Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Inhibitory Effect of PME88 MelonSOD on the Ultraviolet-Induced Photo-aging

PME88 멜론SOD의 자외선으로 인한 피부 광노화 억제 효과

  • Published : 2009.04.30
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Abstract

PME88 (gliadin-combined) melon superoxide dismutase (SOD) is known to promote the production of the body‘s own natural antioxidants including superoxide dismutase, catalase and glutathione peroxidase. In this study, we investigated the inhibitory effects of PME88 melonSOD on the ultraviolet-induced photo-aging by the evolution of minimal erythemal dose (MED), erythema quotation and spectrocolorimetric measurements of erythema. The analysis of the evolution of the MED showed a significant increase 28 days after the daily taken of the PME88 melonSOD. The analysis of the erythema quotation showed that on D29, for the dose 1.25 MED, erythema intensity is significantly higher for placebo group than for PME88 melonSOD group. At doses 0.64 MED$_{D14}$, 0.80 MED$_{D14}$ and 1 MED$_{D14}$ the value of parameter $a^*$ (the most sensitive to the colour changes bound to the variations of blood flow. It permits to assess the evolution of erythema) is significantly higher for placebo group. No significant difference has been observed between groups (PME88 melonSOD and placebo) on the evolution of the number and consistency of feces after 4 weeks of treatment. No intolerance has been observed during the 4 weeks of treatment. These results mean that PME88 melonSOD as a dietary supplement could be useful to attenuate ultraviolet-induced skin photo-aging.

PME88 멜론(글리아딘과 결합된 식물 추출물) superoxide dismutase(SOD)의 섭취는 체내에 존재하는 superoxide dismutase, catalase 및 glutathione peroxidase를 포함한 항산화 효소의 활성 및 총량을 증가시키는 것으로 이전 연구에서 확인되었다. 본 연구에서 minimal erythema dose (MED) 값의 변화, 홍반점수의 변화, 분광비색계 측정값을 분석함으로써 자외선으로 인한 피부 광노화 억제 효과를 알아보았다. MED 값의 변화 분석에서는 PME88 멜론SOD 섭취 군에서는 시험 물질을 28일 동안 섭취한 후 MED가 유의하게 증가하였으며, 14일과 28일 사이에 그 유의성은 더욱 증가하였다. 홍반의 심한 정도(홍반점수) 분석에서 28일 동안 섭취 후 위약 군이 PME88 멜론SOD 투여군보다 1.25 MED의 자외선을 조사하였을 경우 홍반이 유의하게 나타났다. 또한 분광비색계 측정값 분석에서 0.64 MED$_{D14}$, 0.80 MED$_{D14}$, 1.0 MED$_{D14}$에서 $a^*$변수(색변화에 가장 민감한 변수, 홍반의 색조 변화를 반영)가 위약 군에서 PME88 멜론 SOD 투여군보다 유의하게 높았다. 4주간의 시험기간 동안 대변에 대한 횟수나 성향의 이상 징후 및 거부반응은 없었다. 이상의 결과들을 종합하면 PME88 멜론SOD의 섭취는 안전하게 UV로 인한 피부의 광노화를 억제하는데 유용하다는 사실을 확인하였다.

