Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Human IL-27p28 Gene Polymorphisms are Associated with the Serum Total IgE Levels of Allergic Rhinitis Patients

사람의 IL-27p28 유전자 다형성은 알레르기성 비염 환자 혈청의 IgE 양과 연관됨

  • Yu, Ji-In (Department of Pathology, Wonkwang University School of Medicine) ;
  • Han, Weon-Cheol (Department of Pathology, Wonkwang University School of Medicine) ;
  • Lee, Jae-Hoon (Department of Otolaryngology, Wonkwang University School of Medicine) ;
  • Kim, Hun-Soo (Department of Pathology, Wonkwang University School of Medicine) ;
  • Yun, Ki-Jung (Digestive Disease Research Institute, Wonkwang University School of Medicine) ;
  • Lee, Jae-Hoon (Department of Internal Medicine, Wonkwang University School of Medicine) ;
  • Moon, Hyung-Bae (Department of Pathology, Wonkwang University School of Medicine) ;
  • Chae, Soo-Cheon (Department of Pathology, Wonkwang University School of Medicine)
  • 유지인 (원광대학교 의과대학 병리학교실) ;
  • 한원철 (원광대학교 의과대학 병리학교실) ;
  • 이재훈 (원광대학교 의과대학 이비인후과학교실) ;
  • 김헌수 (원광대학교 의과대학 병리학교실) ;
  • 윤기중 (원광대학교 의과대학 소화기질환연구소) ;
  • 이재훈 (원광대학교 의과대학 내과학교실) ;
  • 문형배 (원광대학교 의과대학 병리학교실) ;
  • 채수천 (원광대학교 의과대학 병리학교실)
  • Published : 2009.03.31
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Abstract

Interleukin 27 (IL-27) was discovered as a heterodimeric cytokine of the IL-12 family, and is composed of two subunits - Epstein-Barr virus induced gene 3 (EBI3) and p28. It acts as a versatile cytokine in the early regulation of Th1 initiation and in the negative regulation of the Th2 factor GATA binding protein 3 (GATA-3). This cytokine is mediated by the IL-27 receptor (WSX-1), which is highly expressed on $CD4^+$ T lymphocytes and NK cells. We previously identified four polymorphisms in the human IL-27p28 gene and suggested that the polymorphism of IL-27p28 is associated with susceptibility to asthma. To determine whether these IL-27p28 SNPs are associated with susceptibility to allergic rhinitis, the genotype and allele frequencies of IL-27p28 SNPs were analyzed between allergic rhinitis patients and healthy controls. Although the genotype and allele frequencies of IL-27p28 SNPs in allergic rhinitis patients were not significantly different from those of the control group, there was a suggestive difference (P=0.037) between these groups in total serum IgE levels in the g.2905T>G SNP of the IL-27p28 gene. Our result implies that the g.2905T>G SNP of the IL-27p28 gene might have an affect on IgE production in allergic rhinitis patients.

인터루킨 27(IL-27)은 인터루킨 12계열의 이성이량체 사이토카인으로 EBI3와 p28의 두 서브유니트로 구성 되어 있다. 인터루킨 27은 Th1 개시의 초기 조절에 관여하는 사이토카인으로 Th2 인자인 GATA-3의 작용을 억제하는 역할을 한다. 이 사이토카인은 $CD4^+$ T 림프구와 자연살해세포에서 아주 높게 발현하는 수용체(WSX-1)에 의하여 매개된다. 우리들은 사람의 IL-27p28유전자에서 네 개의 유전자다형성 부위를 찾아서 이들 유전자다형성이 기관지 천식의 감수성에 영향을 미치는 것을 보고한 바 있다. 이 연구에서는 IL-27p28 유전자의 유전자다형성이 알레르기성 비염의 감수성에 영향을 미치는 지를 알아보기 위하여 이들 다형성에 있어서 알레르기성 비염환자 군과 정상인군 사이의 유전자형 및 대립형질의 빈도를 비교분석 하였다. 비록 알레르기성 비염환자 군에서 IL-27p28 유전자의 유전자다형성의 유전자형 및 대립형질의 빈도의 차는 정상인 군의 그것과 큰 차이가 없었으나, g.2905T>G SNP에서 두 그룹간에 주목할만한 차이(P=0.037)를 발견하였다. 이 결과는 IL-27p28 유전자의 g.2905T>G 유전자 형성이 알레르기성 비염환자에서 IgE의 생산에 영향을 준다는 것을 암시한다.

Keywords

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