Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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The Study on the Whitening Effects of Traditional Chinese Medicines

중의 약재로부터 피부 미백제의 탐색

  • ;
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  • 이수연 ((주)바이오랜드 생명공학연구소) ;
  • 김영희 ((주)바이오랜드 생명공학연구소) ;
  • 박선기 ((주)코스메카 중앙연구소) ;
  • 오선택 ((주)코스메카 중앙연구소) ;
  • 김기호 ((주)바이오랜드 생명공학연구소)
  • Published : 2009.12.30
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Abstract

In order to search for new whitening cosmetic ingredients from Chinese herbal extracts including Chinese herbs and complex prescriptions from TCM (Traditional Chinese Medicine), we screened about 47 TCM extracts collected from China. We tested their inhibitory effects on melanogenesis by using in vitro tyrosinase inhibition assay and B16 melanoma cells. We selected Siphonostegia chinensis and Salvianic miltiorrhiza Bunge. We isolated Danshensu ($\alpha$,3,4-trihydroxybenzenepropanoic acid sodium salt) from Salvianic miltiorrhiza Bunge extract and tested its inhibitory effect on melanin formation in B16-F10 melanoma cells. Danshensu suppressed melanin synthesis up to about 50% at a concentration of $100\;{\mu}g/mL$. Siphonostegia chinensis suppressed melanin synthesis up to about 60% at a concentration of $300\;{\mu}g/mL$. The results showed that these extracts could be used as new natural active ingredients for whitening cosmetics.

본 연구에서는 미백 화장품 원료를 개발하기 위해 중국에서 전통적으로 사용되어 오던 47종의 천연약재 또는 복합처방 중에서 멜라닌 생성 저해 효과를 지닌 원료를 찾고자 하였다. 그 중 버섯 티로시나제 활성 실험에서는 저해 효과를 보이지 않았으나, B16-F10 멜라닌 생성세포(B16-F10 melanoma cell)를 이용하여 류기노와 단삼을 선별하였으며 단삼에서 단삼소(丹蔘素, $\alpha$,3,4-trihydroxybenzenepropanoic acid sodium salt)를 분리하여 B16-F10 멜라노마 세포를 이용하여 멜라닌 생성 억제에 관한 실험을 실시하였다. 류기노와 단삼소는 농도에 따라 멜라닌 생성을 억제하였으며, 류기노는 $300\;{\mu}g/mL$의 농도에서 약 60%, 단삼소는 $100\;{\mu}g/mL$의 농도에서는 약 50%의 멜라닌 생성 저해 효과를 보였다. 따라서 연구 결과로써 얻어진 2종의 원료는 새로운 천연 미백 소재로 적용할 수 있을 것으로 기대된다.

Keywords

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