Keywords

References

  1. Niwa Y, Kasama T, Kawai S, Komura J, Sakane T, Kanoh T, Miyachi Y. 1988. The effect of aging on cutaneous lipid peroxide levels and superoxide dismutase activity in guinea pigs and patients with burns. Life Sci 42: 351-356 https://doi.org/10.1016/0024-3205(88)90072-0
  2. Freeman BA, Crapo JD. 1982. Biology of disease: free radicals and tissue injury. Lab Invest 47: 412-426
  3. McCord JM, Fridovich I. 1969. Superoxide dismutase: an enzymic function forerythrocuprein (hemocuprein). J Biol Chem 244: 6049-6055
  4. Duh PD, Tu YY, Yen GC. 1999. Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat). Lebensm Wiss Technol 32: 269-277 https://doi.org/10.1006/fstl.1999.0548
  5. Osborn-Barnes HT, Akoh CC. 2003. Effect of $\alpha-tocopherol, \beta$-carotene, and isoflavones on lipid oxidation of structured lipid-based emulsions. J Agric Food Chem 51: 6856-6860 https://doi.org/10.1021/jf026212s
  6. Yen GC, Hsieh CL. 1998. Antioxidant activity of extracts from Du-zhong (Eucommia ulmoides) toward various lipid peroxidation models in vitro. J Agric Food Chem 46: 3431-3436 https://doi.org/10.1021/jf9800458
  7. Fridovich I. 1975. Superoxide dismutase. Ann Rev Biochem 44: 147-159 https://doi.org/10.1146/annurev.bi.44.070175.001051
  8. Behar D, Czapski G, Raban J, Dorfman LM, Schwarz HA. 1970. The acid dissociation constant and decay kinetics of the perhydroxy radical. J Phys Chem 74: 3209-3213 https://doi.org/10.1021/j100711a009
  9. Schwarz M, Preres G, Kunz W, Furstenberger G, Kittstein W, Marks F. 1984. On the role of superoxide anion radicals in skin tumor promotion. Carcinogenesis 5: 1663-1670 https://doi.org/10.1093/carcin/5.12.1663
  10. Cerutti PA. 1985. Pro-oxidant states and tumor promotion. Science 227: 375-381 https://doi.org/10.1126/science.2981433
  11. Ohkuma M, Kajita S, Iizuka H. 1987. Superoxide dismutase in epidermis: its relation to keratinocyte proliferation. J Dermatol 14: 562-568 https://doi.org/10.1111/j.1346-8138.1987.tb03627.x
  12. Menvielle-Bourg FJ. 2005. Superoxide dismutase (SOD), a powerful antioxidant is now available orally. Phytotherapie 3: 1-4 https://doi.org/10.1007/s10298-005-0057-2
  13. Gustafson H, Johansson B, Edsmyr F. 1981. Peyronie's disease: experience of local treatment with orgotein. Eur Urol 7: 346-348 https://doi.org/10.1159/000473262
  14. Regnault C, Soursac M, Rock-Arveiller M, Postaire E, Hazebroucq G. 1996. Pharmacokinetics of superoxide dismutase in rats after oral administration. Biopharm Drug Dispos 17: 165-174 https://doi.org/10.1002/(SICI)1099-081X(199603)17:2<165::AID-BDD945>3.0.CO;2-N
  15. Vouldoukis I, Lacan D, Kamate C, Costec P, Calendaa A, Mazierb D, Contib M, Dugas B. 2004. Antioxidant and anti-inflammatory properties of a Cucumis melo LC. extract rich in superoxide dismutase activity. J Ethnopharmacol 94: 67-75 https://doi.org/10.1016/j.jep.2004.04.023
  16. Xu Y, Shao Y, Voorhees JJ, Fisher GJ. 2006. Oxidative inhibition of receptor-type protein-tyrosine phosphatase by ultraviolet irradiation activates epidermal growth factor receptor in human keratinocytes. J Biol Chem 281: 27389-27397 https://doi.org/10.1074/jbc.M602355200
  17. Ichihashi M, Ueda M, Budiyanto A, Bito T, Oka M, Fukunaga M, Tsuru K, Horikawa T. 2003. UV-induced skin damage. Toxicology 189: 21-39 https://doi.org/10.1016/S0300-483X(03)00150-1
  18. Youn JI. 1995. Effects of ultraviolet radiation on the skin. J Korean Asso Radiat Prot 20: 181-186
  19. Haber LC, Bickers DR. 1989. Principles of diagnosis and treatment. In Photosensitivity Diseases. BC Decker Inc, Toronto. p 308-314
  20. Berneburg M, Plettenberg H, Krutmann J. 2000. Photoaging of human skin. Photodermatol Photoimmunol Photomed 16: 239-244 https://doi.org/10.1034/j.1600-0781.2000.160601.x
  21. Sies H. 1986. Biochemistry of oxidative stress. Angewandte Chem 25: 1058-1071 https://doi.org/10.1002/anie.198610581
  22. Cho YH. 2005. Skin, nutrition and functional foods. Food Sci Ind 38: 8-15
  23. Kim NI. 2005. Role of vitamins and minerals on skin care and beauty. Food Sci Ind 38: 16-25
  24. Bogden JD, Bendich A, Kemp FW, Bruening KS, Skurnic JH, Denny T, Baker H, Louria DB. 1994. Daily micronutrient supplements enhance delayed hypersensitivity skin test responses in older people. Am J Clin Nutr 60: 437-447
  25. Karla W, Mohsen M. 1994. Evaluation of the photoprotective effect of oral vitamin E supplementation. Arch Dermatol 130: 1257-1261 https://doi.org/10.1001/archderm.130.10.1257
  26. Dugas B. 2002. Glisodin${(R)}$ a nutraceutical product that promotes the oral delivery of superoxide dismutase. Free Radic Biol Med 33: S64
  27. Muth CM, Glenz Y, Klaus M, Radermacher P, Speit G, Leverve X. 2004. Influence of an orally effective SOD on hyperbaric oxygen-related cell damage. Free Radic Res 38: 927-132 https://doi.org/10.1080/10715760412331273197
  28. Vouldoukis I, Conti M, Kolb JP, Calenda A, Mazier D, Dugaset B. 2003. Induction of Th1-dependent immunity by an orally effective melon superoxide dismutase extract. Curr Trends Immunol 5: 141-145
  29. Vouldoukis I, Conti M, Krauss P, Kamate C, Blazquez S, Tefit M, Mazier D, Calenda A, Dugas B. 2004. Supplementation with gliadin-combined plant superoxide dismutase extract promotes antioxidant defences and protects against oxidative stress. Phytother Res 18: 957-962 https://doi.org/10.1002/ptr.1542
  30. Youn JI, Choe YB, Park SB, Suh DH, Park YK, Ahn SK, Kim KH, Kim ST, Kim HY, Lee SC, Oh SJ, Kim JJ, Kang SH. 2000. The Fitzpatrick skin type in Korean people. Korean J Dermatol 38: 920-927
  31. Mac-Mary S, Sainthillier JM, Courderotmasuyer C, Creidi P, Humbert P. 2007. Could a photobiological test be a suitable method to assess the antioxidant effect of a nutritional supplement (Glisodin${(R)}$)? Eur J Derm 17: 254-255

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