Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Quantitative Determination of Diterpenoids from the Roots of Aralia cordata

  • Lee, Mi-Kyoung (College of Pharmacy, Catholic University of Daegu) ;
  • Hung, Tran Manh (College of Pharmacy, Catholic University of Daegu) ;
  • Min, Byung-Sun (College of Pharmacy, Catholic University of Daegu) ;
  • Lee, Ik-Soo (College of Pharmacy, Chungnam National University) ;
  • Na, Min-Kyun (College of Pharmacy, Yeungnam University) ;
  • Woo, Mi-Hee (College of Pharmacy, Catholic University of Daegu) ;
  • Son, Jong-Keun (College of Pharmacy, Yeungnam University) ;
  • Kim, Young-Ho (College of Pharmacy, Chungnam National University) ;
  • Choi, Jae-Sue (Faculty of Food Science and Biotechnology, Pukyoung National University) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
  • Published : 2009.03.31
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Abstract

A simple and reliable reversed phase HPLC method was developed to determine pharmacologically active compounds ent-continentalic acid, kaurenoic acid, and continentalic acid from the roots of Aralia cordata. Quantitative analysis of diterpenoids of A. cordata were performed by reverse phase C-18 column using a isocratic of acetonitrile : 0.1% trifluoroacetic acid(70:30) with UV detection at 205 nm. The correlation coefficients of each calibration curve of ent-continentalic acid, kaurenoic acid, and continentalic acid were 0.9987, 0.9983, and 0.9986, respectively. The detection limit of ent-continentalic acid, kaurenoic acid, and continentalic acid were $0.5{\sim}1.0\;{\mu}g/mL$, respectively. The contents of ent-continentalic acid (1), kaurenoic acid (2), and continentalic acid (3) of the roots of A. cordata collected from seventeen district markets in Korea were $0.09{\sim}0.75\;mg/g$, $1.09{\sim}5.43\;mg/g$, and $2.69{\sim}9.08\;mg/g$, respectively.

Keywords

References

  1. Baek, Y.H., Huh, J.E., Lee, J.D., Choi, D.Y., and Park, D,S., Effect ofAralia cordata extracts on cartilage protection and apoptosisinhibition. Biol. Pharm. Bull. 29, 1423-1430 (2006) https://doi.org/10.1248/bpb.29.1423
  2. Dang, N.H., Zhang, X.F., Zheng, M.S., Son, K.H., Chang, H.W., Kim,H.P., Bae, K.H., and Kang, S.S., Inhibitory constituents againstcyclooxygenases from Aralia cordata Thunb. Arch. Pharm. Res. 28,28-33 (2005) https://doi.org/10.1007/BF02975131
  3. Han, B.H., Han, Y.N., Han, L.A., Park, M.H., and Lee, E.O., Studies onthe anti-inflammatory activity of Aralia continentalis (I). Arch. Pharm.Res. 6, 17-23 (1983) https://doi.org/10.1007/BF02855697
  4. Ito, T., Tsukiji, K., and Odagiri, S., Constituents of the head space gas ofUdo (Aralia cordata Thunb.). Nippon Nogeikagaku Kaishi 52, 223-224 (1978) https://doi.org/10.1271/nogeikagaku1924.52.5_223
  5. Kim, J.S. and Kang, S.S., Saponins from the aerial parts of Araliacontinentalis. Nat. Prod. Sci. 4, 45-50 (1998)
  6. Kim, Y.H., Kim, H.S., Lee, S.W., Masakazu, U., and Lee J.J., Kauranederivatives from Acanthopanax koreanum. Phytochemistry 39, 449-451 (1995) https://doi.org/10.1016/0031-9422(95)99385-2
  7. Kwon, H.C. and Lee, K.R., Phytochemical constituents of Artemisiajaponica ssp. littoricola. Arch. Pharm. Res. 24, 194-197 (2001) https://doi.org/10.1007/BF02978255
  8. Lee, I.S., Jin, W.Y., Zhang, X., Hung, T.M., Song, K.S., Seong, Y.H., andBae, K.H., Cytotoxic and COX-2 inhibitory constituents from theaerial parts of Aralia cordata. Arch. Pharm. Res. 29, 548-555 (2006) https://doi.org/10.1007/BF02969263
  9. Lee, S.M., Park, J.K., and Lee, C.G., Quantitative determination oftriterpenoids from the fruits of Zizyphus jujuba. Natural ProductSciences 10, 93-95 (2004)
  10. Li, K., and Wang, H., Simultaneous determination of matrine, sophoridineand oxymetrine in Sophora flavescens Ait. by high performance liquidchromatography. Biomed. Chromatogr. 18, 178-182 (2004) https://doi.org/10.1002/bmc.308
  11. Okuyama, E., Nishimura, S., and Yamazaki, M., Analgesic principlesfrom Aralia cordata Thunb. Chem. Pharm. Bull. 39, 405-407 (1991)
  12. Park, S.Y. and Kim, J.W., Cytotoxic Polyacetylenes from Aralia cordata.Yakhak Hoeji 39, 681-688 (1995)
  13. Perry, L.M., Medicinal Plants of East and Southeast Asia: AttributedProperties and Uses; The MIT Press, Cambridge, p. 41 (1980)
  14. Sawamura, M., Kim, M.S., Shichiri, K., Tsuji, T., and Machida, K.,Volatile constituents of Japanese and Korean Udo (Aralia cordataThunb.) and Butterbur (Petasites japonicus Miq.). Research Reportsof the Kochi University 38, 49-60 (1989)
  15. Seo, C.S., Li, G., Kim, C.H., Lee, C.S., Jahng, Y., Chang H.W., and Son,J.K., Cytotoxic and DNA topoisomearses I and II inhibitoryconstituents from the roots of Aralia cordata. Arch. Pharm. Res. 30,1404-1411 (2007) https://doi.org/10.1007/BF02977364
  16. Xie, N., Zhong, S.M., Zjao, S.X., Waterman, P.G., He, C.H., and Zheng,Q.T., Diterpenes from Pseuduvaria indochinensis. J. Chin. Pharm.Univ. 20, 203-207 (1989)
  17. Xu, X.D., Zhang, S.M., Zhang, Y.M., Yang, J.S., Wang, C.L., Zhu, Z.Y.,and Chen, J.M., Determination of diterpenoic acid, triterpenoids andtritersaponins in Aralia by RP-HPLC. Acta Pharmaceutical Sinica 33,933-936 (1998)
  18. Yoshihara, K. and Hirose, Y., Terpenes from Aralia Species.Phytochemistry 12, 468 (1973) https://doi.org/10.1016/0031-9422(73)80044-5