Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Immunoblotting Reactivity of Vitellogenin Antibodies against Native Vitellogenins and a Vitellogenin Protein Fragment Produced in E. coli

  • Published : 2009.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Vitellogenin (Vtg) is found in the serum of both female and male fish that have been exposed to environmental endocrine disrupters or estrogen hormones, and is used as a biomarker for such contamination. In our current study antibodies raised against the purified flatfish Vtg were tested for their reactivity on immunoblots to flatfish and carp Vtg, and also to a Vtg protein fragment produced in E. coli. Polyclonal antibodies raised against purified flatfish Vtg reacted well with Vtg in the serum of flatfish and carp induced with $17{\beta}$-estradiol, but not with the Vtg protein fragment produced in E. coli.

Keywords

References

  1. Burnette WN (1981) Western Blotting: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112, 195-203 https://doi.org/10.1016/0003-2697(81)90281-5
  2. Chen TT (1983) Identification and characterization of estrogenresponsive gene products in the liver of rainbow trout. Can J Biochem Cell Biol 61, 802-810 https://doi.org/10.1139/o83-102
  3. Colborn T and Clement C (1992) Chemically induced alterations in sexual and functional development. In The Wildlife/Human Connection, Princeton Scientific Publishing, Princeton, NJ, USA
  4. Custodia-Lora N, Novillo A, and Callard IP (2004) Effect of gonadal steroids on progesterone receptor, estrogen receptor, and vitellogenin expression in male turtles (Chrysemys picta). J Exp Zoology 301, 15-25
  5. Kim N, Park IS, and Kim DK (2006a) Optimization of quartz crystal microbalance-precipitation sensor measuring acetylcholinesterase activity. J Microbiol Biotechnol 16, 1523-1528
  6. Kim N, Park IS, and Kim WY (2006b) Detection of carp vitellogenin with piezoelectric immunosensor. J Korean Soc Appl Biol Chem 49, 254-258
  7. Kim N, Park IS, and Kim WY (2007a) Salmonella detection with a direct-binding optical grating coupler immunosensor. Sens Actuators B Chem 121, 606-615 https://doi.org/10.1016/j.snb.2006.04.094
  8. Kim N, Ryu HS, and Kim WY (2007b) Flatfish vitellogenin detection using optical waveguide lightmode spectroscopybased immunosensor. J Microbiol Biotechnol 17, 1445-1451
  9. Kolosova AY, Samsonova YV, and Egorov AM (2000) Competitive ELISA of chloramphenicol: Influence of immunoreagent structure and application of the method for the inspection of food of animal origin. Food Agric Immunol 12, 115-125 https://doi.org/10.1080/095401000404067
  10. Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227, 680-685 https://doi.org/10.1038/227680a0
  11. Moon DK, Kim N, and Kim WY (2006) Reactivity of the antibodies against purified carp vitellogenin and a synthetic vitellogenin peptide. J Korean Soc Appl Biol Chem 49, 196-201
  12. Nam YS and Choi JW (2006) Fabrication and electrical characteristics of ferredoxin self-assembled layer for biomolecular electronic device application. J Microbiol Biotechnol 16, 15-19
  13. Oh BK, Kim YK, Park KW, Lee WH, and Choi JW (2004) Surface plasmon resonance immunosensor for the detection of Salmonella typhimurium. Biosens Bioelectron 19, 1497-1504 https://doi.org/10.1016/j.bios.2003.12.009
  14. Park JS, Lim SH, Sim SJ, Chae H, Yoon HC, Yang SS, and Kim BW (2006) Enhancement of sensitivity in interferometric biosensing by using a new biolinker and prebinding antibody. J Microbiol Biotechnol 16, 1968-1976
  15. Smeets JMW, Rankouhi TR, Nichols KM, Komen H, Kaminski NE, Giesy JP, and Martin van den Berg M (1999) In vitro vitellogenin production by carp (Cyprinus carpio) hepatocytesas a screening method for determining (anti)estrogenic activity of xenobiotics. Toxico Appl Pharmacol 157, 68-76 https://doi.org/10.1006/taap.1999.8663
  16. Tyler CR, Van der Eerden B, Sunpter JP, Jobling S, and Painter G (1996) Measurement of vitellogenin, a biomarker for exposure to oestrogen, in a wide variety of cyprinids. J Comp Physiol 166, 418-426 https://doi.org/10.1007/BF02337886
  17. Tyler CR, Van Aerle R, Nilsen MV, Blackwell R, Maddix S, Nilsen BM, Berg K, Hutchinson TH, and Goksoyr A (2002) Monoclonal antibody enzyme-linked immunosorbent assay to quantify vitellogenin for studies on environmental estrogens in the rainbow trout (Oncorhynchus mykiss). Environ Toxicol Chem 21, 47-54 https://doi.org/10.1897/1551-5028(2002)021<0047:MAELIA>2.0.CO;2
  18. Wallace RA (1985) Vitellogenesis and oocyte growth in nonmammalian vertebrates. In Developmental Biology, Browder LW (ed.), pp. 127-177. Plenum Press, New York, USA

Cited by

  1. Development of Indirect-Competitive Optical Waveguide Lightmode Spectroscopy-based Immunosensor for Measuring Sulfamethazine vol.12, pp.2, 2018, https://doi.org/10.1007/s13206-017-2205-9