Journal of Acupuncture Research

  • 제26권1호
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  • Pages.153-162
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  • 2009
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  • 2586-288X(pISSN)
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  • 2586-2898(eISSN)
대한침구의학회 (Korean Acupuncture & Moxibustion Medicine Society)
권호 표지

VEGF 조절을 통한 육계 추출물의 신생혈관 형성 촉진 작용

Stimulatory Efect of Cinnamomum cassia Extract on Angiogenesis Through Regulation of VEGF

  • 고정민 (경희대학교 한의과대학 침구학교실) ;
  • 이상훈 (경희대학교 한의과대학 침구학교실) ;
  • 백용현 (경희대학교 한의과대학 침구학교실) ;
  • 최도영 (경희대학교 한의과대학 침구학교실)
  • Ko, Jeong-Min (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Sang-Hoon (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Baek, Yong-Hyeon (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Choi, Do-Young (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyung Hee University)
  • 발행 : 2009.02.20
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초록

목적 : 한의학 분야에서 육계(CCE: Cinnamomum cassia)는 혈류 순환이 저하된 상태에 널리 쓰이는 약재이다. 이 연구에서는 CCE와 그 활성 혼합물인 cinnamic acid가 in vitro와 in vivo에서 발휘하는 신생혈관 형성 작용에 대해 알아보고자 한다. 방법 : CCE와 CA의 신생혈관형성 작용을 알아보기 위하여 Human unbilical endothelial cells(HUVECs)와 Bovine arterial endothelial cells(BAECs) 그리고 Matrigel assay를 이용하였다. 결과 : In vitro에서 CCE와 그 활성 혼합물은 HUVEC 증식, 이동, 모세혈관-유사 관 형성을 유도한다. CCE에서의 25-50% 증가(1 또는 10ng/ml 용량에서)는 CA에서 증식과 유사하게 나타났다. HUVEC에서는 증식이 뚜렷하게 나타났으나 BAEC에서는 증식이 뚜렷하게 나타나지 않아 증식 효과는 내피세포의 기원에 따라 다른 것으로 나타났다. BAEC는 CCE와 CA에서 증식은 보이지 않았지만 이동과 모세혈관-유사 관 형성 또는 분화를 보였다. 또한 CCE와 CA는 HUVEC에서 VEGF 발현과 Flk-1/KDR 발현을 각각 2.2배와 2.5배 증가시켰다. 결론 : CCE와 그 활성 혼합물은 in vivo와 in vitro에서 혈관 내피세포의 VEGF 발현을 통하여 신생혈관 형성 작용을 유도하며 이는 앞으로 다른 한약 추출물의 신생혈관형성 촉진 작용 연구에 모델을 제시할 것으로 보인다.

키워드

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