-1896.
  16. Mundy L S, Janoff EN, Schwebke KE, Shanholtzer CJ and Willard KE. Ambiguity in the identification of Streptococcus pneumoniae. Optochin, bile solubility, quellung, and the AccuProbe DNA probe tests. Am J Clin Pathol. 1998 ; 109 : 55-61. https://doi.org/10.1093/ajcp/109.1.55
  17. Neeleman C, S Geelen, P Aerts, M Van Tilburg, D Watson, J Verhoef, A Fleer and H Van Dijk. Virulence of Streptococcus pneumoniae is associated with a regulator factor H. In Program and abstracts of the 33rd Interscience Conference on Antimicrobial Agents and Chemotherapy. American Society for Microbiology, Washington, D.C. 1993 : 219. abstr. 548.
  18. Johnston RB Jr. Pathogenesis of pneumococcal pneumonia. Rev. Infect Dis. 1991 ; 13(Suppl. 6) : S509-S17.
  19. Duane PG, Rubins JB, Weisel HR and Janoff EN. Identification of hydrogen peroxide as a Streptococcus pneumoniae toxin for rat alveolar epithelial cells. Infect Immun. 1993 ; 61 : 4392-7.