Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Enhancement of Anticancer Effect through Photodynamic Therapy with High Oxygen Concentration

  • Kim, Yun-Ho (Department of Otolaryngology-Head & Neck Surgery, Dankook University) ;
  • Chung, Phil-Sang (Department of Otolaryngology-Head & Neck Surgery, Dankook University) ;
  • Lee, Sang-Joon (Department of Otolaryngology-Head & Neck Surgery, Dankook University) ;
  • Shin, Jang-In (Medical Laser and Device Research Center, Dankook University) ;
  • Hwang, Hee-Jun (Medical Laser and Device Research Center, Dankook University) ;
  • Ahn, Jin-Chul (Medical Laser and Device Research Center, Dankook University)
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  • 안진철 (단국대학교 의과대학 의학레이저 연구센터)
  • Published : 2009.03.31
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Abstract

In photodynamic therapy (PDT), oxygen plays important role. Because of singlet oxygen which is produced by activated photosensitizer after laser irradiation of specific wavelength. The aim of this study is to find how oxygen concentration affects anticancer effect in PDT. Groups were divided into PDT with oxygen applied group and only PDT applied group. PDT with oxygen applied group supplied oxygen for 15 minute before laser irradiation. In vitro, CT-26 cell was incubated with various concentration of photofrin $(50.0{\sim}0.05{\mu}g/ml)$ and was irradiated with 632nm diode laser 6hr after application of photofrin. The cell viability of two groups was assessed by MTT assay. In vivo, CT-26 cell line was transplanted into the subcutaneous tissue of BALB/c mouse. The anticancer effect of two groups was measured by tumor volume change. In vitro study, the cell viability was significantly decreased at $1.56{\sim}3.13{\mu}g/ml$ in PDT with oxygen applied group. In vivo study, the PDT with oxygen applied group significantly higher reduction rate of tumor volume 7 days after PDT compared to PDT only group. The high oxygen concentration might enhance the anticancer effect of the photodynamic therapy.

Keywords

References

  1. Ball DJ, Luo Y, Kessel D, Griffiths J, Brown SB, Vernon DI. The induction of apoptosis by a positively charged methylene blue derivative. J Photochem Photobiol B 1998. 42: 159-163. https://doi.org/10.1016/S1011-1344(98)00061-X
  2. Chung PS, Park ET, Lee KI, Ahn JC. ROS Formation and the mechanism of phtotodynamic therapy using 9-hydroxypheophorbide- a on AMC-HN3 human head and Neck carcinoma cell line. Korea J Otolaryngol. 2006. 49: 1194-2000.
  3. Dougherty TJ. An update on photodynamic therapy applications. J Clin Laser Med Surg. 2002. 20: 3-7. https://doi.org/10.1089/104454702753474931
  4. Foote CS. Type I and Type II mechanisms of photodynamic action. In: Heitz JR, Downum KR, editors. Light-Activates Pesticides. Washington D: Am Chem Soci. 1987. 22-38.
  5. Fuchs J, Thiele J. The role of oxygen in cutaneous photodynamic therapy. Free Radic Biol Med. 1998. 24: 835-847. https://doi.org/10.1016/S0891-5849(97)00370-5
  6. Hampton JA, Selman SH. Mechanism of cell killing in photodynamic therapy using a novel in vivo drug/in vitro light culture system. Photochem. Photobial. 1992. 56: 235-243. https://doi.org/10.1111/j.1751-1097.1992.tb02152.x
  7. Kelly JF, Snell ME. Hematoporphyrin derivative: a possible aid in the diagnosis and therapy of carcinoma of the bladder. J Urol.1976. 115: 150-151. https://doi.org/10.1016/S0022-5347(17)59108-9
  8. Kessel D, Luo Y, Deng Y, Chang CK. The role of subcellular localization in initiation of apoptosis by photodynamic therapy. Photochem Photobiol. 1997. 65: 422-426. https://doi.org/10.1111/j.1751-1097.1997.tb08581.x
  9. Lavie G, Kaplinsky C, Toren A, Aizman I, Meruelo, D, Mazur Y, Mandel M. A photodynamic pathway to apoptosis and necrosis induced by dimethyl tetrahydroxyhelianthrone and hypericin in leukaemic cells: possible relevance to photodynamic therapy. Br J Cancer 1999. 79: 423-432. https://doi.org/10.1038/sj.bjc.6690066
  10. Malham GM, Thomsen RJ, Finlay GJ, Baguley BC. Subcellular distribution and photocytotoxicity of aluminium phthalocyanines and haematoporphyrin derivative in cultured human meningioma cells. Br J Neurosurg. 1996. 10: 51-57. https://doi.org/10.1080/02688699650040520
  11. Moan J, Berg K. The photodegradation of porphyrins in cells can be used to estimate the lifetime of singlet oxygen. Photochem Photobiol. 1991. 53: 549-553. https://doi.org/10.1111/j.1751-1097.1991.tb03669.x
  12. Ochsner M. New trends in photobiology. Photophysical and photobiological processes in the photodynamic therapy of tumors. Photochem Photobiol. 1997. 39: 1-18. https://doi.org/10.1111/j.1751-1097.1984.tb03395.x
  13. Peng Q, Warloe T, Moan J, Godal A, Apricena F, Giercksky KE, Nesland JM. Antitumor effect of 5-aminolevulinic acidmediated photodynamic therapy can be enhanced by the use of a low dose of photofrin in human tumor xenografts. Cancer Res. 2001. 61: 5824-5832.
  14. Ripson RL, Blades EJ, Olsen AM. The use of hematoporphyrin in tumor destruction. J Natl Cancer Inst. 1961. 26: 1-11.
  15. Tromberg BJ, Orenstein A, Kimel S, Barker SJ, Hyatt J, Nelson JS, Berns MW. In vivo tumor oxygen tension measurements for the evaluation of the efficiency of photodynamic therapy. Photochem Photobiol. 1990. 52: 375-385. https://doi.org/10.1111/j.1751-1097.1990.tb04193.x
  16. Van Hillegersberg R, Kort WJ, Wilson JH. Current status of photodynamic therapy in oncology. Drugs 1994. 48: 510-527. https://doi.org/10.2165/00003495-199448040-00003
  17. Vaupel P, Schlenger K, Knoop C, Hockel M. Oxygenation of human tumors: evaluation of tissue oxygen distribution in breast cancers by computerized O2 tension measurements. Cancer Res. 1991. 51: 3316-3322.
  18. Weishaupt KR, Gomer CJ, Dougherty TJ. Identification of singlet oxygen as the cytotoxic agent in photoinactivation of a murine tumor. Cancer Res. 1976. 36: 2326-2329.
  19. Wyld L, Reed MW, Brown NJ. Differential cell death response to photodynamic therapy is dependent on dose and cell type. Br J Cancer 2001. 84: 1384-1386. https://doi.org/10.1054/bjoc.2001.1795